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Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells.

De Bellard ME, Rao Y, Bronner-Fraser M - J. Cell Biol. (2003)

Bottom Line: Accordingly, only trunk neural crest cells express Robo receptors.Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells.These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.

ABSTRACT
Neural crest precursors to the autonomic nervous system form different derivatives depending upon their axial level of origin; for example, vagal, but not trunk, neural crest cells form the enteric ganglia of the gut. Here, we show that Slit2 is expressed at the entrance of the gut, which is selectively invaded by vagal, but not trunk, neural crest. Accordingly, only trunk neural crest cells express Robo receptors. In vivo and in vitro experiments demonstrate that trunk, not vagal, crest cells avoid cells or cell membranes expressing Slit2, thereby contributing to the differential ability of neural crest populations to invade and innervate the gut. Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells. These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.

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Trunk, not vagal, neural crest cells are repelled by Slit2- expressing cells in vitro. (a–d) Trunk and vagal neural crest cells (green) were grown apposed to live control HEK cells (a and c) or Slit2-expressing cells (b and d) (blue DAPI label). Only when trunk neural crest cells (b) were grown with Slit2-expressing cells was there a sharp border (white arrowhead) formed between the two populations. (e–h) A similar experiment performed with dead control or Slit2-expressing cells again shows a border between trunk neural crest cells and the Slit cell ghosts (white arrowhead in f), demonstrating that the repellent activity is membrane bound.
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fig5: Trunk, not vagal, neural crest cells are repelled by Slit2- expressing cells in vitro. (a–d) Trunk and vagal neural crest cells (green) were grown apposed to live control HEK cells (a and c) or Slit2-expressing cells (b and d) (blue DAPI label). Only when trunk neural crest cells (b) were grown with Slit2-expressing cells was there a sharp border (white arrowhead) formed between the two populations. (e–h) A similar experiment performed with dead control or Slit2-expressing cells again shows a border between trunk neural crest cells and the Slit cell ghosts (white arrowhead in f), demonstrating that the repellent activity is membrane bound.

Mentions: Whereas trunk neural crest cells freely approached and intermixed with control HEK cells (Fig. 5 a), a sharp border was observed between trunk neural crest cells and Slit2-expressing cells (Fig. 5 b). A similar experiment was performed for vagal neural crest cells, using neural tubes explanted from the levels of somites 1–7 of stage 10–12 embryos. In this case, the vagal cells intermixed equally well with Slit2-expressing cells and control cells (Figs. 5, c and d). Thus, analogous differences in the response of trunk versus vagal neural crest cells were noted both in vivo and in vitro, suggesting that the former, but not the latter, avoided an exogenous source of Slit2. Table I summarizes the results for in vivo and in vitro assays.


Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells.

De Bellard ME, Rao Y, Bronner-Fraser M - J. Cell Biol. (2003)

Trunk, not vagal, neural crest cells are repelled by Slit2- expressing cells in vitro. (a–d) Trunk and vagal neural crest cells (green) were grown apposed to live control HEK cells (a and c) or Slit2-expressing cells (b and d) (blue DAPI label). Only when trunk neural crest cells (b) were grown with Slit2-expressing cells was there a sharp border (white arrowhead) formed between the two populations. (e–h) A similar experiment performed with dead control or Slit2-expressing cells again shows a border between trunk neural crest cells and the Slit cell ghosts (white arrowhead in f), demonstrating that the repellent activity is membrane bound.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2172792&req=5

fig5: Trunk, not vagal, neural crest cells are repelled by Slit2- expressing cells in vitro. (a–d) Trunk and vagal neural crest cells (green) were grown apposed to live control HEK cells (a and c) or Slit2-expressing cells (b and d) (blue DAPI label). Only when trunk neural crest cells (b) were grown with Slit2-expressing cells was there a sharp border (white arrowhead) formed between the two populations. (e–h) A similar experiment performed with dead control or Slit2-expressing cells again shows a border between trunk neural crest cells and the Slit cell ghosts (white arrowhead in f), demonstrating that the repellent activity is membrane bound.
Mentions: Whereas trunk neural crest cells freely approached and intermixed with control HEK cells (Fig. 5 a), a sharp border was observed between trunk neural crest cells and Slit2-expressing cells (Fig. 5 b). A similar experiment was performed for vagal neural crest cells, using neural tubes explanted from the levels of somites 1–7 of stage 10–12 embryos. In this case, the vagal cells intermixed equally well with Slit2-expressing cells and control cells (Figs. 5, c and d). Thus, analogous differences in the response of trunk versus vagal neural crest cells were noted both in vivo and in vitro, suggesting that the former, but not the latter, avoided an exogenous source of Slit2. Table I summarizes the results for in vivo and in vitro assays.

Bottom Line: Accordingly, only trunk neural crest cells express Robo receptors.Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells.These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.

ABSTRACT
Neural crest precursors to the autonomic nervous system form different derivatives depending upon their axial level of origin; for example, vagal, but not trunk, neural crest cells form the enteric ganglia of the gut. Here, we show that Slit2 is expressed at the entrance of the gut, which is selectively invaded by vagal, but not trunk, neural crest. Accordingly, only trunk neural crest cells express Robo receptors. In vivo and in vitro experiments demonstrate that trunk, not vagal, crest cells avoid cells or cell membranes expressing Slit2, thereby contributing to the differential ability of neural crest populations to invade and innervate the gut. Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells. These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.

Show MeSH
Related in: MedlinePlus