Limits...
Identification of a committed T cell precursor population in adult human peripheral blood.

Bruno L, Res P, Dessing M, Cella M, Spits H - J. Exp. Med. (1997)

Bottom Line: At a molecular level, they express the pre-T cell receptor alpha (pT alpha) gene, CD3-gamma, CD-delta and CD-epsilon, and RAG-1 recombination enzyme and have initiated rearrangements in the T cell receptor (TCR)-beta locus (D-J).Moreover, low levels of CD3 epsilon protein, but not of TCR-beta chain, can be detected in their cytoplasm.Our results suggest that CD4+CD3- cells identified in peripheral blood are different from CD3-CD4+CD8- thymocytes and may contain precursors of an extrathymic T cell differentiation pathway.

View Article: PubMed Central - PubMed

Affiliation: Basel Institute for Immunology, Switzerland.

ABSTRACT
Here, we report data concerning the discovery in adult human peripheral blood of a precursor cell population able to differentiate into CD4+CD3+ alpha beta + mature T cells. These cells, which represent 0.1-0.5% of total peripheral blood mononuclear cells (PBMC), express substantial levels of CD4, but lack CD3 surface expression. At a molecular level, they express the pre-T cell receptor alpha (pT alpha) gene, CD3-gamma, CD-delta and CD-epsilon, and RAG-1 recombination enzyme and have initiated rearrangements in the T cell receptor (TCR)-beta locus (D-J). Moreover, low levels of CD3 epsilon protein, but not of TCR-beta chain, can be detected in their cytoplasm. Our results suggest that CD4+CD3- cells identified in peripheral blood are different from CD3-CD4+CD8- thymocytes and may contain precursors of an extrathymic T cell differentiation pathway.

Show MeSH
CD4+CD3−CD14− cell population expresses RAG-1 and is  not cycling. (A) RT–PCR analysis on two different samples of sorted  CD4+CD3−CD14− PBMC (1, 2), mature T cells (CD4+CD3+) and DN  thymocytes (DN Thymus). PCR products were blotted and probed with a  RAG-1-specific oligonucleotide. (B) Cell cycle analysis on sorted  CD4+CD3−CD14− (top) and on Jurkat T cell line (bottom).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2196171&req=5

Figure 3: CD4+CD3−CD14− cell population expresses RAG-1 and is not cycling. (A) RT–PCR analysis on two different samples of sorted CD4+CD3−CD14− PBMC (1, 2), mature T cells (CD4+CD3+) and DN thymocytes (DN Thymus). PCR products were blotted and probed with a RAG-1-specific oligonucleotide. (B) Cell cycle analysis on sorted CD4+CD3−CD14− (top) and on Jurkat T cell line (bottom).

Mentions: Previous studies have shown that analysis of the expression of RAG-1 and RAG-2 genes and of TCR transcription are useful to trace early events during T cell development both in murine and in human T cell development (7, 16). As independent confirmation of DJβ rearrangement of the TCR-β locus of CD4+CD3−CD14− cells, we tested for RAG-1 gene expression in two different preparations of CD4+CD3−CD14− cells from human peripheral blood. RAG-1 message could indeed be detected in both preparations (Fig. 3 A, lanes 1 and 2), as well as in DN cells from thymus, but not in mature CD4+CD3+ T cells.


Identification of a committed T cell precursor population in adult human peripheral blood.

Bruno L, Res P, Dessing M, Cella M, Spits H - J. Exp. Med. (1997)

CD4+CD3−CD14− cell population expresses RAG-1 and is  not cycling. (A) RT–PCR analysis on two different samples of sorted  CD4+CD3−CD14− PBMC (1, 2), mature T cells (CD4+CD3+) and DN  thymocytes (DN Thymus). PCR products were blotted and probed with a  RAG-1-specific oligonucleotide. (B) Cell cycle analysis on sorted  CD4+CD3−CD14− (top) and on Jurkat T cell line (bottom).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2196171&req=5

Figure 3: CD4+CD3−CD14− cell population expresses RAG-1 and is not cycling. (A) RT–PCR analysis on two different samples of sorted CD4+CD3−CD14− PBMC (1, 2), mature T cells (CD4+CD3+) and DN thymocytes (DN Thymus). PCR products were blotted and probed with a RAG-1-specific oligonucleotide. (B) Cell cycle analysis on sorted CD4+CD3−CD14− (top) and on Jurkat T cell line (bottom).
Mentions: Previous studies have shown that analysis of the expression of RAG-1 and RAG-2 genes and of TCR transcription are useful to trace early events during T cell development both in murine and in human T cell development (7, 16). As independent confirmation of DJβ rearrangement of the TCR-β locus of CD4+CD3−CD14− cells, we tested for RAG-1 gene expression in two different preparations of CD4+CD3−CD14− cells from human peripheral blood. RAG-1 message could indeed be detected in both preparations (Fig. 3 A, lanes 1 and 2), as well as in DN cells from thymus, but not in mature CD4+CD3+ T cells.

Bottom Line: At a molecular level, they express the pre-T cell receptor alpha (pT alpha) gene, CD3-gamma, CD-delta and CD-epsilon, and RAG-1 recombination enzyme and have initiated rearrangements in the T cell receptor (TCR)-beta locus (D-J).Moreover, low levels of CD3 epsilon protein, but not of TCR-beta chain, can be detected in their cytoplasm.Our results suggest that CD4+CD3- cells identified in peripheral blood are different from CD3-CD4+CD8- thymocytes and may contain precursors of an extrathymic T cell differentiation pathway.

View Article: PubMed Central - PubMed

Affiliation: Basel Institute for Immunology, Switzerland.

ABSTRACT
Here, we report data concerning the discovery in adult human peripheral blood of a precursor cell population able to differentiate into CD4+CD3+ alpha beta + mature T cells. These cells, which represent 0.1-0.5% of total peripheral blood mononuclear cells (PBMC), express substantial levels of CD4, but lack CD3 surface expression. At a molecular level, they express the pre-T cell receptor alpha (pT alpha) gene, CD3-gamma, CD-delta and CD-epsilon, and RAG-1 recombination enzyme and have initiated rearrangements in the T cell receptor (TCR)-beta locus (D-J). Moreover, low levels of CD3 epsilon protein, but not of TCR-beta chain, can be detected in their cytoplasm. Our results suggest that CD4+CD3- cells identified in peripheral blood are different from CD3-CD4+CD8- thymocytes and may contain precursors of an extrathymic T cell differentiation pathway.

Show MeSH