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Ordering of human bone marrow B lymphocyte precursors by single-cell polymerase chain reaction analyses of the rearrangement status of the immunoglobulin H and L chain gene loci.

Ghia P, ten Boekel E, Sanz E, de la Hera A, Rolink A, Melchers F - J. Exp. Med. (1996)

Bottom Line: CD19+CD10+ human B lineage bone marrow cells were separated into cycling or resting cells, which differ in their expression of CD34, VpreB, recombination activating gene (RAG-1), and terminal deoxynucleotidyl transferase (TdT).Polymerase chain reaction analyses developed for DHJH and VkJk, VkJkK(de) and VkK(de) rearrangements with DNA of single cells and a comparison with B lineage cell development in mouse bone marrow, allow to delineate the human B lymphocyte pathway of development as follows: CD34+VpreB+RAG-1+TdT+, DHJH-rearranged, kL germline cycling pre-B I cells-->CD34-VpreB+microH chain+ (pre-B receptor+) RAG-1-TdT-, VHDHJH-rearranged, kL germline, cycling pre-B II cells-->CD34-VpreB-, intracytoplasmic microH chain+ (pre-B receptor-) RAG-1+/-TdT-, VHDHJH-rearranged, mainly kL germline cycling pre-B II cells-->CD34-VpreB-intracytoplasmic microH chain+, RAG-1+TdT-, VHDHJH-rearranged, VkJk-rearranged, IgM-, resting pre-B II cells CD34+VpreB-, sIgM+, RAG-1+TdT-, VHDHJH- and VkJk-rearranged IgM+ immature B cells-->CD34-, CD10-, sIgM+/sIgD+ mature B cells.This order, for the first time established for human B lineage cells, shows striking similarities with that established for mouse B lineage cells in bone marrow.

View Article: PubMed Central - PubMed

Affiliation: Basel Institute for Immunology, Switzerland.

ABSTRACT
CD19+CD10+ human B lineage bone marrow cells were separated into cycling or resting cells, which differ in their expression of CD34, VpreB, recombination activating gene (RAG-1), and terminal deoxynucleotidyl transferase (TdT). Polymerase chain reaction analyses developed for DHJH and VkJk, VkJkK(de) and VkK(de) rearrangements with DNA of single cells and a comparison with B lineage cell development in mouse bone marrow, allow to delineate the human B lymphocyte pathway of development as follows: CD34+VpreB+RAG-1+TdT+, DHJH-rearranged, kL germline cycling pre-B I cells-->CD34-VpreB+microH chain+ (pre-B receptor+) RAG-1-TdT-, VHDHJH-rearranged, kL germline, cycling pre-B II cells-->CD34-VpreB-, intracytoplasmic microH chain+ (pre-B receptor-) RAG-1+/-TdT-, VHDHJH-rearranged, mainly kL germline cycling pre-B II cells-->CD34-VpreB-intracytoplasmic microH chain+, RAG-1+TdT-, VHDHJH-rearranged, VkJk-rearranged, IgM-, resting pre-B II cells CD34+VpreB-, sIgM+, RAG-1+TdT-, VHDHJH- and VkJk-rearranged IgM+ immature B cells-->CD34-, CD10-, sIgM+/sIgD+ mature B cells. This order, for the first time established for human B lineage cells, shows striking similarities with that established for mouse B lineage cells in bone marrow.

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(a) Frequencies of CD19+ CD10+ B lineage cells and their subpopulations among total lymphoid cell compartment in human bone marrow  of donors of different ages (one donor = one symbol). The frequencies of these subpopulations among all CD19+ CD10+ cells are also given. (b) Frequencies of CD19+CD10−sIgM+ mature B cells in the lymphoid compartments of human bone marrow, and their relative contribution to all CD19+  cells in human bone marrow of donors of different ages.
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Figure 2: (a) Frequencies of CD19+ CD10+ B lineage cells and their subpopulations among total lymphoid cell compartment in human bone marrow of donors of different ages (one donor = one symbol). The frequencies of these subpopulations among all CD19+ CD10+ cells are also given. (b) Frequencies of CD19+CD10−sIgM+ mature B cells in the lymphoid compartments of human bone marrow, and their relative contribution to all CD19+ cells in human bone marrow of donors of different ages.

Mentions: The representative of the CD19+CD10+ B lineage subpopulations of human bone marrow described above was analyzed in 53 donors who were 10 mo to 76 yr old. The results are presented in Fig. 2 a as the percentage values of cells within the lymphoid compartments, and as the percentage values of cells among the CD19+CD10+ B lineage precursor compartments in bone marrow.


Ordering of human bone marrow B lymphocyte precursors by single-cell polymerase chain reaction analyses of the rearrangement status of the immunoglobulin H and L chain gene loci.

Ghia P, ten Boekel E, Sanz E, de la Hera A, Rolink A, Melchers F - J. Exp. Med. (1996)

(a) Frequencies of CD19+ CD10+ B lineage cells and their subpopulations among total lymphoid cell compartment in human bone marrow  of donors of different ages (one donor = one symbol). The frequencies of these subpopulations among all CD19+ CD10+ cells are also given. (b) Frequencies of CD19+CD10−sIgM+ mature B cells in the lymphoid compartments of human bone marrow, and their relative contribution to all CD19+  cells in human bone marrow of donors of different ages.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2196361&req=5

Figure 2: (a) Frequencies of CD19+ CD10+ B lineage cells and their subpopulations among total lymphoid cell compartment in human bone marrow of donors of different ages (one donor = one symbol). The frequencies of these subpopulations among all CD19+ CD10+ cells are also given. (b) Frequencies of CD19+CD10−sIgM+ mature B cells in the lymphoid compartments of human bone marrow, and their relative contribution to all CD19+ cells in human bone marrow of donors of different ages.
Mentions: The representative of the CD19+CD10+ B lineage subpopulations of human bone marrow described above was analyzed in 53 donors who were 10 mo to 76 yr old. The results are presented in Fig. 2 a as the percentage values of cells within the lymphoid compartments, and as the percentage values of cells among the CD19+CD10+ B lineage precursor compartments in bone marrow.

Bottom Line: CD19+CD10+ human B lineage bone marrow cells were separated into cycling or resting cells, which differ in their expression of CD34, VpreB, recombination activating gene (RAG-1), and terminal deoxynucleotidyl transferase (TdT).Polymerase chain reaction analyses developed for DHJH and VkJk, VkJkK(de) and VkK(de) rearrangements with DNA of single cells and a comparison with B lineage cell development in mouse bone marrow, allow to delineate the human B lymphocyte pathway of development as follows: CD34+VpreB+RAG-1+TdT+, DHJH-rearranged, kL germline cycling pre-B I cells-->CD34-VpreB+microH chain+ (pre-B receptor+) RAG-1-TdT-, VHDHJH-rearranged, kL germline, cycling pre-B II cells-->CD34-VpreB-, intracytoplasmic microH chain+ (pre-B receptor-) RAG-1+/-TdT-, VHDHJH-rearranged, mainly kL germline cycling pre-B II cells-->CD34-VpreB-intracytoplasmic microH chain+, RAG-1+TdT-, VHDHJH-rearranged, VkJk-rearranged, IgM-, resting pre-B II cells CD34+VpreB-, sIgM+, RAG-1+TdT-, VHDHJH- and VkJk-rearranged IgM+ immature B cells-->CD34-, CD10-, sIgM+/sIgD+ mature B cells.This order, for the first time established for human B lineage cells, shows striking similarities with that established for mouse B lineage cells in bone marrow.

View Article: PubMed Central - PubMed

Affiliation: Basel Institute for Immunology, Switzerland.

ABSTRACT
CD19+CD10+ human B lineage bone marrow cells were separated into cycling or resting cells, which differ in their expression of CD34, VpreB, recombination activating gene (RAG-1), and terminal deoxynucleotidyl transferase (TdT). Polymerase chain reaction analyses developed for DHJH and VkJk, VkJkK(de) and VkK(de) rearrangements with DNA of single cells and a comparison with B lineage cell development in mouse bone marrow, allow to delineate the human B lymphocyte pathway of development as follows: CD34+VpreB+RAG-1+TdT+, DHJH-rearranged, kL germline cycling pre-B I cells-->CD34-VpreB+microH chain+ (pre-B receptor+) RAG-1-TdT-, VHDHJH-rearranged, kL germline, cycling pre-B II cells-->CD34-VpreB-, intracytoplasmic microH chain+ (pre-B receptor-) RAG-1+/-TdT-, VHDHJH-rearranged, mainly kL germline cycling pre-B II cells-->CD34-VpreB-intracytoplasmic microH chain+, RAG-1+TdT-, VHDHJH-rearranged, VkJk-rearranged, IgM-, resting pre-B II cells CD34+VpreB-, sIgM+, RAG-1+TdT-, VHDHJH- and VkJk-rearranged IgM+ immature B cells-->CD34-, CD10-, sIgM+/sIgD+ mature B cells. This order, for the first time established for human B lineage cells, shows striking similarities with that established for mouse B lineage cells in bone marrow.

Show MeSH
Related in: MedlinePlus