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Follicular dendritic cells specifically express the long CR2/CD21 isoform.

Liu YJ, Xu J, de Bouteiller O, Parham CL, Grouard G, Djossou O, de Saint-Vis B, Lebecque S, Banchereau J, Moore KW - J. Exp. Med. (1997)

Bottom Line: By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated.We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S).Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

View Article: PubMed Central - PubMed

Affiliation: Schering-Plough, Laboratory for Immunological Research, Dardilly, France.

ABSTRACT
This paper describes an antibody (mAb 7D6) that specifically recognizes human follicular dendritic cells (FDCs). By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated. We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S). By screening mouse Ltk- cells transfected with the CD21L cDNA, we further showed that the other two anti-human FDC mAbs DRC-1 and KiM4 also recognize CD21L. Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

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mAb 7D6 specifically stains FDC networks within  tonsillar and splenic GCs or  FDCs in isolated form. (A) Red  mAb 7D6 staining of FDC networks within a GC of human  tonsil (DZ, dark zone; LZ, light  zone; ×200). (B) Red antiCD21 staining of FDC networks  and B lymphocytes within the  follicular mantle (FM) and extrafollicular area (A and B show  the same secondary follicle on  two serial sections). (C) mAb  7D6 staining of FDC networks  within a primary follicle of human spleen (CA, central arteriole; ×200). (D) anti-CD21  staining of FDC networks as well  as follicular B cells and marginal  zone (MZ) B cells (C and D  show the same splenic white  pulp on two serial sections). (E)  Negative mAb 7D6 staining on  human thymus (×200). (F) Positive staining of anti-ICAM1/ CD54 on human thymus (E and  F show the same thymic area on  two serial sections) (C, cortex.  M, medullar). (G) Giemsa staining of isolated FDC-lymphocyte  clusters. FDC can be recognized  as cells containing one or two  big round nuclei with decondensed chromatin and clear nucleoli (×1000). (H) mAb 7D6  staining of isolated FDCs.
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Figure 1: mAb 7D6 specifically stains FDC networks within tonsillar and splenic GCs or FDCs in isolated form. (A) Red mAb 7D6 staining of FDC networks within a GC of human tonsil (DZ, dark zone; LZ, light zone; ×200). (B) Red antiCD21 staining of FDC networks and B lymphocytes within the follicular mantle (FM) and extrafollicular area (A and B show the same secondary follicle on two serial sections). (C) mAb 7D6 staining of FDC networks within a primary follicle of human spleen (CA, central arteriole; ×200). (D) anti-CD21 staining of FDC networks as well as follicular B cells and marginal zone (MZ) B cells (C and D show the same splenic white pulp on two serial sections). (E) Negative mAb 7D6 staining on human thymus (×200). (F) Positive staining of anti-ICAM1/ CD54 on human thymus (E and F show the same thymic area on two serial sections) (C, cortex. M, medullar). (G) Giemsa staining of isolated FDC-lymphocyte clusters. FDC can be recognized as cells containing one or two big round nuclei with decondensed chromatin and clear nucleoli (×1000). (H) mAb 7D6 staining of isolated FDCs.

Mentions: mAb 7D6 was selected because it specifically stains FDC networks on tonsillar and splenic sections (Fig. 1, A and C). The reactivity on FDC networks was further confirmed by staining of isolated FDCs (Fig. 1, G and H). 7D6 antibody did not give any specific staining on sections from fetal thymus (Fig. 1 E) or fetal liver (not shown). There was no positive staining of mAb 7D6 on total cell suspensions of bone marrow and peripheral blood by FACS® analysis (not shown).


Follicular dendritic cells specifically express the long CR2/CD21 isoform.

Liu YJ, Xu J, de Bouteiller O, Parham CL, Grouard G, Djossou O, de Saint-Vis B, Lebecque S, Banchereau J, Moore KW - J. Exp. Med. (1997)

mAb 7D6 specifically stains FDC networks within  tonsillar and splenic GCs or  FDCs in isolated form. (A) Red  mAb 7D6 staining of FDC networks within a GC of human  tonsil (DZ, dark zone; LZ, light  zone; ×200). (B) Red antiCD21 staining of FDC networks  and B lymphocytes within the  follicular mantle (FM) and extrafollicular area (A and B show  the same secondary follicle on  two serial sections). (C) mAb  7D6 staining of FDC networks  within a primary follicle of human spleen (CA, central arteriole; ×200). (D) anti-CD21  staining of FDC networks as well  as follicular B cells and marginal  zone (MZ) B cells (C and D  show the same splenic white  pulp on two serial sections). (E)  Negative mAb 7D6 staining on  human thymus (×200). (F) Positive staining of anti-ICAM1/ CD54 on human thymus (E and  F show the same thymic area on  two serial sections) (C, cortex.  M, medullar). (G) Giemsa staining of isolated FDC-lymphocyte  clusters. FDC can be recognized  as cells containing one or two  big round nuclei with decondensed chromatin and clear nucleoli (×1000). (H) mAb 7D6  staining of isolated FDCs.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2196095&req=5

Figure 1: mAb 7D6 specifically stains FDC networks within tonsillar and splenic GCs or FDCs in isolated form. (A) Red mAb 7D6 staining of FDC networks within a GC of human tonsil (DZ, dark zone; LZ, light zone; ×200). (B) Red antiCD21 staining of FDC networks and B lymphocytes within the follicular mantle (FM) and extrafollicular area (A and B show the same secondary follicle on two serial sections). (C) mAb 7D6 staining of FDC networks within a primary follicle of human spleen (CA, central arteriole; ×200). (D) anti-CD21 staining of FDC networks as well as follicular B cells and marginal zone (MZ) B cells (C and D show the same splenic white pulp on two serial sections). (E) Negative mAb 7D6 staining on human thymus (×200). (F) Positive staining of anti-ICAM1/ CD54 on human thymus (E and F show the same thymic area on two serial sections) (C, cortex. M, medullar). (G) Giemsa staining of isolated FDC-lymphocyte clusters. FDC can be recognized as cells containing one or two big round nuclei with decondensed chromatin and clear nucleoli (×1000). (H) mAb 7D6 staining of isolated FDCs.
Mentions: mAb 7D6 was selected because it specifically stains FDC networks on tonsillar and splenic sections (Fig. 1, A and C). The reactivity on FDC networks was further confirmed by staining of isolated FDCs (Fig. 1, G and H). 7D6 antibody did not give any specific staining on sections from fetal thymus (Fig. 1 E) or fetal liver (not shown). There was no positive staining of mAb 7D6 on total cell suspensions of bone marrow and peripheral blood by FACS® analysis (not shown).

Bottom Line: By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated.We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S).Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

View Article: PubMed Central - PubMed

Affiliation: Schering-Plough, Laboratory for Immunological Research, Dardilly, France.

ABSTRACT
This paper describes an antibody (mAb 7D6) that specifically recognizes human follicular dendritic cells (FDCs). By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated. We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S). By screening mouse Ltk- cells transfected with the CD21L cDNA, we further showed that the other two anti-human FDC mAbs DRC-1 and KiM4 also recognize CD21L. Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.

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