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Viral superantigen drives extrafollicular and follicular B cell differentiation leading to virus-specific antibody production.

Luther SA, Gulbranson-Judge A, Acha-Orbea H, MacLennan IC - J. Exp. Med. (1997)

Bottom Line: The response to MMTV(SW) in draining lymph nodes was compared with the response to haptenated chicken gamma globulin (NP-CGG) using flow cytometry and immunohistology.Germinal centers develop in both responses, but those induced by MMTV(SW) appear later and are smaller.Most T cells activated in the T zone and germinal centers in the MMTV(SW) response are superantigen specific and these persist for weeks in lymph nodes draining the site MMTV(SW) injection: this contrasts with the selective loss of superantigen-specific T cells from other secondary lymphoid tissues.

View Article: PubMed Central - PubMed

Affiliation: Ludwig Institute for Cancer Research, University of Lansanne, Epalinges.

ABSTRACT
Mouse mammary tumor virus (MMTV[SW]) encodes a superantigen expressed by infected B cells. It evokes an antibody response specific for viral envelope protein, indicating selective activation of antigen-specific B cells. The response to MMTV(SW) in draining lymph nodes was compared with the response to haptenated chicken gamma globulin (NP-CGG) using flow cytometry and immunohistology. T cell priming occurs in both responses, with T cells proliferating in association with interdigitating dendritic cells in the T zone. T cell proliferation continues in the presence of B cells in the outer T zone, and B blasts then undergo exponential growth and differentiation into plasma cells in the medullary cords. Germinal centers develop in both responses, but those induced by MMTV(SW) appear later and are smaller. Most T cells activated in the T zone and germinal centers in the MMTV(SW) response are superantigen specific and these persist for weeks in lymph nodes draining the site MMTV(SW) injection: this contrasts with the selective loss of superantigen-specific T cells from other secondary lymphoid tissues. The results indicate that this viral superantigen, when expressed by professional antigen-presenting cells, drives extrafollicular and follicular B cell differentiation leading to virus-specific antibody production.

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Summary of the  major events of the lymph node  immune response to either  MMTV(SW) superantigen or to  NPCGG. This sketch summarizes the responses that were  quantified by flow cytometry  (Figs. 2 and 4) and localized by  immunocytochemistry. (Figs. 3,  5–7). Emphasis is put on the sites  of T and B cell activation and  differentiation with symbols representing the relative number of  cells in a given compartment.
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Figure 8: Summary of the major events of the lymph node immune response to either MMTV(SW) superantigen or to NPCGG. This sketch summarizes the responses that were quantified by flow cytometry (Figs. 2 and 4) and localized by immunocytochemistry. (Figs. 3, 5–7). Emphasis is put on the sites of T and B cell activation and differentiation with symbols representing the relative number of cells in a given compartment.

Mentions: The lymphoid architecture involved in specific immune responses has been investigated mainly using model antigens and rarely with infectious agents. Here, we compared the histological appearance of lymph nodes after subcutaneous injection of a T-dependent haptenated protein (NPCGG) with a murine retrovirus (MMTV) that expresses a SAg soon after infection. We show that the extrafollicular T and B cell activation during both responses are remarkably similar. Both antigens induced germinal centers, although these were smaller and appeared later in the response to MMTV(SW). The sequence of the responses to these two antigens are summarized diagrammatically in Fig. 8.


Viral superantigen drives extrafollicular and follicular B cell differentiation leading to virus-specific antibody production.

Luther SA, Gulbranson-Judge A, Acha-Orbea H, MacLennan IC - J. Exp. Med. (1997)

Summary of the  major events of the lymph node  immune response to either  MMTV(SW) superantigen or to  NPCGG. This sketch summarizes the responses that were  quantified by flow cytometry  (Figs. 2 and 4) and localized by  immunocytochemistry. (Figs. 3,  5–7). Emphasis is put on the sites  of T and B cell activation and  differentiation with symbols representing the relative number of  cells in a given compartment.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2196032&req=5

Figure 8: Summary of the major events of the lymph node immune response to either MMTV(SW) superantigen or to NPCGG. This sketch summarizes the responses that were quantified by flow cytometry (Figs. 2 and 4) and localized by immunocytochemistry. (Figs. 3, 5–7). Emphasis is put on the sites of T and B cell activation and differentiation with symbols representing the relative number of cells in a given compartment.
Mentions: The lymphoid architecture involved in specific immune responses has been investigated mainly using model antigens and rarely with infectious agents. Here, we compared the histological appearance of lymph nodes after subcutaneous injection of a T-dependent haptenated protein (NPCGG) with a murine retrovirus (MMTV) that expresses a SAg soon after infection. We show that the extrafollicular T and B cell activation during both responses are remarkably similar. Both antigens induced germinal centers, although these were smaller and appeared later in the response to MMTV(SW). The sequence of the responses to these two antigens are summarized diagrammatically in Fig. 8.

Bottom Line: The response to MMTV(SW) in draining lymph nodes was compared with the response to haptenated chicken gamma globulin (NP-CGG) using flow cytometry and immunohistology.Germinal centers develop in both responses, but those induced by MMTV(SW) appear later and are smaller.Most T cells activated in the T zone and germinal centers in the MMTV(SW) response are superantigen specific and these persist for weeks in lymph nodes draining the site MMTV(SW) injection: this contrasts with the selective loss of superantigen-specific T cells from other secondary lymphoid tissues.

View Article: PubMed Central - PubMed

Affiliation: Ludwig Institute for Cancer Research, University of Lansanne, Epalinges.

ABSTRACT
Mouse mammary tumor virus (MMTV[SW]) encodes a superantigen expressed by infected B cells. It evokes an antibody response specific for viral envelope protein, indicating selective activation of antigen-specific B cells. The response to MMTV(SW) in draining lymph nodes was compared with the response to haptenated chicken gamma globulin (NP-CGG) using flow cytometry and immunohistology. T cell priming occurs in both responses, with T cells proliferating in association with interdigitating dendritic cells in the T zone. T cell proliferation continues in the presence of B cells in the outer T zone, and B blasts then undergo exponential growth and differentiation into plasma cells in the medullary cords. Germinal centers develop in both responses, but those induced by MMTV(SW) appear later and are smaller. Most T cells activated in the T zone and germinal centers in the MMTV(SW) response are superantigen specific and these persist for weeks in lymph nodes draining the site MMTV(SW) injection: this contrasts with the selective loss of superantigen-specific T cells from other secondary lymphoid tissues. The results indicate that this viral superantigen, when expressed by professional antigen-presenting cells, drives extrafollicular and follicular B cell differentiation leading to virus-specific antibody production.

Show MeSH
Related in: MedlinePlus