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Tetraether membrane lipids of Candidatus "Aciduliprofundum boonei", a cultivated obligate thermoacidophilic euryarchaeote from deep-sea hydrothermal vents.

Schouten S, Baas M, Hopmans EC, Reysenbach AL, Damsté JS - Extremophiles (2007)

Bottom Line: The predominant core membrane lipids in this thermophilic euryarchaeote were found to be composed of glycerol dibiphytanyl glycerol tetraethers (GDGTs) containing 0-4 cyclopentyl moieties.In addition, GDGTs with an additional covalent bond between the isoprenoid hydrocarbon chains, so-called H-shaped GDGTs, were present.The latter core lipids have been rarely reported previously.

View Article: PubMed Central - PubMed

Affiliation: Department of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands. schouten@nioz.nl

ABSTRACT
The lipid composition of Candidatus "Aciduliprofundum boonei", the only cultivated representative of archaea falling in the DHVE2 phylogenetic cluster, a group of microorganisms ubiquitously occurring at hydrothermal vents, was studied. The predominant core membrane lipids in this thermophilic euryarchaeote were found to be composed of glycerol dibiphytanyl glycerol tetraethers (GDGTs) containing 0-4 cyclopentyl moieties. In addition, GDGTs with an additional covalent bond between the isoprenoid hydrocarbon chains, so-called H-shaped GDGTs, were present. The latter core lipids have been rarely reported previously. Intact polar lipid analysis revealed that they predominantly consist of GDGTs with a phospho-glycerol headgroup.

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HPLC/ESI/MS base peak chromatogram of intact polar lipids in Bligh Dyer extract of cell material of Candidatus “Aciduliprofundum boonei”. Inset shows the electrospray ionization mass spectrum of the major peak in the chromatogram with tentative structural identification
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Fig2: HPLC/ESI/MS base peak chromatogram of intact polar lipids in Bligh Dyer extract of cell material of Candidatus “Aciduliprofundum boonei”. Inset shows the electrospray ionization mass spectrum of the major peak in the chromatogram with tentative structural identification

Mentions: HPLC/ESI-MS analysis of the Bligh–Dyer extract of cell material of A. boonei revealed one major peak and one minor peak (Fig. 2). The mass spectrum of the major peak is dominated by an ion at m/z 1,456 with minor ions 15 and 22 Da higher (insert Fig. 2). These latter ions are typical product of ammonia and sodium adductions, respectively, to protonated molecules in the ESI interface and suggests that the ion at m/z 1,456 represents the [M + H]+ ion (cf. Sturt et al. 2004). As the major core lipid is GDGT II with a [M + H]+ ion of 1,302, it suggests that the head group has a mass of 154 Da in agreement with a phospho-glycerol (PG) moiety (Sturt et al. 2004). The mass spectrum of the minor peak eluting slightly later is similar to that of the major peak except that all ions are shifted 2 Da lower. In view of the results obtained with the core lipids it suggests that this peak represents H-shaped GDGTs with a PG head group. PG moieties are not uncommon as head groups of GDGTs in hyperthermophilic Archaea, though they are usually accompanied by a hexose head group attached to the other glycerol moiety (Koga and Morii 2005 and references cited therein). However, these compounds were not detected in A. boonei although we cannot exclude that they might be present in minor amounts.Fig. 2


Tetraether membrane lipids of Candidatus "Aciduliprofundum boonei", a cultivated obligate thermoacidophilic euryarchaeote from deep-sea hydrothermal vents.

Schouten S, Baas M, Hopmans EC, Reysenbach AL, Damsté JS - Extremophiles (2007)

HPLC/ESI/MS base peak chromatogram of intact polar lipids in Bligh Dyer extract of cell material of Candidatus “Aciduliprofundum boonei”. Inset shows the electrospray ionization mass spectrum of the major peak in the chromatogram with tentative structural identification
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2175526&req=5

Fig2: HPLC/ESI/MS base peak chromatogram of intact polar lipids in Bligh Dyer extract of cell material of Candidatus “Aciduliprofundum boonei”. Inset shows the electrospray ionization mass spectrum of the major peak in the chromatogram with tentative structural identification
Mentions: HPLC/ESI-MS analysis of the Bligh–Dyer extract of cell material of A. boonei revealed one major peak and one minor peak (Fig. 2). The mass spectrum of the major peak is dominated by an ion at m/z 1,456 with minor ions 15 and 22 Da higher (insert Fig. 2). These latter ions are typical product of ammonia and sodium adductions, respectively, to protonated molecules in the ESI interface and suggests that the ion at m/z 1,456 represents the [M + H]+ ion (cf. Sturt et al. 2004). As the major core lipid is GDGT II with a [M + H]+ ion of 1,302, it suggests that the head group has a mass of 154 Da in agreement with a phospho-glycerol (PG) moiety (Sturt et al. 2004). The mass spectrum of the minor peak eluting slightly later is similar to that of the major peak except that all ions are shifted 2 Da lower. In view of the results obtained with the core lipids it suggests that this peak represents H-shaped GDGTs with a PG head group. PG moieties are not uncommon as head groups of GDGTs in hyperthermophilic Archaea, though they are usually accompanied by a hexose head group attached to the other glycerol moiety (Koga and Morii 2005 and references cited therein). However, these compounds were not detected in A. boonei although we cannot exclude that they might be present in minor amounts.Fig. 2

Bottom Line: The predominant core membrane lipids in this thermophilic euryarchaeote were found to be composed of glycerol dibiphytanyl glycerol tetraethers (GDGTs) containing 0-4 cyclopentyl moieties.In addition, GDGTs with an additional covalent bond between the isoprenoid hydrocarbon chains, so-called H-shaped GDGTs, were present.The latter core lipids have been rarely reported previously.

View Article: PubMed Central - PubMed

Affiliation: Department of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands. schouten@nioz.nl

ABSTRACT
The lipid composition of Candidatus "Aciduliprofundum boonei", the only cultivated representative of archaea falling in the DHVE2 phylogenetic cluster, a group of microorganisms ubiquitously occurring at hydrothermal vents, was studied. The predominant core membrane lipids in this thermophilic euryarchaeote were found to be composed of glycerol dibiphytanyl glycerol tetraethers (GDGTs) containing 0-4 cyclopentyl moieties. In addition, GDGTs with an additional covalent bond between the isoprenoid hydrocarbon chains, so-called H-shaped GDGTs, were present. The latter core lipids have been rarely reported previously. Intact polar lipid analysis revealed that they predominantly consist of GDGTs with a phospho-glycerol headgroup.

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