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Dendritic cells enhance growth and differentiation of CD40-activated B lymphocytes.

Dubois B, Vanbervliet B, Fayette J, Massacrier C, Van Kooten C, Brière F, Banchereau J, Caux C - J. Exp. Med. (1997)

Bottom Line: Furthermore, after CD40 ligation, DC enhanced by 30-300-fold the secretion of IgG and IgA by sIgD- B cells (essentially memory B cells).In the presence of DC, naive sIgD+ B cells produced, in response to interleukin-2, large amounts of IgM.Thus, in addition to activating naive T cells in the extrafollicular areas of secondary lymphoid organs, DC may directly modulate B cell growth and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Schering Plough, Laboratory for Immunological Research, Dardilly, France.

ABSTRACT
After antigen capture, dendritic cells (DC) migrate into T cell-rich areas of secondary lymphoid organs, where they induce T cell activation, that subsequently drives B cell activation. Here, we investigate whether DC, generated in vitro, can directly modulate B cell responses, using CD40L-transfected L cells as surrogate activated T cells. DC, through the production of soluble mediators, stimulated by 3- to 6-fold the proliferation and subsequent recovery of B cells. Furthermore, after CD40 ligation, DC enhanced by 30-300-fold the secretion of IgG and IgA by sIgD- B cells (essentially memory B cells). In the presence of DC, naive sIgD+ B cells produced, in response to interleukin-2, large amounts of IgM. Thus, in addition to activating naive T cells in the extrafollicular areas of secondary lymphoid organs, DC may directly modulate B cell growth and differentiation.

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D–Lc are more efficient than monocytes in enhancing CD40-induced B cell differentiation. D–Lc, one EBV cell line, and elutriated monocytes were compared, after irradiation, in their ability to enhance (A) cytokine-independent CD40-induced B cell proliferation, (B) cytokine-independent IgG production, and (C) IL-2–dependent IgM production. Increasing numbers of stimulator cells were added to 104 highly purified B cells cultured  over 2.5 × 103 irradiated CD40L L cells. Results are expressed as mean of triplicate cultures (SD ⩽10%). (results from 1 experiment representative of 3).  No significant B cell proliferation and differentiation was observed in absence of CD40L L cells.
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Figure 5: D–Lc are more efficient than monocytes in enhancing CD40-induced B cell differentiation. D–Lc, one EBV cell line, and elutriated monocytes were compared, after irradiation, in their ability to enhance (A) cytokine-independent CD40-induced B cell proliferation, (B) cytokine-independent IgG production, and (C) IL-2–dependent IgM production. Increasing numbers of stimulator cells were added to 104 highly purified B cells cultured over 2.5 × 103 irradiated CD40L L cells. Results are expressed as mean of triplicate cultures (SD ⩽10%). (results from 1 experiment representative of 3). No significant B cell proliferation and differentiation was observed in absence of CD40L L cells.

Mentions: We compared the effects of various antigen-presenting cells including D–Lc, elutriated monocytes (>90% CD14), and lymphoblastoid cell lines (EBV–LCL) on the proliferation and differentiation of CD40-activated B cells. As shown in Fig. 5 A, monocytes were as potent as D–Lc in enhancing CD40induced B cell proliferation, whereas EBV cell lines were totally inefficient (only one of the three tested is shown in Fig. 5). Significant IgG production, in absence of cytokine, was observed with 3.5 × 102–103 D–Lc, whereas more than 5 × 103 monocytes were required to observe the same effects. At higher cell density (104 cells), monocytes induced levels of IgG in the order of 5 μg/ml compared with 17 μg/ml for D–Lc (Fig. 5 B). Concerning the IL-2–dependent IgM production, a significant effect was observed with less than 450 D–Lc, whereas more than 2.5 × 103 monocytes were required to reach similar levels of IgM secretion. Thus, whereas D–Lc and monocytes shared an equal ability to enhance B cell proliferation, D–Lc are more efficient that monocytes in inducing B cells to secrete IgG and IgM.


Dendritic cells enhance growth and differentiation of CD40-activated B lymphocytes.

Dubois B, Vanbervliet B, Fayette J, Massacrier C, Van Kooten C, Brière F, Banchereau J, Caux C - J. Exp. Med. (1997)

D–Lc are more efficient than monocytes in enhancing CD40-induced B cell differentiation. D–Lc, one EBV cell line, and elutriated monocytes were compared, after irradiation, in their ability to enhance (A) cytokine-independent CD40-induced B cell proliferation, (B) cytokine-independent IgG production, and (C) IL-2–dependent IgM production. Increasing numbers of stimulator cells were added to 104 highly purified B cells cultured  over 2.5 × 103 irradiated CD40L L cells. Results are expressed as mean of triplicate cultures (SD ⩽10%). (results from 1 experiment representative of 3).  No significant B cell proliferation and differentiation was observed in absence of CD40L L cells.
© Copyright Policy
Related In: Results  -  Collection

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Figure 5: D–Lc are more efficient than monocytes in enhancing CD40-induced B cell differentiation. D–Lc, one EBV cell line, and elutriated monocytes were compared, after irradiation, in their ability to enhance (A) cytokine-independent CD40-induced B cell proliferation, (B) cytokine-independent IgG production, and (C) IL-2–dependent IgM production. Increasing numbers of stimulator cells were added to 104 highly purified B cells cultured over 2.5 × 103 irradiated CD40L L cells. Results are expressed as mean of triplicate cultures (SD ⩽10%). (results from 1 experiment representative of 3). No significant B cell proliferation and differentiation was observed in absence of CD40L L cells.
Mentions: We compared the effects of various antigen-presenting cells including D–Lc, elutriated monocytes (>90% CD14), and lymphoblastoid cell lines (EBV–LCL) on the proliferation and differentiation of CD40-activated B cells. As shown in Fig. 5 A, monocytes were as potent as D–Lc in enhancing CD40induced B cell proliferation, whereas EBV cell lines were totally inefficient (only one of the three tested is shown in Fig. 5). Significant IgG production, in absence of cytokine, was observed with 3.5 × 102–103 D–Lc, whereas more than 5 × 103 monocytes were required to observe the same effects. At higher cell density (104 cells), monocytes induced levels of IgG in the order of 5 μg/ml compared with 17 μg/ml for D–Lc (Fig. 5 B). Concerning the IL-2–dependent IgM production, a significant effect was observed with less than 450 D–Lc, whereas more than 2.5 × 103 monocytes were required to reach similar levels of IgM secretion. Thus, whereas D–Lc and monocytes shared an equal ability to enhance B cell proliferation, D–Lc are more efficient that monocytes in inducing B cells to secrete IgG and IgM.

Bottom Line: Furthermore, after CD40 ligation, DC enhanced by 30-300-fold the secretion of IgG and IgA by sIgD- B cells (essentially memory B cells).In the presence of DC, naive sIgD+ B cells produced, in response to interleukin-2, large amounts of IgM.Thus, in addition to activating naive T cells in the extrafollicular areas of secondary lymphoid organs, DC may directly modulate B cell growth and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Schering Plough, Laboratory for Immunological Research, Dardilly, France.

ABSTRACT
After antigen capture, dendritic cells (DC) migrate into T cell-rich areas of secondary lymphoid organs, where they induce T cell activation, that subsequently drives B cell activation. Here, we investigate whether DC, generated in vitro, can directly modulate B cell responses, using CD40L-transfected L cells as surrogate activated T cells. DC, through the production of soluble mediators, stimulated by 3- to 6-fold the proliferation and subsequent recovery of B cells. Furthermore, after CD40 ligation, DC enhanced by 30-300-fold the secretion of IgG and IgA by sIgD- B cells (essentially memory B cells). In the presence of DC, naive sIgD+ B cells produced, in response to interleukin-2, large amounts of IgM. Thus, in addition to activating naive T cells in the extrafollicular areas of secondary lymphoid organs, DC may directly modulate B cell growth and differentiation.

Show MeSH
Related in: MedlinePlus