Limits...
Effective prophylaxis against rotavirus diarrhea using a combination of Lactobacillus rhamnosus GG and antibodies.

Pant N, Marcotte H, Brüssow H, Svensson L, Hammarström L - BMC Microbiol. (2007)

Bottom Line: The combination treatment reduced the diarrhea outcome measures significantly, prevented histopathological changes and reduced the virus load in the intestines.The advantages associated with immunoglobulins and probiotics based therapy is that the treatment provides a rapid therapeutic effect and is cost efficient.These components do not require special storage conditions and could potentially complement the rehydration therapy that is currently used.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden. neha.pant@ki.se

ABSTRACT

Background: Rotavirus is a worldwide cause of infectious infantile diarrhea that claims over 600,000 lives annually. Recently, two new vaccine candidates have been developed but their efficacy in developing countries, still remains to be proven. Oral delivery of specific immunoglobulins provides passive immunity and is a fast acting treatment for rotavirus diarrhea. Probiotic bacteria have also gained considerable attention lately as treatment for rotavirus diarrhea. Here we report an evaluation of the therapeutic potential of different probiotics and their combination with anti - rotavirus antibodies in a mouse model of rotavirus diarrhea.

Results: Of the six probiotic bacteria tested, Lactobacillus rhamnosus strain GG had the strongest influence in reducing prevalence, duration and severity of diarrhea and was therefore chosen for combination treatment with immunoglobulins. The combination treatment reduced the diarrhea outcome measures significantly, prevented histopathological changes and reduced the virus load in the intestines.

Conclusion: The advantages associated with immunoglobulins and probiotics based therapy is that the treatment provides a rapid therapeutic effect and is cost efficient. These components do not require special storage conditions and could potentially complement the rehydration therapy that is currently used.

Show MeSH

Related in: MedlinePlus

Real time PCR of intestinal tissue sections for RRV vp7 gene. Tissue samples were excised from small intestines and total cellular RNA was extracted. Real time PCR was performed for rotavirus vp7 gene. The bars represent geometric mean of the virus load after normalization with housekeeping gapdh gene. The combination of 10 μg HBC and L. rhamnosus GG was able to achieve a statistically significant reduction of virus load (as tested by Fischer's test). *** p < 0.0001, ** p = 0.0005, * p = 0.0016
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2194776&req=5

Figure 5: Real time PCR of intestinal tissue sections for RRV vp7 gene. Tissue samples were excised from small intestines and total cellular RNA was extracted. Real time PCR was performed for rotavirus vp7 gene. The bars represent geometric mean of the virus load after normalization with housekeeping gapdh gene. The combination of 10 μg HBC and L. rhamnosus GG was able to achieve a statistically significant reduction of virus load (as tested by Fischer's test). *** p < 0.0001, ** p = 0.0005, * p = 0.0016

Mentions: Since L. rhamnosus strain GG based therapy (either only bacteria or combined with HBC) significantly reduced diarrhea prevalence among the challenged mice and also reduced the associated histopathological changes, we wanted to confirm whether this therapy also had an effect on the virus load. Total cellular RNA isolated from intestinal tissue sections of pups treated with L. rhamnosus strain GG based treatment modalities (bacteria alone or combined with 10 μg HBC) were therefore analyzed by real time PCR for number of copies of the RRV vp7 gene. The virus load in infected, untreated mice was higher than in all the treatment groups (geometric mean 2038). As expected, no virus could be detected in uninfected negative control mice. Treatment with 100 μg of HBC was able to reduce the virus load in challenged mice well below the detection level of 10 vp7 copies and hence, corroborated the normal histology (geometric mean 2) (p < 0.0001). In comparison, the lower dose of 10 μg could not achieve a similar clearance of virus and the load was still high with a geometric mean of 102. Treatment with L. rhamnosus GG alone was also able to reduce the virus load significantly (geometric mean 53) (p = 0.001). The combined treatment of 10 μg of HBC and L. rhamnosus GG resulted in a statistically significant reduction of virus load in comparison to infected and untreated mice (geometric mean 15) (p = 0.0005) (Figure 5).


Effective prophylaxis against rotavirus diarrhea using a combination of Lactobacillus rhamnosus GG and antibodies.

Pant N, Marcotte H, Brüssow H, Svensson L, Hammarström L - BMC Microbiol. (2007)

Real time PCR of intestinal tissue sections for RRV vp7 gene. Tissue samples were excised from small intestines and total cellular RNA was extracted. Real time PCR was performed for rotavirus vp7 gene. The bars represent geometric mean of the virus load after normalization with housekeeping gapdh gene. The combination of 10 μg HBC and L. rhamnosus GG was able to achieve a statistically significant reduction of virus load (as tested by Fischer's test). *** p < 0.0001, ** p = 0.0005, * p = 0.0016
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2194776&req=5

Figure 5: Real time PCR of intestinal tissue sections for RRV vp7 gene. Tissue samples were excised from small intestines and total cellular RNA was extracted. Real time PCR was performed for rotavirus vp7 gene. The bars represent geometric mean of the virus load after normalization with housekeeping gapdh gene. The combination of 10 μg HBC and L. rhamnosus GG was able to achieve a statistically significant reduction of virus load (as tested by Fischer's test). *** p < 0.0001, ** p = 0.0005, * p = 0.0016
Mentions: Since L. rhamnosus strain GG based therapy (either only bacteria or combined with HBC) significantly reduced diarrhea prevalence among the challenged mice and also reduced the associated histopathological changes, we wanted to confirm whether this therapy also had an effect on the virus load. Total cellular RNA isolated from intestinal tissue sections of pups treated with L. rhamnosus strain GG based treatment modalities (bacteria alone or combined with 10 μg HBC) were therefore analyzed by real time PCR for number of copies of the RRV vp7 gene. The virus load in infected, untreated mice was higher than in all the treatment groups (geometric mean 2038). As expected, no virus could be detected in uninfected negative control mice. Treatment with 100 μg of HBC was able to reduce the virus load in challenged mice well below the detection level of 10 vp7 copies and hence, corroborated the normal histology (geometric mean 2) (p < 0.0001). In comparison, the lower dose of 10 μg could not achieve a similar clearance of virus and the load was still high with a geometric mean of 102. Treatment with L. rhamnosus GG alone was also able to reduce the virus load significantly (geometric mean 53) (p = 0.001). The combined treatment of 10 μg of HBC and L. rhamnosus GG resulted in a statistically significant reduction of virus load in comparison to infected and untreated mice (geometric mean 15) (p = 0.0005) (Figure 5).

Bottom Line: The combination treatment reduced the diarrhea outcome measures significantly, prevented histopathological changes and reduced the virus load in the intestines.The advantages associated with immunoglobulins and probiotics based therapy is that the treatment provides a rapid therapeutic effect and is cost efficient.These components do not require special storage conditions and could potentially complement the rehydration therapy that is currently used.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden. neha.pant@ki.se

ABSTRACT

Background: Rotavirus is a worldwide cause of infectious infantile diarrhea that claims over 600,000 lives annually. Recently, two new vaccine candidates have been developed but their efficacy in developing countries, still remains to be proven. Oral delivery of specific immunoglobulins provides passive immunity and is a fast acting treatment for rotavirus diarrhea. Probiotic bacteria have also gained considerable attention lately as treatment for rotavirus diarrhea. Here we report an evaluation of the therapeutic potential of different probiotics and their combination with anti - rotavirus antibodies in a mouse model of rotavirus diarrhea.

Results: Of the six probiotic bacteria tested, Lactobacillus rhamnosus strain GG had the strongest influence in reducing prevalence, duration and severity of diarrhea and was therefore chosen for combination treatment with immunoglobulins. The combination treatment reduced the diarrhea outcome measures significantly, prevented histopathological changes and reduced the virus load in the intestines.

Conclusion: The advantages associated with immunoglobulins and probiotics based therapy is that the treatment provides a rapid therapeutic effect and is cost efficient. These components do not require special storage conditions and could potentially complement the rehydration therapy that is currently used.

Show MeSH
Related in: MedlinePlus