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Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells.

De Bellard ME, Rao Y, Bronner-Fraser M - J. Cell Biol. (2003)

Bottom Line: Accordingly, only trunk neural crest cells express Robo receptors.Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells.These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.

ABSTRACT
Neural crest precursors to the autonomic nervous system form different derivatives depending upon their axial level of origin; for example, vagal, but not trunk, neural crest cells form the enteric ganglia of the gut. Here, we show that Slit2 is expressed at the entrance of the gut, which is selectively invaded by vagal, but not trunk, neural crest. Accordingly, only trunk neural crest cells express Robo receptors. In vivo and in vitro experiments demonstrate that trunk, not vagal, crest cells avoid cells or cell membranes expressing Slit2, thereby contributing to the differential ability of neural crest populations to invade and innervate the gut. Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells. These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.

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Neural crest cells migrate further in the presence of Slit2 CM. (a) A neural tube explanted in the presence of CM from control cells shows that migrating neural crest cells have moved several cell diameters away from the neural tube after 18 h in culture. (b) A similar neural tube cultured in medium conditioned by Slit2-expressing cells had neural crest cells that had migrated significantly further in 18 h.
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fig6: Neural crest cells migrate further in the presence of Slit2 CM. (a) A neural tube explanted in the presence of CM from control cells shows that migrating neural crest cells have moved several cell diameters away from the neural tube after 18 h in culture. (b) A similar neural tube cultured in medium conditioned by Slit2-expressing cells had neural crest cells that had migrated significantly further in 18 h.

Mentions: To examine the effects of soluble Slit2, migrating neural crest cells were placed on fibronectin-coated substrates transfilter to Slit2-expressing cells, control cells, Slit2-conditioned medium (CM),* or control CM (Wu et al., 2001). This assures that crest cells under experimental conditions would be exposed only to the soluble form of Slit2. With either transfilter Slit2 cells or Slit2 CM, the maximum distance traveled by neural crest cells was an average of 21% more than that observed under control conditions (n > 100 neural tubes in a total of 12 separate experiments; Table III; Fig. 6). In contrast, vagal neural crest cells failed to exhibit enhanced migration under identical conditions (n = 12 neural tubes in a total of three separate experiments; Table III).


Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells.

De Bellard ME, Rao Y, Bronner-Fraser M - J. Cell Biol. (2003)

Neural crest cells migrate further in the presence of Slit2 CM. (a) A neural tube explanted in the presence of CM from control cells shows that migrating neural crest cells have moved several cell diameters away from the neural tube after 18 h in culture. (b) A similar neural tube cultured in medium conditioned by Slit2-expressing cells had neural crest cells that had migrated significantly further in 18 h.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2172792&req=5

fig6: Neural crest cells migrate further in the presence of Slit2 CM. (a) A neural tube explanted in the presence of CM from control cells shows that migrating neural crest cells have moved several cell diameters away from the neural tube after 18 h in culture. (b) A similar neural tube cultured in medium conditioned by Slit2-expressing cells had neural crest cells that had migrated significantly further in 18 h.
Mentions: To examine the effects of soluble Slit2, migrating neural crest cells were placed on fibronectin-coated substrates transfilter to Slit2-expressing cells, control cells, Slit2-conditioned medium (CM),* or control CM (Wu et al., 2001). This assures that crest cells under experimental conditions would be exposed only to the soluble form of Slit2. With either transfilter Slit2 cells or Slit2 CM, the maximum distance traveled by neural crest cells was an average of 21% more than that observed under control conditions (n > 100 neural tubes in a total of 12 separate experiments; Table III; Fig. 6). In contrast, vagal neural crest cells failed to exhibit enhanced migration under identical conditions (n = 12 neural tubes in a total of three separate experiments; Table III).

Bottom Line: Accordingly, only trunk neural crest cells express Robo receptors.Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells.These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.

ABSTRACT
Neural crest precursors to the autonomic nervous system form different derivatives depending upon their axial level of origin; for example, vagal, but not trunk, neural crest cells form the enteric ganglia of the gut. Here, we show that Slit2 is expressed at the entrance of the gut, which is selectively invaded by vagal, but not trunk, neural crest. Accordingly, only trunk neural crest cells express Robo receptors. In vivo and in vitro experiments demonstrate that trunk, not vagal, crest cells avoid cells or cell membranes expressing Slit2, thereby contributing to the differential ability of neural crest populations to invade and innervate the gut. Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells. These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.

Show MeSH
Related in: MedlinePlus