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Tetraether membrane lipids of Candidatus "Aciduliprofundum boonei", a cultivated obligate thermoacidophilic euryarchaeote from deep-sea hydrothermal vents.

Schouten S, Baas M, Hopmans EC, Reysenbach AL, Damsté JS - Extremophiles (2007)

Bottom Line: The predominant core membrane lipids in this thermophilic euryarchaeote were found to be composed of glycerol dibiphytanyl glycerol tetraethers (GDGTs) containing 0-4 cyclopentyl moieties.In addition, GDGTs with an additional covalent bond between the isoprenoid hydrocarbon chains, so-called H-shaped GDGTs, were present.The latter core lipids have been rarely reported previously.

View Article: PubMed Central - PubMed

Affiliation: Department of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands. schouten@nioz.nl

ABSTRACT
The lipid composition of Candidatus "Aciduliprofundum boonei", the only cultivated representative of archaea falling in the DHVE2 phylogenetic cluster, a group of microorganisms ubiquitously occurring at hydrothermal vents, was studied. The predominant core membrane lipids in this thermophilic euryarchaeote were found to be composed of glycerol dibiphytanyl glycerol tetraethers (GDGTs) containing 0-4 cyclopentyl moieties. In addition, GDGTs with an additional covalent bond between the isoprenoid hydrocarbon chains, so-called H-shaped GDGTs, were present. The latter core lipids have been rarely reported previously. Intact polar lipid analysis revealed that they predominantly consist of GDGTs with a phospho-glycerol headgroup.

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HPLC/APCI/MS base peak chromatogram of GDGT core lipids in extract released after base hydrolysis (using KOH/methanol mixture) of the cell material of Candidatus “Aciduliprofundum boonei”. Inset shows the atmospheric pressure chemical ionization mass spectrum of GDGT VII. Note that the position of the covalent bond between the isoprenoid hydrocarbon chains in GDGTs VII–XI is tentative (Morii et al. 1998)
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Fig1: HPLC/APCI/MS base peak chromatogram of GDGT core lipids in extract released after base hydrolysis (using KOH/methanol mixture) of the cell material of Candidatus “Aciduliprofundum boonei”. Inset shows the atmospheric pressure chemical ionization mass spectrum of GDGT VII. Note that the position of the covalent bond between the isoprenoid hydrocarbon chains in GDGTs VII–XI is tentative (Morii et al. 1998)

Mentions: Archaea are not only unique in their 16S rRNA phylogenetic position in the tree of life, but also synthesize specific membrane lipids. Analysis of cultivated hyperthermophilic Archaea showed that their membrane is predominantly composed of isoprenoid glycerol dibiphytanyl glycerol tetraethers (GDGTs) with additional cyclopentyl moieties (e.g. Structures I–VI in Fig. 1). The structural differences from diacyl membrane lipids of non-thermophilic Eukarya and Bacteria, i.e. ether bonds and the formation of a monolayer rather than a bilayer, have been suggested to contribute to the stability of membranes of hyperthermophiles at high temperatures and low pH (e.g. De Rosa and Gambacorta 1988; van den Vossenberg et al. 1998; Macalady et al. 2004). This suggests that members of the DHVE2 cluster may be also synthesizing GDGT membrane lipids. Therefore, in this study we analyzed the lipid composition of the only cultivated representative of DHVE2, Aciduliprofundum boonei, and examined both the core GDGT lipid composition as well as its intact polar lipid composition.Fig. 1


Tetraether membrane lipids of Candidatus "Aciduliprofundum boonei", a cultivated obligate thermoacidophilic euryarchaeote from deep-sea hydrothermal vents.

Schouten S, Baas M, Hopmans EC, Reysenbach AL, Damsté JS - Extremophiles (2007)

HPLC/APCI/MS base peak chromatogram of GDGT core lipids in extract released after base hydrolysis (using KOH/methanol mixture) of the cell material of Candidatus “Aciduliprofundum boonei”. Inset shows the atmospheric pressure chemical ionization mass spectrum of GDGT VII. Note that the position of the covalent bond between the isoprenoid hydrocarbon chains in GDGTs VII–XI is tentative (Morii et al. 1998)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2175526&req=5

Fig1: HPLC/APCI/MS base peak chromatogram of GDGT core lipids in extract released after base hydrolysis (using KOH/methanol mixture) of the cell material of Candidatus “Aciduliprofundum boonei”. Inset shows the atmospheric pressure chemical ionization mass spectrum of GDGT VII. Note that the position of the covalent bond between the isoprenoid hydrocarbon chains in GDGTs VII–XI is tentative (Morii et al. 1998)
Mentions: Archaea are not only unique in their 16S rRNA phylogenetic position in the tree of life, but also synthesize specific membrane lipids. Analysis of cultivated hyperthermophilic Archaea showed that their membrane is predominantly composed of isoprenoid glycerol dibiphytanyl glycerol tetraethers (GDGTs) with additional cyclopentyl moieties (e.g. Structures I–VI in Fig. 1). The structural differences from diacyl membrane lipids of non-thermophilic Eukarya and Bacteria, i.e. ether bonds and the formation of a monolayer rather than a bilayer, have been suggested to contribute to the stability of membranes of hyperthermophiles at high temperatures and low pH (e.g. De Rosa and Gambacorta 1988; van den Vossenberg et al. 1998; Macalady et al. 2004). This suggests that members of the DHVE2 cluster may be also synthesizing GDGT membrane lipids. Therefore, in this study we analyzed the lipid composition of the only cultivated representative of DHVE2, Aciduliprofundum boonei, and examined both the core GDGT lipid composition as well as its intact polar lipid composition.Fig. 1

Bottom Line: The predominant core membrane lipids in this thermophilic euryarchaeote were found to be composed of glycerol dibiphytanyl glycerol tetraethers (GDGTs) containing 0-4 cyclopentyl moieties.In addition, GDGTs with an additional covalent bond between the isoprenoid hydrocarbon chains, so-called H-shaped GDGTs, were present.The latter core lipids have been rarely reported previously.

View Article: PubMed Central - PubMed

Affiliation: Department of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands. schouten@nioz.nl

ABSTRACT
The lipid composition of Candidatus "Aciduliprofundum boonei", the only cultivated representative of archaea falling in the DHVE2 phylogenetic cluster, a group of microorganisms ubiquitously occurring at hydrothermal vents, was studied. The predominant core membrane lipids in this thermophilic euryarchaeote were found to be composed of glycerol dibiphytanyl glycerol tetraethers (GDGTs) containing 0-4 cyclopentyl moieties. In addition, GDGTs with an additional covalent bond between the isoprenoid hydrocarbon chains, so-called H-shaped GDGTs, were present. The latter core lipids have been rarely reported previously. Intact polar lipid analysis revealed that they predominantly consist of GDGTs with a phospho-glycerol headgroup.

Show MeSH
Related in: MedlinePlus