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CD1-mediated gamma/delta T cell maturation of dendritic cells.

Leslie DS, Vincent MS, Spada FM, Das H, Sugita M, Morita CT, Brenner MB - J. Exp. Med. (2002)

Bottom Line: In addition, these DCs were able to efficiently present peptide antigens to naive CD4+ T cells.CD1-restricted gamma/delta T cell recognition of immature DCs provides the human immune system with the capacity to rapidly generate a pool of mature DCs early during microbial invasion.This may be an important source of critical host signals for T helper type 1 polarization of antigen-specific naive T cells and the subsequent adaptive immune response.

View Article: PubMed Central - PubMed

Affiliation: Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women's Hospital at Harvard Medical School, Boston, MA 02115, USA.

ABSTRACT
Immature myeloid dendritic cells (DCs) express only low levels of major histocompatibility complex (MHC) class II but express high levels of CD1 a, b, and c antigen-presenting molecules at the cell surface. As Vdelta1+ gamma/delta T cells are the main tissue subset of gamma/delta T cells and they are known to recognize CD1c in the absence of specific foreign antigen recognition, we examined the possible interaction of these T cells with immature DCs. We show that CD1-restricted gamma/delta T cells can mediate the maturation of DCs. DC maturation required cell-cell contact and could be blocked by antibodies against CD1c. The maturation process was partially mediated by tumor necrosis factor alpha. Importantly, immature DCs matured in the presence of lipopolysaccharide and CD1-restricted gamma/delta T cells produced bioactive interleukin-12p70. In addition, these DCs were able to efficiently present peptide antigens to naive CD4+ T cells. CD1-restricted gamma/delta T cell recognition of immature DCs provides the human immune system with the capacity to rapidly generate a pool of mature DCs early during microbial invasion. This may be an important source of critical host signals for T helper type 1 polarization of antigen-specific naive T cells and the subsequent adaptive immune response.

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Related in: MedlinePlus

γ/δ T cell–induced DC maturation is mediated by CD1c. Immature monocyte-derived DCs were cultured with the CD1c-restricted γ/δ clone JR.2 at a T cell:DC ratio of 1:9 for 48 h in culture medium or in the presence of anti-CD1 mAb blocking. Treatment with anti-CD1c mAb F10/21A3 inhibited up-regulation of CD83 and CD86 by 64 and 78%, respectively. Blocking with mAb directed against CD1a (OKT6) did not inhibit maturation. These results are representative of three independent experiments using different DC donors.
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fig2: γ/δ T cell–induced DC maturation is mediated by CD1c. Immature monocyte-derived DCs were cultured with the CD1c-restricted γ/δ clone JR.2 at a T cell:DC ratio of 1:9 for 48 h in culture medium or in the presence of anti-CD1 mAb blocking. Treatment with anti-CD1c mAb F10/21A3 inhibited up-regulation of CD83 and CD86 by 64 and 78%, respectively. Blocking with mAb directed against CD1a (OKT6) did not inhibit maturation. These results are representative of three independent experiments using different DC donors.

Mentions: As only certain γ/δ T cells were able to induce the maturation of DCs, we wished to determine if recognition of CD1c by these T cells was critical for inducing DC maturation. During a 48-h coculture of γ/δ clone JR.2 with immature DCs at a T cell:DC ratio of 1:9, treatment with anti-CD1c mAb F10/21A3 inhibited up-regulation of CD83 and CD86 by 64 and 78%, respectively, when compared with DCs cultured in medium alone. Treatment with a mAb directed against CD1a (OKT6) had no such effect (Fig. 2) . These data indicate that CD1c recognition by certain γ/δ T cells is required for the ability to induce in vitro monocyte-derived DC maturation.


CD1-mediated gamma/delta T cell maturation of dendritic cells.

Leslie DS, Vincent MS, Spada FM, Das H, Sugita M, Morita CT, Brenner MB - J. Exp. Med. (2002)

γ/δ T cell–induced DC maturation is mediated by CD1c. Immature monocyte-derived DCs were cultured with the CD1c-restricted γ/δ clone JR.2 at a T cell:DC ratio of 1:9 for 48 h in culture medium or in the presence of anti-CD1 mAb blocking. Treatment with anti-CD1c mAb F10/21A3 inhibited up-regulation of CD83 and CD86 by 64 and 78%, respectively. Blocking with mAb directed against CD1a (OKT6) did not inhibit maturation. These results are representative of three independent experiments using different DC donors.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2196072&req=5

fig2: γ/δ T cell–induced DC maturation is mediated by CD1c. Immature monocyte-derived DCs were cultured with the CD1c-restricted γ/δ clone JR.2 at a T cell:DC ratio of 1:9 for 48 h in culture medium or in the presence of anti-CD1 mAb blocking. Treatment with anti-CD1c mAb F10/21A3 inhibited up-regulation of CD83 and CD86 by 64 and 78%, respectively. Blocking with mAb directed against CD1a (OKT6) did not inhibit maturation. These results are representative of three independent experiments using different DC donors.
Mentions: As only certain γ/δ T cells were able to induce the maturation of DCs, we wished to determine if recognition of CD1c by these T cells was critical for inducing DC maturation. During a 48-h coculture of γ/δ clone JR.2 with immature DCs at a T cell:DC ratio of 1:9, treatment with anti-CD1c mAb F10/21A3 inhibited up-regulation of CD83 and CD86 by 64 and 78%, respectively, when compared with DCs cultured in medium alone. Treatment with a mAb directed against CD1a (OKT6) had no such effect (Fig. 2) . These data indicate that CD1c recognition by certain γ/δ T cells is required for the ability to induce in vitro monocyte-derived DC maturation.

Bottom Line: In addition, these DCs were able to efficiently present peptide antigens to naive CD4+ T cells.CD1-restricted gamma/delta T cell recognition of immature DCs provides the human immune system with the capacity to rapidly generate a pool of mature DCs early during microbial invasion.This may be an important source of critical host signals for T helper type 1 polarization of antigen-specific naive T cells and the subsequent adaptive immune response.

View Article: PubMed Central - PubMed

Affiliation: Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women's Hospital at Harvard Medical School, Boston, MA 02115, USA.

ABSTRACT
Immature myeloid dendritic cells (DCs) express only low levels of major histocompatibility complex (MHC) class II but express high levels of CD1 a, b, and c antigen-presenting molecules at the cell surface. As Vdelta1+ gamma/delta T cells are the main tissue subset of gamma/delta T cells and they are known to recognize CD1c in the absence of specific foreign antigen recognition, we examined the possible interaction of these T cells with immature DCs. We show that CD1-restricted gamma/delta T cells can mediate the maturation of DCs. DC maturation required cell-cell contact and could be blocked by antibodies against CD1c. The maturation process was partially mediated by tumor necrosis factor alpha. Importantly, immature DCs matured in the presence of lipopolysaccharide and CD1-restricted gamma/delta T cells produced bioactive interleukin-12p70. In addition, these DCs were able to efficiently present peptide antigens to naive CD4+ T cells. CD1-restricted gamma/delta T cell recognition of immature DCs provides the human immune system with the capacity to rapidly generate a pool of mature DCs early during microbial invasion. This may be an important source of critical host signals for T helper type 1 polarization of antigen-specific naive T cells and the subsequent adaptive immune response.

Show MeSH
Related in: MedlinePlus