Limits...
Osteopontin is involved in the initiation of cutaneous contact hypersensitivity by inducing Langerhans and dendritic cell migration to lymph nodes.

Weiss JM, Renkl AC, Maier CS, Kimmig M, Liaw L, Ahrens T, Kon S, Maeda M, Hotta H, Uede T, Simon JC - J. Exp. Med. (2001)

Bottom Line: Characterizing OPN function for LC/DC migration we found upregulated OPN expression in hapten sensitized skin and draining lymph nodes.OPN induces chemotactic LC/DC migration, initiates their emigration from the epidermis, and attracts LCs/DCs to draining lymph nodes by interacting with CD44 and alphav integrin.In conclusion, OPN is an important factor in the initiation of CHS by guiding LCs/DCs from skin into lymphatic organs.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, University of Freiburg, D-79104 Freiburg, Germany. weiss@haut.ukl.uni-freiburg.de

ABSTRACT
Osteopontin (OPN) is a chemotactic protein that attracts immune cells, to inflammatory sites. The sensitization phase of allergic cutaneous contact hypersensitivity (CHS) requires the migration of Langerhans cells/dendritic cells (LCs/DCs) from skin to draining lymph nodes. Characterizing OPN function for LC/DC migration we found upregulated OPN expression in hapten sensitized skin and draining lymph nodes. OPN induces chemotactic LC/DC migration, initiates their emigration from the epidermis, and attracts LCs/DCs to draining lymph nodes by interacting with CD44 and alphav integrin. Furthermore, OPN-deficient mice have a significantly reduced CHS response that correlates with an impaired ability of OPN-deficient mice to attract LCs/DCs to draining lymph nodes. In conclusion, OPN is an important factor in the initiation of CHS by guiding LCs/DCs from skin into lymphatic organs.

Show MeSH

Related in: MedlinePlus

OPN-deficient mice have a reduced CHS-response to TNCB. In OPN  mutant mice and wild-type litter mates CHS was induced as described in Materials and Methods. *Statistically significant reduction of ear swelling at P < 0.001 (one way ANOVA, Dunnett's test). Each bar represents mean ear swelling data pooled from four animals measured 24 and 48 h after challenge. Representative of three independent experiments is shown.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2195976&req=5

fig7: OPN-deficient mice have a reduced CHS-response to TNCB. In OPN mutant mice and wild-type litter mates CHS was induced as described in Materials and Methods. *Statistically significant reduction of ear swelling at P < 0.001 (one way ANOVA, Dunnett's test). Each bar represents mean ear swelling data pooled from four animals measured 24 and 48 h after challenge. Representative of three independent experiments is shown.

Mentions: To finally test the role of OPN in vivo we investigated the CHS response of mice lacking a functional OPN gene (28). OPN-deficient C57BL/6 mice and wild-type littermates were sensitized epicutaneously to the hapten TNCB on day 0. Upon challenge with TNCB at the ear on day 5, wild-type mice showed a normal ear swelling response (Fig. 7) . However, when the ears of TNCB sensitized OPN-deficient littermates (−/−) were challenged with the hapten, these mice had a significantly reduced ear swelling response 24 and 48 h after challenge (Fig. 7). Based on these findings we postulated that OPN is of key importance for the capacity of LCs to induce a CHS response against epicutaneously applied haptens.


Osteopontin is involved in the initiation of cutaneous contact hypersensitivity by inducing Langerhans and dendritic cell migration to lymph nodes.

Weiss JM, Renkl AC, Maier CS, Kimmig M, Liaw L, Ahrens T, Kon S, Maeda M, Hotta H, Uede T, Simon JC - J. Exp. Med. (2001)

OPN-deficient mice have a reduced CHS-response to TNCB. In OPN  mutant mice and wild-type litter mates CHS was induced as described in Materials and Methods. *Statistically significant reduction of ear swelling at P < 0.001 (one way ANOVA, Dunnett's test). Each bar represents mean ear swelling data pooled from four animals measured 24 and 48 h after challenge. Representative of three independent experiments is shown.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2195976&req=5

fig7: OPN-deficient mice have a reduced CHS-response to TNCB. In OPN mutant mice and wild-type litter mates CHS was induced as described in Materials and Methods. *Statistically significant reduction of ear swelling at P < 0.001 (one way ANOVA, Dunnett's test). Each bar represents mean ear swelling data pooled from four animals measured 24 and 48 h after challenge. Representative of three independent experiments is shown.
Mentions: To finally test the role of OPN in vivo we investigated the CHS response of mice lacking a functional OPN gene (28). OPN-deficient C57BL/6 mice and wild-type littermates were sensitized epicutaneously to the hapten TNCB on day 0. Upon challenge with TNCB at the ear on day 5, wild-type mice showed a normal ear swelling response (Fig. 7) . However, when the ears of TNCB sensitized OPN-deficient littermates (−/−) were challenged with the hapten, these mice had a significantly reduced ear swelling response 24 and 48 h after challenge (Fig. 7). Based on these findings we postulated that OPN is of key importance for the capacity of LCs to induce a CHS response against epicutaneously applied haptens.

Bottom Line: Characterizing OPN function for LC/DC migration we found upregulated OPN expression in hapten sensitized skin and draining lymph nodes.OPN induces chemotactic LC/DC migration, initiates their emigration from the epidermis, and attracts LCs/DCs to draining lymph nodes by interacting with CD44 and alphav integrin.In conclusion, OPN is an important factor in the initiation of CHS by guiding LCs/DCs from skin into lymphatic organs.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, University of Freiburg, D-79104 Freiburg, Germany. weiss@haut.ukl.uni-freiburg.de

ABSTRACT
Osteopontin (OPN) is a chemotactic protein that attracts immune cells, to inflammatory sites. The sensitization phase of allergic cutaneous contact hypersensitivity (CHS) requires the migration of Langerhans cells/dendritic cells (LCs/DCs) from skin to draining lymph nodes. Characterizing OPN function for LC/DC migration we found upregulated OPN expression in hapten sensitized skin and draining lymph nodes. OPN induces chemotactic LC/DC migration, initiates their emigration from the epidermis, and attracts LCs/DCs to draining lymph nodes by interacting with CD44 and alphav integrin. Furthermore, OPN-deficient mice have a significantly reduced CHS response that correlates with an impaired ability of OPN-deficient mice to attract LCs/DCs to draining lymph nodes. In conclusion, OPN is an important factor in the initiation of CHS by guiding LCs/DCs from skin into lymphatic organs.

Show MeSH
Related in: MedlinePlus