Let's make Golgi.
Bottom Line: Does the Golgi self-organize or does it form around an instructive template?Evidence on both sides is piling up, but a definitive conclusion is proving elusive.
Does the Golgi self-organize or does it form around an instructive template? Evidence on both sides is piling up, but a definitive conclusion is proving elusive.
Mentions: Two papers in this issue contest the matrix interpretation. Brian Storrie (Virginia Tech, Blacksburg, VA) uses a higher concentration of the Sar1 mutant used by Warren (which is stuck in the active, GTP-loaded state) and finds that now both Golgi enzymes and matrix proteins leave the Golgi (see Miles et al. on page 543 and Fig. 1). Lippincott-Schwartz uses an alternative Sar1 mutant, this one stuck in the inactive, GDP-loaded state, to show that Golgi enzymes and matrix proteins can be forced back into the ER (see Ward et al. on page 557 and Fig. 2). In FRAP experiments, Ward et al. also show that Golgi matrix proteins can exchange dynamically rather than acting as a static matrix.