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Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells.

De Bellard ME, Rao Y, Bronner-Fraser M - J. Cell Biol. (2003)

Bottom Line: Accordingly, only trunk neural crest cells express Robo receptors.Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells.These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.

ABSTRACT
Neural crest precursors to the autonomic nervous system form different derivatives depending upon their axial level of origin; for example, vagal, but not trunk, neural crest cells form the enteric ganglia of the gut. Here, we show that Slit2 is expressed at the entrance of the gut, which is selectively invaded by vagal, but not trunk, neural crest. Accordingly, only trunk neural crest cells express Robo receptors. In vivo and in vitro experiments demonstrate that trunk, not vagal, crest cells avoid cells or cell membranes expressing Slit2, thereby contributing to the differential ability of neural crest populations to invade and innervate the gut. Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells. These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.

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Movie stills from time-lapse video microscopy. Trunk neural crest cells exposed to Slit2 migrate further and have a longer total path length than those exposed to control medium. Trunk neural crest cells were labeled with Calcein AM (Molecular Probes) and washed before exposure to control or Slit2 CM. Cultures were time lapsed for ∼2.5 h under a confocal microscope. Images represent stills from a movie taken at the indicated times. Two cells (red) in each movie were manually traced to follow their movements. Their final path length is indicated in yellow in the last frame.
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fig8: Movie stills from time-lapse video microscopy. Trunk neural crest cells exposed to Slit2 migrate further and have a longer total path length than those exposed to control medium. Trunk neural crest cells were labeled with Calcein AM (Molecular Probes) and washed before exposure to control or Slit2 CM. Cultures were time lapsed for ∼2.5 h under a confocal microscope. Images represent stills from a movie taken at the indicated times. Two cells (red) in each movie were manually traced to follow their movements. Their final path length is indicated in yellow in the last frame.

Mentions: The results confirmed static pictures showing that trunk neural crest cells moved more dynamically and for greater distances in Slit2 than in control medium (Fig. 8; see Videos 1 and 2, available at http://www.jcb.org/cgi/content/full/jcb.200301041/DC1). With or without Slit2, neural crest cells moved in a somewhat erratic manner, with frequent changes in direction (Kulesa and Fraser, 1998, 2000). The cells appeared to collide more frequently in the presence of Slit2, raising the possibility that cell–cell collision may effect their degree of movement.


Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells.

De Bellard ME, Rao Y, Bronner-Fraser M - J. Cell Biol. (2003)

Movie stills from time-lapse video microscopy. Trunk neural crest cells exposed to Slit2 migrate further and have a longer total path length than those exposed to control medium. Trunk neural crest cells were labeled with Calcein AM (Molecular Probes) and washed before exposure to control or Slit2 CM. Cultures were time lapsed for ∼2.5 h under a confocal microscope. Images represent stills from a movie taken at the indicated times. Two cells (red) in each movie were manually traced to follow their movements. Their final path length is indicated in yellow in the last frame.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2172792&req=5

fig8: Movie stills from time-lapse video microscopy. Trunk neural crest cells exposed to Slit2 migrate further and have a longer total path length than those exposed to control medium. Trunk neural crest cells were labeled with Calcein AM (Molecular Probes) and washed before exposure to control or Slit2 CM. Cultures were time lapsed for ∼2.5 h under a confocal microscope. Images represent stills from a movie taken at the indicated times. Two cells (red) in each movie were manually traced to follow their movements. Their final path length is indicated in yellow in the last frame.
Mentions: The results confirmed static pictures showing that trunk neural crest cells moved more dynamically and for greater distances in Slit2 than in control medium (Fig. 8; see Videos 1 and 2, available at http://www.jcb.org/cgi/content/full/jcb.200301041/DC1). With or without Slit2, neural crest cells moved in a somewhat erratic manner, with frequent changes in direction (Kulesa and Fraser, 1998, 2000). The cells appeared to collide more frequently in the presence of Slit2, raising the possibility that cell–cell collision may effect their degree of movement.

Bottom Line: Accordingly, only trunk neural crest cells express Robo receptors.Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells.These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.

ABSTRACT
Neural crest precursors to the autonomic nervous system form different derivatives depending upon their axial level of origin; for example, vagal, but not trunk, neural crest cells form the enteric ganglia of the gut. Here, we show that Slit2 is expressed at the entrance of the gut, which is selectively invaded by vagal, but not trunk, neural crest. Accordingly, only trunk neural crest cells express Robo receptors. In vivo and in vitro experiments demonstrate that trunk, not vagal, crest cells avoid cells or cell membranes expressing Slit2, thereby contributing to the differential ability of neural crest populations to invade and innervate the gut. Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells. These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.

Show MeSH
Related in: MedlinePlus