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Anopheles gambiae miRNAs as actors of defence reaction against Plasmodium invasion.

Winter F, Edaye S, Hüttenhofer A, Brunel C - Nucleic Acids Res. (2007)

Bottom Line: Twelve of them are expressed ubiquitously across the body, independently of gender, while the other six exhibited an expression pattern restricted to the digestive system.Strikingly, the expression patterns of four miRNAs, including the three unique to mosquito, are affected by the presence of Plasmodium.Altogether, these data support an involvement of miRNAs as new layers in the regulation of Anopheles defence reaction.

View Article: PubMed Central - PubMed

Affiliation: Architecture et Réactivité de l'ARN, Université Louis Pasteur, CNRS, Institut de Biologie Moléculaire et Cellulaire, 15 rue Descarte, 67084 Strasbourg, France.

ABSTRACT
The path Plasmodium takes across the Anopheles midgut constitutes the major bottleneck during the malaria transmission cycle. In the present study, using a combination of shot-gun cloning and bioinformatic analysis, we have identified 18 miRNAs from Anopheles gambiae including three miRNAs unique to mosquito. Twelve of them are expressed ubiquitously across the body, independently of gender, while the other six exhibited an expression pattern restricted to the digestive system. Strikingly, the expression patterns of four miRNAs, including the three unique to mosquito, are affected by the presence of Plasmodium. We also show that knocking down Dicer1 and Ago1 mRNAs led to an increased sensitivity to Plasmodium infection. Altogether, these data support an involvement of miRNAs as new layers in the regulation of Anopheles defence reaction.

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Cluster organization. The symbol (*, asterisk) indicates the miRNA cloned in this study. Exons are symbolized using broken boxes. Position of the mature miRNA within its stem-loop precursor is indicated using a darker line. Additional information is gathered in Supplementary Data (Table S2).
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Figure 1: Cluster organization. The symbol (*, asterisk) indicates the miRNA cloned in this study. Exons are symbolized using broken boxes. Position of the mature miRNA within its stem-loop precursor is indicated using a darker line. Additional information is gathered in Supplementary Data (Table S2).

Mentions: Ten cloned sequences met all the criteria to be bona fide miRNAs (Table 1). We identified eigth additional A. gambiae miRNAs employing the synteny of miRNA gene clustering (Figure 1 and Supplementary Data, Table S2).Figure 1.


Anopheles gambiae miRNAs as actors of defence reaction against Plasmodium invasion.

Winter F, Edaye S, Hüttenhofer A, Brunel C - Nucleic Acids Res. (2007)

Cluster organization. The symbol (*, asterisk) indicates the miRNA cloned in this study. Exons are symbolized using broken boxes. Position of the mature miRNA within its stem-loop precursor is indicated using a darker line. Additional information is gathered in Supplementary Data (Table S2).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2175301&req=5

Figure 1: Cluster organization. The symbol (*, asterisk) indicates the miRNA cloned in this study. Exons are symbolized using broken boxes. Position of the mature miRNA within its stem-loop precursor is indicated using a darker line. Additional information is gathered in Supplementary Data (Table S2).
Mentions: Ten cloned sequences met all the criteria to be bona fide miRNAs (Table 1). We identified eigth additional A. gambiae miRNAs employing the synteny of miRNA gene clustering (Figure 1 and Supplementary Data, Table S2).Figure 1.

Bottom Line: Twelve of them are expressed ubiquitously across the body, independently of gender, while the other six exhibited an expression pattern restricted to the digestive system.Strikingly, the expression patterns of four miRNAs, including the three unique to mosquito, are affected by the presence of Plasmodium.Altogether, these data support an involvement of miRNAs as new layers in the regulation of Anopheles defence reaction.

View Article: PubMed Central - PubMed

Affiliation: Architecture et Réactivité de l'ARN, Université Louis Pasteur, CNRS, Institut de Biologie Moléculaire et Cellulaire, 15 rue Descarte, 67084 Strasbourg, France.

ABSTRACT
The path Plasmodium takes across the Anopheles midgut constitutes the major bottleneck during the malaria transmission cycle. In the present study, using a combination of shot-gun cloning and bioinformatic analysis, we have identified 18 miRNAs from Anopheles gambiae including three miRNAs unique to mosquito. Twelve of them are expressed ubiquitously across the body, independently of gender, while the other six exhibited an expression pattern restricted to the digestive system. Strikingly, the expression patterns of four miRNAs, including the three unique to mosquito, are affected by the presence of Plasmodium. We also show that knocking down Dicer1 and Ago1 mRNAs led to an increased sensitivity to Plasmodium infection. Altogether, these data support an involvement of miRNAs as new layers in the regulation of Anopheles defence reaction.

Show MeSH
Related in: MedlinePlus