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Requirements for CD1d recognition by human invariant Valpha24+ CD4-CD8- T cells.

Exley M, Garcia J, Balk SP, Porcelli S - J. Exp. Med. (1997)

Bottom Line: Upon activation by anti-CD3 or CD1d, the clones produced both Th1 and Th2 cytokines.These results demonstrate that human invariant Valpha24+ CD4-CD8- T cells, and presumably the homologous murine NK1+ T cell population, are CD1d reactive and functionally distinct from NK cells.The conservation of this cell population and of the CD1d ligand across species indicates an important immunological function.

View Article: PubMed Central - PubMed

Affiliation: Cancer Biology Program, Hematology/Oncology Division, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.

ABSTRACT
A subset of human CD4-CD8- T cells that expresses an invariant Valpha24-JalphaQ T cell receptor (TCR)-alpha chain, paired predominantly with Vbeta11, has been identified. A series of these Valpha24 Vbeta11 clones were shown to have TCR-beta CDR3 diversity and express the natural killer (NK) locus-encoded C-type lectins NKR-P1A, CD94, and CD69. However, in contrast to NK cells, they did not express killer inhibitory receptors, CD16, CD56, or CD57. All invariant Valpha24(+) clones recognized the MHC class I-like CD16 molecule and discriminated between CD1d and other closely related human CD1 proteins, indicating that recognition was TCR-mediated. Recognition was not dependent upon an endosomal targeting motif in the cytoplasmic tail of CD1d. Upon activation by anti-CD3 or CD1d, the clones produced both Th1 and Th2 cytokines. These results demonstrate that human invariant Valpha24+ CD4-CD8- T cells, and presumably the homologous murine NK1+ T cell population, are CD1d reactive and functionally distinct from NK cells. The conservation of this cell population and of the CD1d ligand across species indicates an important immunological function.

Show MeSH
Invariant Vα24+ TCR-α nucleotide and derived amino acid  sequences. DN invariant Vα24+ T cell clone cDNA was sequenced. One  clone (DN2.C7) had a distinct nucleotide sequence, which resulted in an  identical amino acid sequence as shown.
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Figure 1: Invariant Vα24+ TCR-α nucleotide and derived amino acid sequences. DN invariant Vα24+ T cell clone cDNA was sequenced. One clone (DN2.C7) had a distinct nucleotide sequence, which resulted in an identical amino acid sequence as shown.

Mentions: The TCR structure of a series of eight DN Vα24+ Vβ11+ clones from donor 2 and single clones from donors 1 and 3 was analyzed. Sequence analysis demonstrated that the DN clones from donors 1 and 3 and all but one of the clones from donor 2 expressed the invariant Vα24 TCR-α chain (Table 1). Significantly, in one invariant Vα24+ clone (DN2.C7), the Vα24-encoded serine was apparently removed during recombination and the serine was regenerated through an N-region addition and recombination 2 bp further 5′ in JαQ (Fig. 1). These results confirmed the high frequency of the invariant Vα24 TCR among DN Vα24+ T cells, and suggested that this TCR is strongly selected based upon its protein structure, rather than being generated exclusively by a developmentally programmed precise joining of Vα24 and JαQ gene segments.


Requirements for CD1d recognition by human invariant Valpha24+ CD4-CD8- T cells.

Exley M, Garcia J, Balk SP, Porcelli S - J. Exp. Med. (1997)

Invariant Vα24+ TCR-α nucleotide and derived amino acid  sequences. DN invariant Vα24+ T cell clone cDNA was sequenced. One  clone (DN2.C7) had a distinct nucleotide sequence, which resulted in an  identical amino acid sequence as shown.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2198960&req=5

Figure 1: Invariant Vα24+ TCR-α nucleotide and derived amino acid sequences. DN invariant Vα24+ T cell clone cDNA was sequenced. One clone (DN2.C7) had a distinct nucleotide sequence, which resulted in an identical amino acid sequence as shown.
Mentions: The TCR structure of a series of eight DN Vα24+ Vβ11+ clones from donor 2 and single clones from donors 1 and 3 was analyzed. Sequence analysis demonstrated that the DN clones from donors 1 and 3 and all but one of the clones from donor 2 expressed the invariant Vα24 TCR-α chain (Table 1). Significantly, in one invariant Vα24+ clone (DN2.C7), the Vα24-encoded serine was apparently removed during recombination and the serine was regenerated through an N-region addition and recombination 2 bp further 5′ in JαQ (Fig. 1). These results confirmed the high frequency of the invariant Vα24 TCR among DN Vα24+ T cells, and suggested that this TCR is strongly selected based upon its protein structure, rather than being generated exclusively by a developmentally programmed precise joining of Vα24 and JαQ gene segments.

Bottom Line: Upon activation by anti-CD3 or CD1d, the clones produced both Th1 and Th2 cytokines.These results demonstrate that human invariant Valpha24+ CD4-CD8- T cells, and presumably the homologous murine NK1+ T cell population, are CD1d reactive and functionally distinct from NK cells.The conservation of this cell population and of the CD1d ligand across species indicates an important immunological function.

View Article: PubMed Central - PubMed

Affiliation: Cancer Biology Program, Hematology/Oncology Division, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.

ABSTRACT
A subset of human CD4-CD8- T cells that expresses an invariant Valpha24-JalphaQ T cell receptor (TCR)-alpha chain, paired predominantly with Vbeta11, has been identified. A series of these Valpha24 Vbeta11 clones were shown to have TCR-beta CDR3 diversity and express the natural killer (NK) locus-encoded C-type lectins NKR-P1A, CD94, and CD69. However, in contrast to NK cells, they did not express killer inhibitory receptors, CD16, CD56, or CD57. All invariant Valpha24(+) clones recognized the MHC class I-like CD16 molecule and discriminated between CD1d and other closely related human CD1 proteins, indicating that recognition was TCR-mediated. Recognition was not dependent upon an endosomal targeting motif in the cytoplasmic tail of CD1d. Upon activation by anti-CD3 or CD1d, the clones produced both Th1 and Th2 cytokines. These results demonstrate that human invariant Valpha24+ CD4-CD8- T cells, and presumably the homologous murine NK1+ T cell population, are CD1d reactive and functionally distinct from NK cells. The conservation of this cell population and of the CD1d ligand across species indicates an important immunological function.

Show MeSH