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Follicular dendritic cells specifically express the long CR2/CD21 isoform.

Liu YJ, Xu J, de Bouteiller O, Parham CL, Grouard G, Djossou O, de Saint-Vis B, Lebecque S, Banchereau J, Moore KW - J. Exp. Med. (1997)

Bottom Line: By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated.We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S).Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

View Article: PubMed Central - PubMed

Affiliation: Schering-Plough, Laboratory for Immunological Research, Dardilly, France.

ABSTRACT
This paper describes an antibody (mAb 7D6) that specifically recognizes human follicular dendritic cells (FDCs). By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated. We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S). By screening mouse Ltk- cells transfected with the CD21L cDNA, we further showed that the other two anti-human FDC mAbs DRC-1 and KiM4 also recognize CD21L. Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

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Isolation of highly purified FDC by FACS® sorting. (A) Low  density tonsillar cells were stained by anti–CD21-PE and anti–CD14FITC (detailed in Materials and Methods). FDCs were sorted according  to their CD21++CD14+ phenotype. B cells and monocytes could be recognized as CD21+CD14− and CD21−CD14+ cells, respectively. (B and  C) Giemsa staining of FACS® sorted FDC (×400 and ×1,000).
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Figure 3: Isolation of highly purified FDC by FACS® sorting. (A) Low density tonsillar cells were stained by anti–CD21-PE and anti–CD14FITC (detailed in Materials and Methods). FDCs were sorted according to their CD21++CD14+ phenotype. B cells and monocytes could be recognized as CD21+CD14− and CD21−CD14+ cells, respectively. (B and C) Giemsa staining of FACS® sorted FDC (×400 and ×1,000).

Mentions: Since human B cells, T cells, fibroblasts, endothelial cells, and epithelial cells express no or low levels of CD14, and human T cells, fibroblasts, endothelial cells, and epithelial cells express no or low levels of CD21, CD14highCD21high FDC were isolated by FACS® sorting of enriched FDC preparations by Percoll gradient. After cell sorting, the resulting population contained >98% pure single FDC (Fig. 3). These highly purified FDCs may have been damaged inasmuch as they displayed cytoplasm losses and were unable to support B cell growth in vitro. However, these cells were used for PCR assays.


Follicular dendritic cells specifically express the long CR2/CD21 isoform.

Liu YJ, Xu J, de Bouteiller O, Parham CL, Grouard G, Djossou O, de Saint-Vis B, Lebecque S, Banchereau J, Moore KW - J. Exp. Med. (1997)

Isolation of highly purified FDC by FACS® sorting. (A) Low  density tonsillar cells were stained by anti–CD21-PE and anti–CD14FITC (detailed in Materials and Methods). FDCs were sorted according  to their CD21++CD14+ phenotype. B cells and monocytes could be recognized as CD21+CD14− and CD21−CD14+ cells, respectively. (B and  C) Giemsa staining of FACS® sorted FDC (×400 and ×1,000).
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Related In: Results  -  Collection

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Figure 3: Isolation of highly purified FDC by FACS® sorting. (A) Low density tonsillar cells were stained by anti–CD21-PE and anti–CD14FITC (detailed in Materials and Methods). FDCs were sorted according to their CD21++CD14+ phenotype. B cells and monocytes could be recognized as CD21+CD14− and CD21−CD14+ cells, respectively. (B and C) Giemsa staining of FACS® sorted FDC (×400 and ×1,000).
Mentions: Since human B cells, T cells, fibroblasts, endothelial cells, and epithelial cells express no or low levels of CD14, and human T cells, fibroblasts, endothelial cells, and epithelial cells express no or low levels of CD21, CD14highCD21high FDC were isolated by FACS® sorting of enriched FDC preparations by Percoll gradient. After cell sorting, the resulting population contained >98% pure single FDC (Fig. 3). These highly purified FDCs may have been damaged inasmuch as they displayed cytoplasm losses and were unable to support B cell growth in vitro. However, these cells were used for PCR assays.

Bottom Line: By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated.We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S).Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

View Article: PubMed Central - PubMed

Affiliation: Schering-Plough, Laboratory for Immunological Research, Dardilly, France.

ABSTRACT
This paper describes an antibody (mAb 7D6) that specifically recognizes human follicular dendritic cells (FDCs). By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated. We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S). By screening mouse Ltk- cells transfected with the CD21L cDNA, we further showed that the other two anti-human FDC mAbs DRC-1 and KiM4 also recognize CD21L. Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

Show MeSH