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Neonatal idiotypic exposure alters subsequent cytokine, pathology, and survival patterns in experimental Schistosoma mansoni infections.

Montesano MA, Colley DG, Eloi-Santos S, Freeman GL, Secor WE - J. Exp. Med. (1999)

Bottom Line: We have used an experimental murine system to address the role of Ids in this immunoregulation.Sera from mice with 8-wk Schistosoma mansoni infection, chronic (20-wk infection) moderate splenomegaly syndrome (MSS), or chronic hypersplenomegaly syndrome (HSS) were passed over an S. mansoni soluble egg antigen (SEA) immunoaffinity column to prepare Ids (8WkId, MSS Id, HSS Id).Furthermore, neonatal exposure to 8WkId or MSS Id, but not NoMoIgG or HSS Id, led to significantly smaller granuloma size and lower hepatic fibrosis levels in infected mice.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiologia, Immunologia e Parasitologia, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil 36036.

ABSTRACT
Exposure to maternal idiotypes (Ids) or antigens might predispose a child to develop an immunoregulated, asymptomatic clinical presentation of schistosomiasis. We have used an experimental murine system to address the role of Ids in this immunoregulation. Sera from mice with 8-wk Schistosoma mansoni infection, chronic (20-wk infection) moderate splenomegaly syndrome (MSS), or chronic hypersplenomegaly syndrome (HSS) were passed over an S. mansoni soluble egg antigen (SEA) immunoaffinity column to prepare Ids (8WkId, MSS Id, HSS Id). Newborn mice were injected with 8WkId, MSS Id, HSS Id, or normal mouse immunoglobulin (NoMoIgG) and infected with S. mansoni 8 wk later. Mice exposed to 8WkId or MSS Id as newborns had prolonged survival and decreased morbidity compared with mice that received HSS Id or NoMoIgG. When stimulated with SEA, 8WkId, or MSS Id, spleen cells from mice neonatally injected with 8WkId or MSS Id produced more interferon gamma than spleen cells from mice neonatally injected with HSS Id or NoMoIgG. Furthermore, neonatal exposure to 8WkId or MSS Id, but not NoMoIgG or HSS Id, led to significantly smaller granuloma size and lower hepatic fibrosis levels in infected mice. Together, these results indicate that perinatal exposure to appropriate anti-SEA Ids induces long-term effects on survival, pathology, and immune response patterns in mice subsequently infected with S. mansoni.

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Related in: MedlinePlus

Mice neonatally injected with CRI (8WkId or MSS Id) produce elevated levels of SEA- and SWAP-specific IgG2a or IgG2b at 8 wk  of infection. The sera of the mice neonatally injected with Id, infected at  8 wk of age, and killed at 8 wk after infection were screened for specific  isotype responses to SEA (A) and SWAP (B). Mice that received 8WkId  or MSS Id as neonates had significantly higher levels of antigen-specific  IgG2a and IgG2b than mice that were injected with NoMoIgG or HSS  Id at birth (*P < 0.001).
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Figure 6: Mice neonatally injected with CRI (8WkId or MSS Id) produce elevated levels of SEA- and SWAP-specific IgG2a or IgG2b at 8 wk of infection. The sera of the mice neonatally injected with Id, infected at 8 wk of age, and killed at 8 wk after infection were screened for specific isotype responses to SEA (A) and SWAP (B). Mice that received 8WkId or MSS Id as neonates had significantly higher levels of antigen-specific IgG2a and IgG2b than mice that were injected with NoMoIgG or HSS Id at birth (*P < 0.001).

Mentions: Because isotype switching to IgG2a in mice is at least in part effected by IFN-γ (15, 16), we investigated the anti-SEA IgG subclasses present in sera of the neonatally manipulated mice (Fig. 6). At 8 wk of infection, SEA- and SWAP-specific IgG2a and IgG2b levels in sera of mice neonatally injected with 8WkId or MSS Id were higher than those of mice neonatally injected with NoMoIgG or HSS Id (P < 0.0001). SEA- and SWAP-specific IgG1 levels were similar among the various neonatal injection groups, indicating that the differences in granuloma and fibrotic regulation were not related to a failure to make schistosome-specific Abs in mice neonatally treated with NoMoIgG or HSS Id.


Neonatal idiotypic exposure alters subsequent cytokine, pathology, and survival patterns in experimental Schistosoma mansoni infections.

Montesano MA, Colley DG, Eloi-Santos S, Freeman GL, Secor WE - J. Exp. Med. (1999)

Mice neonatally injected with CRI (8WkId or MSS Id) produce elevated levels of SEA- and SWAP-specific IgG2a or IgG2b at 8 wk  of infection. The sera of the mice neonatally injected with Id, infected at  8 wk of age, and killed at 8 wk after infection were screened for specific  isotype responses to SEA (A) and SWAP (B). Mice that received 8WkId  or MSS Id as neonates had significantly higher levels of antigen-specific  IgG2a and IgG2b than mice that were injected with NoMoIgG or HSS  Id at birth (*P < 0.001).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2192931&req=5

Figure 6: Mice neonatally injected with CRI (8WkId or MSS Id) produce elevated levels of SEA- and SWAP-specific IgG2a or IgG2b at 8 wk of infection. The sera of the mice neonatally injected with Id, infected at 8 wk of age, and killed at 8 wk after infection were screened for specific isotype responses to SEA (A) and SWAP (B). Mice that received 8WkId or MSS Id as neonates had significantly higher levels of antigen-specific IgG2a and IgG2b than mice that were injected with NoMoIgG or HSS Id at birth (*P < 0.001).
Mentions: Because isotype switching to IgG2a in mice is at least in part effected by IFN-γ (15, 16), we investigated the anti-SEA IgG subclasses present in sera of the neonatally manipulated mice (Fig. 6). At 8 wk of infection, SEA- and SWAP-specific IgG2a and IgG2b levels in sera of mice neonatally injected with 8WkId or MSS Id were higher than those of mice neonatally injected with NoMoIgG or HSS Id (P < 0.0001). SEA- and SWAP-specific IgG1 levels were similar among the various neonatal injection groups, indicating that the differences in granuloma and fibrotic regulation were not related to a failure to make schistosome-specific Abs in mice neonatally treated with NoMoIgG or HSS Id.

Bottom Line: We have used an experimental murine system to address the role of Ids in this immunoregulation.Sera from mice with 8-wk Schistosoma mansoni infection, chronic (20-wk infection) moderate splenomegaly syndrome (MSS), or chronic hypersplenomegaly syndrome (HSS) were passed over an S. mansoni soluble egg antigen (SEA) immunoaffinity column to prepare Ids (8WkId, MSS Id, HSS Id).Furthermore, neonatal exposure to 8WkId or MSS Id, but not NoMoIgG or HSS Id, led to significantly smaller granuloma size and lower hepatic fibrosis levels in infected mice.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiologia, Immunologia e Parasitologia, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil 36036.

ABSTRACT
Exposure to maternal idiotypes (Ids) or antigens might predispose a child to develop an immunoregulated, asymptomatic clinical presentation of schistosomiasis. We have used an experimental murine system to address the role of Ids in this immunoregulation. Sera from mice with 8-wk Schistosoma mansoni infection, chronic (20-wk infection) moderate splenomegaly syndrome (MSS), or chronic hypersplenomegaly syndrome (HSS) were passed over an S. mansoni soluble egg antigen (SEA) immunoaffinity column to prepare Ids (8WkId, MSS Id, HSS Id). Newborn mice were injected with 8WkId, MSS Id, HSS Id, or normal mouse immunoglobulin (NoMoIgG) and infected with S. mansoni 8 wk later. Mice exposed to 8WkId or MSS Id as newborns had prolonged survival and decreased morbidity compared with mice that received HSS Id or NoMoIgG. When stimulated with SEA, 8WkId, or MSS Id, spleen cells from mice neonatally injected with 8WkId or MSS Id produced more interferon gamma than spleen cells from mice neonatally injected with HSS Id or NoMoIgG. Furthermore, neonatal exposure to 8WkId or MSS Id, but not NoMoIgG or HSS Id, led to significantly smaller granuloma size and lower hepatic fibrosis levels in infected mice. Together, these results indicate that perinatal exposure to appropriate anti-SEA Ids induces long-term effects on survival, pathology, and immune response patterns in mice subsequently infected with S. mansoni.

Show MeSH
Related in: MedlinePlus