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Isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer.

Thiry J, Widén F, Grégoire F, Linden A, Belák S, Thiry E - BMC Vet. Res. (2007)

Bottom Line: Besides the close relationship between all ruminant alphaherpesviruses, the phylogenetic analysis revealed that the isolate clustered with CvHV-1.Data demonstrate that a CvHV-1 strain, named Anlier, circulates in wild red deer in continental Europe.All together, these results improve our understanding of ruminant alphaherpesviruses.

View Article: PubMed Central - HTML - PubMed

Affiliation: Virology and Viral Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium. julien.thiry@ulg.ac.be

ABSTRACT

Background: The genus Varicellovirus of the Herpesviridae subfamily Alphaherpesvirinae includes a cluster of viruses antigenically and genetically related to bovine herpesvirus 1 (BoHV-1): namely bovine herpesvirus 5 (BoHV-5), bubaline herpesvirus 1 (BuHV-1), caprine herpesvirus 1 (CpHV-1), cervid herpesviruses 1 (CvHV-1) and 2 (CvHV-2) and elk herpesvirus 1 (ElkHV-1). Considering the serological relationship between these ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV-1 related virus infection in wild and domestic ruminant species. In this way, a recent investigation has indicated, in Belgium, a high increase in the serological prevalence of BoHV-1 related virus infection in free-ranging red deer population. In this context, it has been decided to investigate the presence of an alphaherpesvirus spreading in the Belgian free-ranging red deer population.

Results: The current study reports the first isolation in a free-ranging red deer of a BoHV-1 closely related virus. The isolate was antigenically, genomically and genetically characterised by comparison with several ruminant alphaherpesvirus. Immunofluorescence assays revealed the isolate was antigenically distinct from bovine and caprine alphaherpesviruses. Similarly, BamHI and BstEII restriction analyses demonstrated the genomic difference between the isolate and the other ruminant alphaherpesviruses. Next, the sequencing of selected parts of UL27 and US8 genes showed a high degree of homologies between each BoHV-1 related ruminant alphaherpesvirus and the isolate. Besides the close relationship between all ruminant alphaherpesviruses, the phylogenetic analysis revealed that the isolate clustered with CvHV-1.

Conclusion: The first isolation of a virus closely related to BoHV-1 in a free-ranging red deer is reported. Data demonstrate that a CvHV-1 strain, named Anlier, circulates in wild red deer in continental Europe. Anlier strain show consistent differences with the virus isolated from Scottish farmed red deer. All together, these results improve our understanding of ruminant alphaherpesviruses.

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Alignment of a selected part of US8 sequence of the Anlier isolate and ruminant alphaherpesviruses.
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Figure 4: Alignment of a selected part of US8 sequence of the Anlier isolate and ruminant alphaherpesviruses.

Mentions: To further characterise the Anlier isolate, different regions of the genome were sequenced (Figure 3). Partial sequence data from UL27 encoding the glycoprotein B (gB) and US8 encoding the glycoprotein E (gE) were obtained for eight ruminant alphaherpesviruses including the two BoHV-1 subtypes and the Anlier isolate. These sequences represented two different degrees of conservation among ruminant alphaherpesviruses genes. The sequence lengths were 444 nucleotides for UL27 and varied between 597 and 609 nucleotides for US8. By analysing multiple nucleotide sequences alignments in the US8 sequence, CvHV-1 and CvHV-2 differed from each other by 96 out of 609 bases, CvHV-1 and ElkHV-1 by 15 out of 609 bases, ElkHV-1 and the Anlier isolate by 22 out of 609 bases, CvHV-2 and the Anlier isolate by 99 out of 609 bases, and CvHV-1 and the Anlier isolate by 16 out of 609 bases (Figure 4). Tables 1 and 2 compare the percentage of nucleotide and amino acid sequence identity between each ruminant alphaherpesviruses. Phylogenetic analyses were also performed to assess the relationship between the Anlier isolate and other ruminant alphaherpesviruses (Figure 5). BoHV-5 and BuHV-1 clustered together and are the most closely related to BoHV-1 1.1 and BoHV-1 1.2. CpHV-1 is the most diverging ruminant alphaherpesvirus. CvHV-1 is more related to BoHV-1 than ElkHV-1 and CvHV-2 which is the most closely related to CpHV-1. The Anlier isolate clustered with CvHV-1 and the most closely related virus is ElkHV-1. The same topography was obtained for phylogenetic trees based on UL27 (Figure 5a) and US8 nucleotide sequences (Figure 5b). Taken together, these data revealed high degrees of homology between each ruminant alphaherpesvirus, the highest being observed between CvHV-1 and the Anlier isolate demonstrating the identification of a new CvHV-1 strain.


Isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer.

Thiry J, Widén F, Grégoire F, Linden A, Belák S, Thiry E - BMC Vet. Res. (2007)

Alignment of a selected part of US8 sequence of the Anlier isolate and ruminant alphaherpesviruses.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2194762&req=5

Figure 4: Alignment of a selected part of US8 sequence of the Anlier isolate and ruminant alphaherpesviruses.
Mentions: To further characterise the Anlier isolate, different regions of the genome were sequenced (Figure 3). Partial sequence data from UL27 encoding the glycoprotein B (gB) and US8 encoding the glycoprotein E (gE) were obtained for eight ruminant alphaherpesviruses including the two BoHV-1 subtypes and the Anlier isolate. These sequences represented two different degrees of conservation among ruminant alphaherpesviruses genes. The sequence lengths were 444 nucleotides for UL27 and varied between 597 and 609 nucleotides for US8. By analysing multiple nucleotide sequences alignments in the US8 sequence, CvHV-1 and CvHV-2 differed from each other by 96 out of 609 bases, CvHV-1 and ElkHV-1 by 15 out of 609 bases, ElkHV-1 and the Anlier isolate by 22 out of 609 bases, CvHV-2 and the Anlier isolate by 99 out of 609 bases, and CvHV-1 and the Anlier isolate by 16 out of 609 bases (Figure 4). Tables 1 and 2 compare the percentage of nucleotide and amino acid sequence identity between each ruminant alphaherpesviruses. Phylogenetic analyses were also performed to assess the relationship between the Anlier isolate and other ruminant alphaherpesviruses (Figure 5). BoHV-5 and BuHV-1 clustered together and are the most closely related to BoHV-1 1.1 and BoHV-1 1.2. CpHV-1 is the most diverging ruminant alphaherpesvirus. CvHV-1 is more related to BoHV-1 than ElkHV-1 and CvHV-2 which is the most closely related to CpHV-1. The Anlier isolate clustered with CvHV-1 and the most closely related virus is ElkHV-1. The same topography was obtained for phylogenetic trees based on UL27 (Figure 5a) and US8 nucleotide sequences (Figure 5b). Taken together, these data revealed high degrees of homology between each ruminant alphaherpesvirus, the highest being observed between CvHV-1 and the Anlier isolate demonstrating the identification of a new CvHV-1 strain.

Bottom Line: Besides the close relationship between all ruminant alphaherpesviruses, the phylogenetic analysis revealed that the isolate clustered with CvHV-1.Data demonstrate that a CvHV-1 strain, named Anlier, circulates in wild red deer in continental Europe.All together, these results improve our understanding of ruminant alphaherpesviruses.

View Article: PubMed Central - HTML - PubMed

Affiliation: Virology and Viral Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium. julien.thiry@ulg.ac.be

ABSTRACT

Background: The genus Varicellovirus of the Herpesviridae subfamily Alphaherpesvirinae includes a cluster of viruses antigenically and genetically related to bovine herpesvirus 1 (BoHV-1): namely bovine herpesvirus 5 (BoHV-5), bubaline herpesvirus 1 (BuHV-1), caprine herpesvirus 1 (CpHV-1), cervid herpesviruses 1 (CvHV-1) and 2 (CvHV-2) and elk herpesvirus 1 (ElkHV-1). Considering the serological relationship between these ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV-1 related virus infection in wild and domestic ruminant species. In this way, a recent investigation has indicated, in Belgium, a high increase in the serological prevalence of BoHV-1 related virus infection in free-ranging red deer population. In this context, it has been decided to investigate the presence of an alphaherpesvirus spreading in the Belgian free-ranging red deer population.

Results: The current study reports the first isolation in a free-ranging red deer of a BoHV-1 closely related virus. The isolate was antigenically, genomically and genetically characterised by comparison with several ruminant alphaherpesvirus. Immunofluorescence assays revealed the isolate was antigenically distinct from bovine and caprine alphaherpesviruses. Similarly, BamHI and BstEII restriction analyses demonstrated the genomic difference between the isolate and the other ruminant alphaherpesviruses. Next, the sequencing of selected parts of UL27 and US8 genes showed a high degree of homologies between each BoHV-1 related ruminant alphaherpesvirus and the isolate. Besides the close relationship between all ruminant alphaherpesviruses, the phylogenetic analysis revealed that the isolate clustered with CvHV-1.

Conclusion: The first isolation of a virus closely related to BoHV-1 in a free-ranging red deer is reported. Data demonstrate that a CvHV-1 strain, named Anlier, circulates in wild red deer in continental Europe. Anlier strain show consistent differences with the virus isolated from Scottish farmed red deer. All together, these results improve our understanding of ruminant alphaherpesviruses.

Show MeSH
Related in: MedlinePlus