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Differential expression of viral PAMP receptors mRNA in peripheral blood of patients with chronic hepatitis C infection.

Atencia R, Bustamante FJ, Valdivieso A, Arrieta A, Riñón M, Prada A, Maruri N - BMC Infect. Dis. (2007)

Bottom Line: IFN-alpha, TLR3, TLR7 and RIG-I levels in peripheral blood from healthy controls (n = 18) and chronic HCV patients (n = 18) were quantified by real-time polymerase chain reaction.Our results show that IFN-alpha, TLR3, TLR7 and RIG-I mRNA levels are significantly down-regulated in patients with chronic HCV infection when compared with healthy controls.We also found that the measured levels of TLR3 and TLR7, but not RIG-I, correlated significantly with those of IFN-alpha Monitoring the expression of RNA-sensing receptors like TLR3, TLR7 and RIG-I during the different clinical stages of infection could bring a new source of data about the prognosis of disease.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratorio de Inmunología, Hospital de Cruces, Barakaldo, Vizcaya, Spain. ratencia@eitd.net

ABSTRACT

Background: Pathogen-associated molecular patterns (PAMP) receptors play a key role in the early host response to viruses. In this work, we determined mRNA levels of two members of the Toll-like Receptors family, (TLR3 and TLR7) and the helicase RIG-I, all of three recognizing viral RNA products, in peripheral blood of healthy donors and hepatitis C virus (HCV) patients, to observe if their transcripts are altered in this disease.

Methods: IFN-alpha, TLR3, TLR7 and RIG-I levels in peripheral blood from healthy controls (n = 18) and chronic HCV patients (n = 18) were quantified by real-time polymerase chain reaction.

Results: Our results show that IFN-alpha, TLR3, TLR7 and RIG-I mRNA levels are significantly down-regulated in patients with chronic HCV infection when compared with healthy controls. We also found that the measured levels of TLR3 and TLR7, but not RIG-I, correlated significantly with those of IFN-alpha

Conclusion: Monitoring the expression of RNA-sensing receptors like TLR3, TLR7 and RIG-I during the different clinical stages of infection could bring a new source of data about the prognosis of disease.

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Related in: MedlinePlus

Correlation plots between the mRNA relative levels of IFN-α and PAMP receptors. Each plot shows the correlation between the expression levels of IFN-α against those of TLR3 (A), TLR7 (B) or RIG-I (C)
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Figure 2: Correlation plots between the mRNA relative levels of IFN-α and PAMP receptors. Each plot shows the correlation between the expression levels of IFN-α against those of TLR3 (A), TLR7 (B) or RIG-I (C)

Mentions: The statistical regression analysis of the quantitative RT-PCR data and clinical data showed no correlation between the expression levels of the studied receptors and other factors as viral load, AST/ALT levels or cirrhosis. However, we found that the measured levels of TLR3 and TLR7, but not RIG-I, correlated significantly with those of IFN-α(r = 0.670, p = 0.004 and r = 0.657, p = 0.005 respectively. Figure 2).


Differential expression of viral PAMP receptors mRNA in peripheral blood of patients with chronic hepatitis C infection.

Atencia R, Bustamante FJ, Valdivieso A, Arrieta A, Riñón M, Prada A, Maruri N - BMC Infect. Dis. (2007)

Correlation plots between the mRNA relative levels of IFN-α and PAMP receptors. Each plot shows the correlation between the expression levels of IFN-α against those of TLR3 (A), TLR7 (B) or RIG-I (C)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2194715&req=5

Figure 2: Correlation plots between the mRNA relative levels of IFN-α and PAMP receptors. Each plot shows the correlation between the expression levels of IFN-α against those of TLR3 (A), TLR7 (B) or RIG-I (C)
Mentions: The statistical regression analysis of the quantitative RT-PCR data and clinical data showed no correlation between the expression levels of the studied receptors and other factors as viral load, AST/ALT levels or cirrhosis. However, we found that the measured levels of TLR3 and TLR7, but not RIG-I, correlated significantly with those of IFN-α(r = 0.670, p = 0.004 and r = 0.657, p = 0.005 respectively. Figure 2).

Bottom Line: IFN-alpha, TLR3, TLR7 and RIG-I levels in peripheral blood from healthy controls (n = 18) and chronic HCV patients (n = 18) were quantified by real-time polymerase chain reaction.Our results show that IFN-alpha, TLR3, TLR7 and RIG-I mRNA levels are significantly down-regulated in patients with chronic HCV infection when compared with healthy controls.We also found that the measured levels of TLR3 and TLR7, but not RIG-I, correlated significantly with those of IFN-alpha Monitoring the expression of RNA-sensing receptors like TLR3, TLR7 and RIG-I during the different clinical stages of infection could bring a new source of data about the prognosis of disease.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratorio de Inmunología, Hospital de Cruces, Barakaldo, Vizcaya, Spain. ratencia@eitd.net

ABSTRACT

Background: Pathogen-associated molecular patterns (PAMP) receptors play a key role in the early host response to viruses. In this work, we determined mRNA levels of two members of the Toll-like Receptors family, (TLR3 and TLR7) and the helicase RIG-I, all of three recognizing viral RNA products, in peripheral blood of healthy donors and hepatitis C virus (HCV) patients, to observe if their transcripts are altered in this disease.

Methods: IFN-alpha, TLR3, TLR7 and RIG-I levels in peripheral blood from healthy controls (n = 18) and chronic HCV patients (n = 18) were quantified by real-time polymerase chain reaction.

Results: Our results show that IFN-alpha, TLR3, TLR7 and RIG-I mRNA levels are significantly down-regulated in patients with chronic HCV infection when compared with healthy controls. We also found that the measured levels of TLR3 and TLR7, but not RIG-I, correlated significantly with those of IFN-alpha

Conclusion: Monitoring the expression of RNA-sensing receptors like TLR3, TLR7 and RIG-I during the different clinical stages of infection could bring a new source of data about the prognosis of disease.

Show MeSH
Related in: MedlinePlus