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Effective prophylaxis against rotavirus diarrhea using a combination of Lactobacillus rhamnosus GG and antibodies.

Pant N, Marcotte H, Brüssow H, Svensson L, Hammarström L - BMC Microbiol. (2007)

Bottom Line: The combination treatment reduced the diarrhea outcome measures significantly, prevented histopathological changes and reduced the virus load in the intestines.The advantages associated with immunoglobulins and probiotics based therapy is that the treatment provides a rapid therapeutic effect and is cost efficient.These components do not require special storage conditions and could potentially complement the rehydration therapy that is currently used.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden. neha.pant@ki.se

ABSTRACT

Background: Rotavirus is a worldwide cause of infectious infantile diarrhea that claims over 600,000 lives annually. Recently, two new vaccine candidates have been developed but their efficacy in developing countries, still remains to be proven. Oral delivery of specific immunoglobulins provides passive immunity and is a fast acting treatment for rotavirus diarrhea. Probiotic bacteria have also gained considerable attention lately as treatment for rotavirus diarrhea. Here we report an evaluation of the therapeutic potential of different probiotics and their combination with anti - rotavirus antibodies in a mouse model of rotavirus diarrhea.

Results: Of the six probiotic bacteria tested, Lactobacillus rhamnosus strain GG had the strongest influence in reducing prevalence, duration and severity of diarrhea and was therefore chosen for combination treatment with immunoglobulins. The combination treatment reduced the diarrhea outcome measures significantly, prevented histopathological changes and reduced the virus load in the intestines.

Conclusion: The advantages associated with immunoglobulins and probiotics based therapy is that the treatment provides a rapid therapeutic effect and is cost efficient. These components do not require special storage conditions and could potentially complement the rehydration therapy that is currently used.

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Related in: MedlinePlus

Hematoxylin/Eosin stained sections of jejunum from mice treated with different formulations. Tissue sections were excised and embedded in paraffin. HE staining was performed by standard protocols and the samples assessed blindly for signs of rotavirus infection. (A) infected and untreated mice shows typical signs of grave rotavirus infection with swollen and vacuolized villus tips. (B) L. rhamnosus GG treated mice show moderately resolved histopathology similar to (C) 10 μg/dose HBC treated mice. (D) L. rhamnosus GG combined with HBC is able to resolve the histopathology to normalcy. (E) no histopathology associated with treatment with 100 μg/dose HBC. (F) uninfected control mice.
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Figure 4: Hematoxylin/Eosin stained sections of jejunum from mice treated with different formulations. Tissue sections were excised and embedded in paraffin. HE staining was performed by standard protocols and the samples assessed blindly for signs of rotavirus infection. (A) infected and untreated mice shows typical signs of grave rotavirus infection with swollen and vacuolized villus tips. (B) L. rhamnosus GG treated mice show moderately resolved histopathology similar to (C) 10 μg/dose HBC treated mice. (D) L. rhamnosus GG combined with HBC is able to resolve the histopathology to normalcy. (E) no histopathology associated with treatment with 100 μg/dose HBC. (F) uninfected control mice.

Mentions: Formalin fixed intestinal tissue sections from mice treated with different treatment modalities were blindly analyzed for histopathological changes associated with rotavirus infection [17]. The RRV infected, untreated group presented a typical histology associated with rotavirus infection with swollen villus tips and vacuolization. The villus tips were unstainable due to epithelial cell death (Figure 4A). The histo-pathology showed reduced vacuolization in pups receiving L. rhamnosus GG or 10 μg HBC alone (Figure 4B and 4C). In comparison, the combined treatment of L. rhamnosus GG with 10 μg of HBC prevented histological changes completely (Figure 4D) as was also seen with 100 μg of HBC (Figure 4E). The negative control mice that were not infected showed a normal histology (Figure 4F).


Effective prophylaxis against rotavirus diarrhea using a combination of Lactobacillus rhamnosus GG and antibodies.

Pant N, Marcotte H, Brüssow H, Svensson L, Hammarström L - BMC Microbiol. (2007)

Hematoxylin/Eosin stained sections of jejunum from mice treated with different formulations. Tissue sections were excised and embedded in paraffin. HE staining was performed by standard protocols and the samples assessed blindly for signs of rotavirus infection. (A) infected and untreated mice shows typical signs of grave rotavirus infection with swollen and vacuolized villus tips. (B) L. rhamnosus GG treated mice show moderately resolved histopathology similar to (C) 10 μg/dose HBC treated mice. (D) L. rhamnosus GG combined with HBC is able to resolve the histopathology to normalcy. (E) no histopathology associated with treatment with 100 μg/dose HBC. (F) uninfected control mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2194776&req=5

Figure 4: Hematoxylin/Eosin stained sections of jejunum from mice treated with different formulations. Tissue sections were excised and embedded in paraffin. HE staining was performed by standard protocols and the samples assessed blindly for signs of rotavirus infection. (A) infected and untreated mice shows typical signs of grave rotavirus infection with swollen and vacuolized villus tips. (B) L. rhamnosus GG treated mice show moderately resolved histopathology similar to (C) 10 μg/dose HBC treated mice. (D) L. rhamnosus GG combined with HBC is able to resolve the histopathology to normalcy. (E) no histopathology associated with treatment with 100 μg/dose HBC. (F) uninfected control mice.
Mentions: Formalin fixed intestinal tissue sections from mice treated with different treatment modalities were blindly analyzed for histopathological changes associated with rotavirus infection [17]. The RRV infected, untreated group presented a typical histology associated with rotavirus infection with swollen villus tips and vacuolization. The villus tips were unstainable due to epithelial cell death (Figure 4A). The histo-pathology showed reduced vacuolization in pups receiving L. rhamnosus GG or 10 μg HBC alone (Figure 4B and 4C). In comparison, the combined treatment of L. rhamnosus GG with 10 μg of HBC prevented histological changes completely (Figure 4D) as was also seen with 100 μg of HBC (Figure 4E). The negative control mice that were not infected showed a normal histology (Figure 4F).

Bottom Line: The combination treatment reduced the diarrhea outcome measures significantly, prevented histopathological changes and reduced the virus load in the intestines.The advantages associated with immunoglobulins and probiotics based therapy is that the treatment provides a rapid therapeutic effect and is cost efficient.These components do not require special storage conditions and could potentially complement the rehydration therapy that is currently used.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Institutet at Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden. neha.pant@ki.se

ABSTRACT

Background: Rotavirus is a worldwide cause of infectious infantile diarrhea that claims over 600,000 lives annually. Recently, two new vaccine candidates have been developed but their efficacy in developing countries, still remains to be proven. Oral delivery of specific immunoglobulins provides passive immunity and is a fast acting treatment for rotavirus diarrhea. Probiotic bacteria have also gained considerable attention lately as treatment for rotavirus diarrhea. Here we report an evaluation of the therapeutic potential of different probiotics and their combination with anti - rotavirus antibodies in a mouse model of rotavirus diarrhea.

Results: Of the six probiotic bacteria tested, Lactobacillus rhamnosus strain GG had the strongest influence in reducing prevalence, duration and severity of diarrhea and was therefore chosen for combination treatment with immunoglobulins. The combination treatment reduced the diarrhea outcome measures significantly, prevented histopathological changes and reduced the virus load in the intestines.

Conclusion: The advantages associated with immunoglobulins and probiotics based therapy is that the treatment provides a rapid therapeutic effect and is cost efficient. These components do not require special storage conditions and could potentially complement the rehydration therapy that is currently used.

Show MeSH
Related in: MedlinePlus