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Inheritance of gene density-related higher order chromatin arrangements in normal and tumor cell nuclei.

Cremer M, Küpper K, Wagler B, Wizelman L, von Hase J, Weiland Y, Kreja L, Diebold J, Speicher MR, Cremer T - J. Cell Biol. (2003)

Bottom Line: Bickmore. 1999.Cell Biol. 145:1119-1131).Our findings demonstrate that a significant difference in the radial distribution of #18 and #19 chromatin is a common feature of higher order chromatin architecture in both normal and malignant cell types.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology II, Ludwig Maximilians University, 80333 Munich, Germany. Marion.Cremer@lrz.uni-muenchen.de

ABSTRACT
A gene density-related difference in the radial arrangement of chromosome territories (CTs) was previously described for human lymphocyte nuclei with gene-poor CT #18 located toward the nuclear periphery and gene-dense CT #19 in the nuclear interior (Croft, J.A., J.M. Bridger, S. Boyle, P. Perry, P. Teague, and W.A. Bickmore. 1999. J. Cell Biol. 145:1119-1131). Here, we analyzed the radial distribution of chromosome 18 and 19 chromatin in six normal cell types and in eight tumor cell lines, some of them with imbalances and rearrangements of the two chromosomes. Our findings demonstrate that a significant difference in the radial distribution of #18 and #19 chromatin is a common feature of higher order chromatin architecture in both normal and malignant cell types. However, in seven of eight tumor cell lines, the difference was less pronounced compared with normal cell nuclei due to a higher fraction of nuclei showing an inverted CT position, i.e., a CT #18 located more internally than a CT #19. This observation emphasizes a partial loss of radial chromatin order in tumor cell nuclei.

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Copy numbers of chromosome segments estimated by CGH analysis, considering ploidy information of the karyotypes from each cell line. Each bar represents the chromosome copy number in a particular cell line; different copy numbers are represented by different colors according to the key shown. Cell lines from left (nearest to chromosome ideogram) to right are: (1) Jurkat; (2) HDLM-2; (3) MelJuso; (4) HeLa; (5) SW480; (6) SW620 (furthest from chromosome ideogram). Note that the HeLa cell line is near triploid. Accordingly, the loss of material (i.e., chromosomes 4 and 18q) indicates a disomic status (compare with Table I).
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fig1: Copy numbers of chromosome segments estimated by CGH analysis, considering ploidy information of the karyotypes from each cell line. Each bar represents the chromosome copy number in a particular cell line; different copy numbers are represented by different colors according to the key shown. Cell lines from left (nearest to chromosome ideogram) to right are: (1) Jurkat; (2) HDLM-2; (3) MelJuso; (4) HeLa; (5) SW480; (6) SW620 (furthest from chromosome ideogram). Note that the HeLa cell line is near triploid. Accordingly, the loss of material (i.e., chromosomes 4 and 18q) indicates a disomic status (compare with Table I).

Mentions: A short description of all normal cell types and tumor cell lines analyzed in this report, frequent structural aberrations, as well as copy number changes concerning chromosomes 18 and 19 are summarized in Table I. In cases where karyotype information on rearranged cell lines with heterogenic karyotype was based on data reported by other groups (Jurkat, HDLM-2, MelJuso, SW480, and SW620) we took care to perform our 3D-FISH analyses on nuclei from a cell passage that our providers had stored close to that used for karyotype analysis. Data on the copy number of chromosome segments were obtained by comparative genomic hybridization (CGH) analysis for each cell line with major chromosomal rearrangements, considering ploidy information from the karyotype (Fig. 1). The DNA, used for CGH analysis, was also obtained from a cell passage close to that used for 3D-FISH. Data for the assessment of the nuclear shape are listed in Table II.


Inheritance of gene density-related higher order chromatin arrangements in normal and tumor cell nuclei.

Cremer M, Küpper K, Wagler B, Wizelman L, von Hase J, Weiland Y, Kreja L, Diebold J, Speicher MR, Cremer T - J. Cell Biol. (2003)

Copy numbers of chromosome segments estimated by CGH analysis, considering ploidy information of the karyotypes from each cell line. Each bar represents the chromosome copy number in a particular cell line; different copy numbers are represented by different colors according to the key shown. Cell lines from left (nearest to chromosome ideogram) to right are: (1) Jurkat; (2) HDLM-2; (3) MelJuso; (4) HeLa; (5) SW480; (6) SW620 (furthest from chromosome ideogram). Note that the HeLa cell line is near triploid. Accordingly, the loss of material (i.e., chromosomes 4 and 18q) indicates a disomic status (compare with Table I).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2172812&req=5

fig1: Copy numbers of chromosome segments estimated by CGH analysis, considering ploidy information of the karyotypes from each cell line. Each bar represents the chromosome copy number in a particular cell line; different copy numbers are represented by different colors according to the key shown. Cell lines from left (nearest to chromosome ideogram) to right are: (1) Jurkat; (2) HDLM-2; (3) MelJuso; (4) HeLa; (5) SW480; (6) SW620 (furthest from chromosome ideogram). Note that the HeLa cell line is near triploid. Accordingly, the loss of material (i.e., chromosomes 4 and 18q) indicates a disomic status (compare with Table I).
Mentions: A short description of all normal cell types and tumor cell lines analyzed in this report, frequent structural aberrations, as well as copy number changes concerning chromosomes 18 and 19 are summarized in Table I. In cases where karyotype information on rearranged cell lines with heterogenic karyotype was based on data reported by other groups (Jurkat, HDLM-2, MelJuso, SW480, and SW620) we took care to perform our 3D-FISH analyses on nuclei from a cell passage that our providers had stored close to that used for karyotype analysis. Data on the copy number of chromosome segments were obtained by comparative genomic hybridization (CGH) analysis for each cell line with major chromosomal rearrangements, considering ploidy information from the karyotype (Fig. 1). The DNA, used for CGH analysis, was also obtained from a cell passage close to that used for 3D-FISH. Data for the assessment of the nuclear shape are listed in Table II.

Bottom Line: Bickmore. 1999.Cell Biol. 145:1119-1131).Our findings demonstrate that a significant difference in the radial distribution of #18 and #19 chromatin is a common feature of higher order chromatin architecture in both normal and malignant cell types.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology II, Ludwig Maximilians University, 80333 Munich, Germany. Marion.Cremer@lrz.uni-muenchen.de

ABSTRACT
A gene density-related difference in the radial arrangement of chromosome territories (CTs) was previously described for human lymphocyte nuclei with gene-poor CT #18 located toward the nuclear periphery and gene-dense CT #19 in the nuclear interior (Croft, J.A., J.M. Bridger, S. Boyle, P. Perry, P. Teague, and W.A. Bickmore. 1999. J. Cell Biol. 145:1119-1131). Here, we analyzed the radial distribution of chromosome 18 and 19 chromatin in six normal cell types and in eight tumor cell lines, some of them with imbalances and rearrangements of the two chromosomes. Our findings demonstrate that a significant difference in the radial distribution of #18 and #19 chromatin is a common feature of higher order chromatin architecture in both normal and malignant cell types. However, in seven of eight tumor cell lines, the difference was less pronounced compared with normal cell nuclei due to a higher fraction of nuclei showing an inverted CT position, i.e., a CT #18 located more internally than a CT #19. This observation emphasizes a partial loss of radial chromatin order in tumor cell nuclei.

Show MeSH
Related in: MedlinePlus