Limits...
Dendritic cells enhance growth and differentiation of CD40-activated B lymphocytes.

Dubois B, Vanbervliet B, Fayette J, Massacrier C, Van Kooten C, Brière F, Banchereau J, Caux C - J. Exp. Med. (1997)

Bottom Line: Furthermore, after CD40 ligation, DC enhanced by 30-300-fold the secretion of IgG and IgA by sIgD- B cells (essentially memory B cells).In the presence of DC, naive sIgD+ B cells produced, in response to interleukin-2, large amounts of IgM.Thus, in addition to activating naive T cells in the extrafollicular areas of secondary lymphoid organs, DC may directly modulate B cell growth and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Schering Plough, Laboratory for Immunological Research, Dardilly, France.

ABSTRACT
After antigen capture, dendritic cells (DC) migrate into T cell-rich areas of secondary lymphoid organs, where they induce T cell activation, that subsequently drives B cell activation. Here, we investigate whether DC, generated in vitro, can directly modulate B cell responses, using CD40L-transfected L cells as surrogate activated T cells. DC, through the production of soluble mediators, stimulated by 3- to 6-fold the proliferation and subsequent recovery of B cells. Furthermore, after CD40 ligation, DC enhanced by 30-300-fold the secretion of IgG and IgA by sIgD- B cells (essentially memory B cells). In the presence of DC, naive sIgD+ B cells produced, in response to interleukin-2, large amounts of IgM. Thus, in addition to activating naive T cells in the extrafollicular areas of secondary lymphoid organs, DC may directly modulate B cell growth and differentiation.

Show MeSH

Related in: MedlinePlus

D–Lc enhance both proliferation and differentiation of B cells activated with a soluble form of  CD40L. A soluble form of CD40L (sCD40L) was expressed as a dimeric fusion protein between the  mouse CD8α and the human CD40L. sCD40L was  used as a supernatant of COS cells at a final concentration of 25% (right) to stimulate (A) B cell proliferation (104 cells per well) and (B) B cell differentiation  (104 cells per well). IL-2 and IL-10 were added at  concentration indicated in Materials and Methods.  Results obtained with CD40L L cells are indicated for  comparison (left). (results from 1 experiment representative of 3). No significant B cell proliferation and  differentiation was observed in absence of CD40 engagement.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2196162&req=5

Figure 6: D–Lc enhance both proliferation and differentiation of B cells activated with a soluble form of CD40L. A soluble form of CD40L (sCD40L) was expressed as a dimeric fusion protein between the mouse CD8α and the human CD40L. sCD40L was used as a supernatant of COS cells at a final concentration of 25% (right) to stimulate (A) B cell proliferation (104 cells per well) and (B) B cell differentiation (104 cells per well). IL-2 and IL-10 were added at concentration indicated in Materials and Methods. Results obtained with CD40L L cells are indicated for comparison (left). (results from 1 experiment representative of 3). No significant B cell proliferation and differentiation was observed in absence of CD40 engagement.

Mentions: To analyze a possible contribution of the fibroblastic cell line (CD40L L cells), CD40 activation was carried out with a soluble form of CD40L using a fusion protein between the mouse CD8-α and the human CD40L. The soluble protein was a less efficient activator than the CD40L L cells (Fig. 6), particularly for proliferation of B cells, probably reflecting differences in the mode of CD40 engagement. Yet, addition of D–Lc resulted in enhanced B cell proliferation (Fig. 6 A) and differentiation (Fig. 6 B). The effect was particularly remarkable when cytokines, such as IL-2 or IL-10, were added to the cultures. Thus, the presence of the fibroblastic line is not mandatory for the B cell to undergo growth and differentiation in response to surrogate activated T cells (in the form of soluble CD40L and recombinant cytokines) and D–Lc.


Dendritic cells enhance growth and differentiation of CD40-activated B lymphocytes.

Dubois B, Vanbervliet B, Fayette J, Massacrier C, Van Kooten C, Brière F, Banchereau J, Caux C - J. Exp. Med. (1997)

D–Lc enhance both proliferation and differentiation of B cells activated with a soluble form of  CD40L. A soluble form of CD40L (sCD40L) was expressed as a dimeric fusion protein between the  mouse CD8α and the human CD40L. sCD40L was  used as a supernatant of COS cells at a final concentration of 25% (right) to stimulate (A) B cell proliferation (104 cells per well) and (B) B cell differentiation  (104 cells per well). IL-2 and IL-10 were added at  concentration indicated in Materials and Methods.  Results obtained with CD40L L cells are indicated for  comparison (left). (results from 1 experiment representative of 3). No significant B cell proliferation and  differentiation was observed in absence of CD40 engagement.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2196162&req=5

Figure 6: D–Lc enhance both proliferation and differentiation of B cells activated with a soluble form of CD40L. A soluble form of CD40L (sCD40L) was expressed as a dimeric fusion protein between the mouse CD8α and the human CD40L. sCD40L was used as a supernatant of COS cells at a final concentration of 25% (right) to stimulate (A) B cell proliferation (104 cells per well) and (B) B cell differentiation (104 cells per well). IL-2 and IL-10 were added at concentration indicated in Materials and Methods. Results obtained with CD40L L cells are indicated for comparison (left). (results from 1 experiment representative of 3). No significant B cell proliferation and differentiation was observed in absence of CD40 engagement.
Mentions: To analyze a possible contribution of the fibroblastic cell line (CD40L L cells), CD40 activation was carried out with a soluble form of CD40L using a fusion protein between the mouse CD8-α and the human CD40L. The soluble protein was a less efficient activator than the CD40L L cells (Fig. 6), particularly for proliferation of B cells, probably reflecting differences in the mode of CD40 engagement. Yet, addition of D–Lc resulted in enhanced B cell proliferation (Fig. 6 A) and differentiation (Fig. 6 B). The effect was particularly remarkable when cytokines, such as IL-2 or IL-10, were added to the cultures. Thus, the presence of the fibroblastic line is not mandatory for the B cell to undergo growth and differentiation in response to surrogate activated T cells (in the form of soluble CD40L and recombinant cytokines) and D–Lc.

Bottom Line: Furthermore, after CD40 ligation, DC enhanced by 30-300-fold the secretion of IgG and IgA by sIgD- B cells (essentially memory B cells).In the presence of DC, naive sIgD+ B cells produced, in response to interleukin-2, large amounts of IgM.Thus, in addition to activating naive T cells in the extrafollicular areas of secondary lymphoid organs, DC may directly modulate B cell growth and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Schering Plough, Laboratory for Immunological Research, Dardilly, France.

ABSTRACT
After antigen capture, dendritic cells (DC) migrate into T cell-rich areas of secondary lymphoid organs, where they induce T cell activation, that subsequently drives B cell activation. Here, we investigate whether DC, generated in vitro, can directly modulate B cell responses, using CD40L-transfected L cells as surrogate activated T cells. DC, through the production of soluble mediators, stimulated by 3- to 6-fold the proliferation and subsequent recovery of B cells. Furthermore, after CD40 ligation, DC enhanced by 30-300-fold the secretion of IgG and IgA by sIgD- B cells (essentially memory B cells). In the presence of DC, naive sIgD+ B cells produced, in response to interleukin-2, large amounts of IgM. Thus, in addition to activating naive T cells in the extrafollicular areas of secondary lymphoid organs, DC may directly modulate B cell growth and differentiation.

Show MeSH
Related in: MedlinePlus