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Follicular dendritic cells specifically express the long CR2/CD21 isoform.

Liu YJ, Xu J, de Bouteiller O, Parham CL, Grouard G, Djossou O, de Saint-Vis B, Lebecque S, Banchereau J, Moore KW - J. Exp. Med. (1997)

Bottom Line: By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated.We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S).Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

View Article: PubMed Central - PubMed

Affiliation: Schering-Plough, Laboratory for Immunological Research, Dardilly, France.

ABSTRACT
This paper describes an antibody (mAb 7D6) that specifically recognizes human follicular dendritic cells (FDCs). By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated. We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S). By screening mouse Ltk- cells transfected with the CD21L cDNA, we further showed that the other two anti-human FDC mAbs DRC-1 and KiM4 also recognize CD21L. Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

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Detection of CD21S and CD21L mRNA by PCR among  CD21++CD14+ FDCs, IgD+CD38− FM, and IgD−CD38+ GC B cells.  The method for FDC isolation is detailed in Materials and Methods and  in Fig. 3. PCR primers used are indicated in the legend for Fig. 2. p7D6  cDNA was used as a positive control.
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Figure 4: Detection of CD21S and CD21L mRNA by PCR among CD21++CD14+ FDCs, IgD+CD38− FM, and IgD−CD38+ GC B cells. The method for FDC isolation is detailed in Materials and Methods and in Fig. 3. PCR primers used are indicated in the legend for Fig. 2. p7D6 cDNA was used as a positive control.

Mentions: The pattern of mAb 7D6 staining suggests that FDC specifically express CD21L, while B cells specifically express CD21S. To directly test this hypothesis, a PCR assay using a 5′ primer starting from basepair 1704 and a 3′ primer starting from basepair 2363 of the short CR2/CD21 sequence, was carried out on RNA from 104 highly purified FDC (Fig. 3) in parallel with follicular mantle B cells (FM) and GC B cells isolated by FACS® sorting. Fig. 4 shows that a single large PCR product was generated from FDC, and a single smaller PCR product was generated from both FM and from GC B cells of the same donor. Further, sequencing analysis of these two PCR products shows that the FDC-derived large PCR product is 836 bp containing the 177-bp insertion that encodes the SCR10a. The B cell–derived PCR product is 659 bp, which does not contain the 177-bp insert (Fig. 5).


Follicular dendritic cells specifically express the long CR2/CD21 isoform.

Liu YJ, Xu J, de Bouteiller O, Parham CL, Grouard G, Djossou O, de Saint-Vis B, Lebecque S, Banchereau J, Moore KW - J. Exp. Med. (1997)

Detection of CD21S and CD21L mRNA by PCR among  CD21++CD14+ FDCs, IgD+CD38− FM, and IgD−CD38+ GC B cells.  The method for FDC isolation is detailed in Materials and Methods and  in Fig. 3. PCR primers used are indicated in the legend for Fig. 2. p7D6  cDNA was used as a positive control.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2196095&req=5

Figure 4: Detection of CD21S and CD21L mRNA by PCR among CD21++CD14+ FDCs, IgD+CD38− FM, and IgD−CD38+ GC B cells. The method for FDC isolation is detailed in Materials and Methods and in Fig. 3. PCR primers used are indicated in the legend for Fig. 2. p7D6 cDNA was used as a positive control.
Mentions: The pattern of mAb 7D6 staining suggests that FDC specifically express CD21L, while B cells specifically express CD21S. To directly test this hypothesis, a PCR assay using a 5′ primer starting from basepair 1704 and a 3′ primer starting from basepair 2363 of the short CR2/CD21 sequence, was carried out on RNA from 104 highly purified FDC (Fig. 3) in parallel with follicular mantle B cells (FM) and GC B cells isolated by FACS® sorting. Fig. 4 shows that a single large PCR product was generated from FDC, and a single smaller PCR product was generated from both FM and from GC B cells of the same donor. Further, sequencing analysis of these two PCR products shows that the FDC-derived large PCR product is 836 bp containing the 177-bp insertion that encodes the SCR10a. The B cell–derived PCR product is 659 bp, which does not contain the 177-bp insert (Fig. 5).

Bottom Line: By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated.We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S).Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

View Article: PubMed Central - PubMed

Affiliation: Schering-Plough, Laboratory for Immunological Research, Dardilly, France.

ABSTRACT
This paper describes an antibody (mAb 7D6) that specifically recognizes human follicular dendritic cells (FDCs). By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated. We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S). By screening mouse Ltk- cells transfected with the CD21L cDNA, we further showed that the other two anti-human FDC mAbs DRC-1 and KiM4 also recognize CD21L. Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

Show MeSH