Limits...
The absence of interleukin 9 does not affect the development of allergen-induced pulmonary inflammation nor airway hyperreactivity.

McMillan SJ, Bishop B, Townsend MJ, McKenzie AN, Lloyd CM - J. Exp. Med. (2002)

Bottom Line: Interleukin (IL)-9 is a pleiotropic cytokine secreted by T helper (Th)2 cells and has been proposed as a candidate gene for asthma and allergy.Goblet cell hyperplasia and immunoglobulin (Ig) E production were also unaffected by the lack of IL-9.These findings indicate that IL-9 is not obligatory for the development of eosinophilia and AHR, and imply that other Th2 cytokines can act in a compensatory fashion.

View Article: PubMed Central - PubMed

Affiliation: Leukocyte Biology Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College of Science, Technology, and Medicine, London SW7 2AZ, England.

ABSTRACT
Interleukin (IL)-9 is a pleiotropic cytokine secreted by T helper (Th)2 cells and has been proposed as a candidate gene for asthma and allergy. We have used mice genetically deficient in IL-9 to determine the role of this cytokine in the pathophysiologic features of the allergic pulmonary response-airway hyperreactivity (AHR) and eosinophilia. We have demonstrated that IL-9 is not required for the development of a robust Th2 response to allergen in sensitized mice. IL-9 knockout mice developed a similar degree of eosinophilic inflammation and AHR to their wild-type littermates. Goblet cell hyperplasia and immunoglobulin (Ig) E production were also unaffected by the lack of IL-9. Moreover, levels of bronchoalveolar lavage (BAL) IL-4, IL-5, and IL-13 were comparable between wild-type and knockout mice. These findings indicate that IL-9 is not obligatory for the development of eosinophilia and AHR, and imply that other Th2 cytokines can act in a compensatory fashion.

Show MeSH

Related in: MedlinePlus

Differential cell counts in BAL (A) and lung tissue digest (B) from WT mice (white bars) and IL-9 KO mice (black bars). Mice were killed 24 h after the final OVA challenge. BAL and lung tissue digest cells were isolated as described in Materials and Methods. Values are expressed as mean ± SEM; n = 11–14 per group in two separate experiments.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2196020&req=5

fig2: Differential cell counts in BAL (A) and lung tissue digest (B) from WT mice (white bars) and IL-9 KO mice (black bars). Mice were killed 24 h after the final OVA challenge. BAL and lung tissue digest cells were isolated as described in Materials and Methods. Values are expressed as mean ± SEM; n = 11–14 per group in two separate experiments.

Mentions: Pulmonary inflammation is one of the characteristic hallmarks of the allergic response to allergen. Leukocytic infiltrates are found within the airway lumen, as observed in BAL, and in the lung interstitium as observed in histological sections. The predominant leukocyte is the eosinophil in conjunction with lymphocytes, primarily of the Th2 phenotype. We determined the extent of inflammation in both compartments of the lung in sensitized WT and IL-9 KO mice after serial OVA challenge. Cell recruitment to BAL and lung was comparable between WT KO mice (Fig. 2 A and B). Differential counts revealed that infiltrates in both strains of mice were composed of mainly eosinophils and lymphocytes. However, there was no difference in either the total leukocyte infiltration of each compartment or in numbers of any leukocyte subset between IL-9–deficient and WT mice.


The absence of interleukin 9 does not affect the development of allergen-induced pulmonary inflammation nor airway hyperreactivity.

McMillan SJ, Bishop B, Townsend MJ, McKenzie AN, Lloyd CM - J. Exp. Med. (2002)

Differential cell counts in BAL (A) and lung tissue digest (B) from WT mice (white bars) and IL-9 KO mice (black bars). Mice were killed 24 h after the final OVA challenge. BAL and lung tissue digest cells were isolated as described in Materials and Methods. Values are expressed as mean ± SEM; n = 11–14 per group in two separate experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2196020&req=5

fig2: Differential cell counts in BAL (A) and lung tissue digest (B) from WT mice (white bars) and IL-9 KO mice (black bars). Mice were killed 24 h after the final OVA challenge. BAL and lung tissue digest cells were isolated as described in Materials and Methods. Values are expressed as mean ± SEM; n = 11–14 per group in two separate experiments.
Mentions: Pulmonary inflammation is one of the characteristic hallmarks of the allergic response to allergen. Leukocytic infiltrates are found within the airway lumen, as observed in BAL, and in the lung interstitium as observed in histological sections. The predominant leukocyte is the eosinophil in conjunction with lymphocytes, primarily of the Th2 phenotype. We determined the extent of inflammation in both compartments of the lung in sensitized WT and IL-9 KO mice after serial OVA challenge. Cell recruitment to BAL and lung was comparable between WT KO mice (Fig. 2 A and B). Differential counts revealed that infiltrates in both strains of mice were composed of mainly eosinophils and lymphocytes. However, there was no difference in either the total leukocyte infiltration of each compartment or in numbers of any leukocyte subset between IL-9–deficient and WT mice.

Bottom Line: Interleukin (IL)-9 is a pleiotropic cytokine secreted by T helper (Th)2 cells and has been proposed as a candidate gene for asthma and allergy.Goblet cell hyperplasia and immunoglobulin (Ig) E production were also unaffected by the lack of IL-9.These findings indicate that IL-9 is not obligatory for the development of eosinophilia and AHR, and imply that other Th2 cytokines can act in a compensatory fashion.

View Article: PubMed Central - PubMed

Affiliation: Leukocyte Biology Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College of Science, Technology, and Medicine, London SW7 2AZ, England.

ABSTRACT
Interleukin (IL)-9 is a pleiotropic cytokine secreted by T helper (Th)2 cells and has been proposed as a candidate gene for asthma and allergy. We have used mice genetically deficient in IL-9 to determine the role of this cytokine in the pathophysiologic features of the allergic pulmonary response-airway hyperreactivity (AHR) and eosinophilia. We have demonstrated that IL-9 is not required for the development of a robust Th2 response to allergen in sensitized mice. IL-9 knockout mice developed a similar degree of eosinophilic inflammation and AHR to their wild-type littermates. Goblet cell hyperplasia and immunoglobulin (Ig) E production were also unaffected by the lack of IL-9. Moreover, levels of bronchoalveolar lavage (BAL) IL-4, IL-5, and IL-13 were comparable between wild-type and knockout mice. These findings indicate that IL-9 is not obligatory for the development of eosinophilia and AHR, and imply that other Th2 cytokines can act in a compensatory fashion.

Show MeSH
Related in: MedlinePlus