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Dual regulation of neuronal morphogenesis by a delta-catenin-cortactin complex and Rho.

Martinez MC, Ochiishi T, Majewski M, Kosik KS - J. Cell Biol. (2003)

Bottom Line: Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes.When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching.We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Neurology, Brigham and Women's Hospital and Harvard Medical School, Harvard Institute of Medicine, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

ABSTRACT
Delta-catenin is a neuronal protein that contains 10 Armadillo motifs and binds to the juxtamembrane segment of classical cadherins. We report that delta-catenin interacts with cortactin in a tyrosine phosphorylation-dependent manner. This interaction occurs within a region of the delta-catenin sequence that is also essential for the neurite elongation effects. Src family kinases can phosphorylate delta-catenin and bind to delta-catenin through its polyproline tract. Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes. Conversely, increasing tyrosine phosphorylation disrupts the delta-catenin-cortactin complex. When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching. We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

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The COOH terminus of δ-catenin is necessary for neurite formation. (A) Schematic representation of δ-catenin and cortactin deletion constructs. (a) δ-Catenin deletion constructs fused to GFP (as described in Materials and methods). Constructs are named by the number of amino acids deleted from the COOH terminus. (b) Schematic representation of the cortactin GST fusion constructs. (B) Hippocampal neurons in culture for 8 d (a) and NGF-treated PC12 cells (b) were transfected with the GFP, FLδ, ΔC205, and ΔC99 constructs. Quantification of the number and length of protrusions for the hippocampal neurons and the number of primary branches and total summed length of all processes for PC12 cells are shown.
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fig3: The COOH terminus of δ-catenin is necessary for neurite formation. (A) Schematic representation of δ-catenin and cortactin deletion constructs. (a) δ-Catenin deletion constructs fused to GFP (as described in Materials and methods). Constructs are named by the number of amino acids deleted from the COOH terminus. (b) Schematic representation of the cortactin GST fusion constructs. (B) Hippocampal neurons in culture for 8 d (a) and NGF-treated PC12 cells (b) were transfected with the GFP, FLδ, ΔC205, and ΔC99 constructs. Quantification of the number and length of protrusions for the hippocampal neurons and the number of primary branches and total summed length of all processes for PC12 cells are shown.

Mentions: δ-Catenin deletion constructs (Fig. 3Figure 3.


Dual regulation of neuronal morphogenesis by a delta-catenin-cortactin complex and Rho.

Martinez MC, Ochiishi T, Majewski M, Kosik KS - J. Cell Biol. (2003)

The COOH terminus of δ-catenin is necessary for neurite formation. (A) Schematic representation of δ-catenin and cortactin deletion constructs. (a) δ-Catenin deletion constructs fused to GFP (as described in Materials and methods). Constructs are named by the number of amino acids deleted from the COOH terminus. (b) Schematic representation of the cortactin GST fusion constructs. (B) Hippocampal neurons in culture for 8 d (a) and NGF-treated PC12 cells (b) were transfected with the GFP, FLδ, ΔC205, and ΔC99 constructs. Quantification of the number and length of protrusions for the hippocampal neurons and the number of primary branches and total summed length of all processes for PC12 cells are shown.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2172717&req=5

fig3: The COOH terminus of δ-catenin is necessary for neurite formation. (A) Schematic representation of δ-catenin and cortactin deletion constructs. (a) δ-Catenin deletion constructs fused to GFP (as described in Materials and methods). Constructs are named by the number of amino acids deleted from the COOH terminus. (b) Schematic representation of the cortactin GST fusion constructs. (B) Hippocampal neurons in culture for 8 d (a) and NGF-treated PC12 cells (b) were transfected with the GFP, FLδ, ΔC205, and ΔC99 constructs. Quantification of the number and length of protrusions for the hippocampal neurons and the number of primary branches and total summed length of all processes for PC12 cells are shown.
Mentions: δ-Catenin deletion constructs (Fig. 3Figure 3.

Bottom Line: Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes.When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching.We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Neurology, Brigham and Women's Hospital and Harvard Medical School, Harvard Institute of Medicine, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

ABSTRACT
Delta-catenin is a neuronal protein that contains 10 Armadillo motifs and binds to the juxtamembrane segment of classical cadherins. We report that delta-catenin interacts with cortactin in a tyrosine phosphorylation-dependent manner. This interaction occurs within a region of the delta-catenin sequence that is also essential for the neurite elongation effects. Src family kinases can phosphorylate delta-catenin and bind to delta-catenin through its polyproline tract. Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes. Conversely, increasing tyrosine phosphorylation disrupts the delta-catenin-cortactin complex. When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching. We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

Show MeSH
Related in: MedlinePlus