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A member of the dendritic cell family that enters B cell follicles and stimulates primary antibody responses identified by a mannose receptor fusion protein.

Berney C, Herren S, Power CA, Gordon S, Martinez-Pomares L, Kosco-Vilbois MH - J. Exp. Med. (1999)

Bottom Line: Dendritic cells (DCs) are known to activate naive T cells to become effective helper cells.Furthermore, they produced significantly more of the lymphocyte chemoattractant, macrophage inflammatory protein (MIP)-1alpha, than isolated interdigitating cells.Taken together, these results provide evidence that a subset of DCs enters primary follicles, armed with the capacity to attract and provide antigenic stimulation for T and B lymphocytes.

View Article: PubMed Central - PubMed

Affiliation: Serono Pharmaceutical Research Institute, CH-1228 Geneva, Switzerland.

ABSTRACT
Dendritic cells (DCs) are known to activate naive T cells to become effective helper cells. In addition, recent evidence suggests that DCs may influence naive B cells during the initial priming of antibody responses. In this study, using three-color confocal microscopy and three-dimensional immunohistograms, we have observed that in the first few days after a primary immunization, cells with dendritic morphology progressively localize within primary B cell follicles. These cells were identified by their ability to bind a fusion protein consisting of the terminal cysteine-rich portion of the mouse mannose receptor and the Fc portion of human immunoglobulin (Ig)G1 (CR-Fc). In situ, these CR-Fc binding cells express major histocompatibility complex class II, sialoadhesin, and CD11c and are negative for other markers identifying the myeloid DC lineage, such as (CD11b), macrophages (F4/80), follicular DCs (FDC-M2), B cells (B220), and T cells (CD4). Using CR-Fc binding capacity and flow cytometry, the cells were purified from the draining lymph nodes of mice 24 h after immunization. When injected into naive mice, these cells were able to prime T cells as well as induce production of antigen-specific IgM and IgG1. Furthermore, they produced significantly more of the lymphocyte chemoattractant, macrophage inflammatory protein (MIP)-1alpha, than isolated interdigitating cells. Taken together, these results provide evidence that a subset of DCs enters primary follicles, armed with the capacity to attract and provide antigenic stimulation for T and B lymphocytes.

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Chemokine production by the different subsets of DC. Day 6 BM-DCs, CR-Fc binding cells (CR-Fc+/N418+; isolated 24 h after OVA injection), and interdigitating cells (CR-Fc−/N418+; isolated 24 h after OVA injection) were isolated and cultured for 24 h in medium. Supernatants were then harvested, and the levels of MIP-1α and RANTES were determined by ELISA.
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Figure 8: Chemokine production by the different subsets of DC. Day 6 BM-DCs, CR-Fc binding cells (CR-Fc+/N418+; isolated 24 h after OVA injection), and interdigitating cells (CR-Fc−/N418+; isolated 24 h after OVA injection) were isolated and cultured for 24 h in medium. Supernatants were then harvested, and the levels of MIP-1α and RANTES were determined by ELISA.

Mentions: N418+/CR-Fc nonbinding cells (i.e., interdigitating cells) and N418+/CR-Fc binding cells isolated 24 h after antigen injection as well as N418+ DCs derived from bone marrow (BM-DCs) were assessed for their capacity to produce T cell chemoattractants. As shown in Fig. 8, RANTES (for regulated upon activation, normal T cell expressed and secreted) production followed the hierarchy of BM-DC < CR-Fc+/N418+ ≤ CR-Fc−/N418+ (interdigitating cell). In contrast, only N418+/CR-Fc binding cells produced MIP-1α.


A member of the dendritic cell family that enters B cell follicles and stimulates primary antibody responses identified by a mannose receptor fusion protein.

Berney C, Herren S, Power CA, Gordon S, Martinez-Pomares L, Kosco-Vilbois MH - J. Exp. Med. (1999)

Chemokine production by the different subsets of DC. Day 6 BM-DCs, CR-Fc binding cells (CR-Fc+/N418+; isolated 24 h after OVA injection), and interdigitating cells (CR-Fc−/N418+; isolated 24 h after OVA injection) were isolated and cultured for 24 h in medium. Supernatants were then harvested, and the levels of MIP-1α and RANTES were determined by ELISA.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2195630&req=5

Figure 8: Chemokine production by the different subsets of DC. Day 6 BM-DCs, CR-Fc binding cells (CR-Fc+/N418+; isolated 24 h after OVA injection), and interdigitating cells (CR-Fc−/N418+; isolated 24 h after OVA injection) were isolated and cultured for 24 h in medium. Supernatants were then harvested, and the levels of MIP-1α and RANTES were determined by ELISA.
Mentions: N418+/CR-Fc nonbinding cells (i.e., interdigitating cells) and N418+/CR-Fc binding cells isolated 24 h after antigen injection as well as N418+ DCs derived from bone marrow (BM-DCs) were assessed for their capacity to produce T cell chemoattractants. As shown in Fig. 8, RANTES (for regulated upon activation, normal T cell expressed and secreted) production followed the hierarchy of BM-DC < CR-Fc+/N418+ ≤ CR-Fc−/N418+ (interdigitating cell). In contrast, only N418+/CR-Fc binding cells produced MIP-1α.

Bottom Line: Dendritic cells (DCs) are known to activate naive T cells to become effective helper cells.Furthermore, they produced significantly more of the lymphocyte chemoattractant, macrophage inflammatory protein (MIP)-1alpha, than isolated interdigitating cells.Taken together, these results provide evidence that a subset of DCs enters primary follicles, armed with the capacity to attract and provide antigenic stimulation for T and B lymphocytes.

View Article: PubMed Central - PubMed

Affiliation: Serono Pharmaceutical Research Institute, CH-1228 Geneva, Switzerland.

ABSTRACT
Dendritic cells (DCs) are known to activate naive T cells to become effective helper cells. In addition, recent evidence suggests that DCs may influence naive B cells during the initial priming of antibody responses. In this study, using three-color confocal microscopy and three-dimensional immunohistograms, we have observed that in the first few days after a primary immunization, cells with dendritic morphology progressively localize within primary B cell follicles. These cells were identified by their ability to bind a fusion protein consisting of the terminal cysteine-rich portion of the mouse mannose receptor and the Fc portion of human immunoglobulin (Ig)G1 (CR-Fc). In situ, these CR-Fc binding cells express major histocompatibility complex class II, sialoadhesin, and CD11c and are negative for other markers identifying the myeloid DC lineage, such as (CD11b), macrophages (F4/80), follicular DCs (FDC-M2), B cells (B220), and T cells (CD4). Using CR-Fc binding capacity and flow cytometry, the cells were purified from the draining lymph nodes of mice 24 h after immunization. When injected into naive mice, these cells were able to prime T cells as well as induce production of antigen-specific IgM and IgG1. Furthermore, they produced significantly more of the lymphocyte chemoattractant, macrophage inflammatory protein (MIP)-1alpha, than isolated interdigitating cells. Taken together, these results provide evidence that a subset of DCs enters primary follicles, armed with the capacity to attract and provide antigenic stimulation for T and B lymphocytes.

Show MeSH