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Dual regulation of neuronal morphogenesis by a delta-catenin-cortactin complex and Rho.

Martinez MC, Ochiishi T, Majewski M, Kosik KS - J. Cell Biol. (2003)

Bottom Line: Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes.When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching.We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Neurology, Brigham and Women's Hospital and Harvard Medical School, Harvard Institute of Medicine, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

ABSTRACT
Delta-catenin is a neuronal protein that contains 10 Armadillo motifs and binds to the juxtamembrane segment of classical cadherins. We report that delta-catenin interacts with cortactin in a tyrosine phosphorylation-dependent manner. This interaction occurs within a region of the delta-catenin sequence that is also essential for the neurite elongation effects. Src family kinases can phosphorylate delta-catenin and bind to delta-catenin through its polyproline tract. Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes. Conversely, increasing tyrosine phosphorylation disrupts the delta-catenin-cortactin complex. When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching. We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

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Related in: MedlinePlus

δ-Catenin induces protrusions along the distal dendritic shafts of hippocampal neurons. (A) Rat hippocampal neurons in culture for 8 d were transfected with GFP (a) and GFP–δ-catenin constructs (b) and fixed 24 h later. Small branches and filopodial-like protrusions on dendrites are indicated by arrows in b. Bar, 15 μm. (B) Hippocampal neurons in culture for 8 d were transfected with GFP–δ-catenin (d–f) and stained for MAP2 (a), actin (b), and tubulin (c). Panels g–i show colocalization. Bar, 5 μm. (C) Quantification of the number and length of protrusions for GFP and GFP–δ-catenin–transfected neurons. Detail of the protrusions is shown for GFP- (a) and GFP–δ-catenin– (b) transfected neurons. Bar, 5 μm.
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fig2: δ-Catenin induces protrusions along the distal dendritic shafts of hippocampal neurons. (A) Rat hippocampal neurons in culture for 8 d were transfected with GFP (a) and GFP–δ-catenin constructs (b) and fixed 24 h later. Small branches and filopodial-like protrusions on dendrites are indicated by arrows in b. Bar, 15 μm. (B) Hippocampal neurons in culture for 8 d were transfected with GFP–δ-catenin (d–f) and stained for MAP2 (a), actin (b), and tubulin (c). Panels g–i show colocalization. Bar, 5 μm. (C) Quantification of the number and length of protrusions for GFP and GFP–δ-catenin–transfected neurons. Detail of the protrusions is shown for GFP- (a) and GFP–δ-catenin– (b) transfected neurons. Bar, 5 μm.

Mentions: Transfection of δ-catenin into cultured hippocampal neurons at 9 d in vitro also alters cell morphology (Fig. 2Figure 2.


Dual regulation of neuronal morphogenesis by a delta-catenin-cortactin complex and Rho.

Martinez MC, Ochiishi T, Majewski M, Kosik KS - J. Cell Biol. (2003)

δ-Catenin induces protrusions along the distal dendritic shafts of hippocampal neurons. (A) Rat hippocampal neurons in culture for 8 d were transfected with GFP (a) and GFP–δ-catenin constructs (b) and fixed 24 h later. Small branches and filopodial-like protrusions on dendrites are indicated by arrows in b. Bar, 15 μm. (B) Hippocampal neurons in culture for 8 d were transfected with GFP–δ-catenin (d–f) and stained for MAP2 (a), actin (b), and tubulin (c). Panels g–i show colocalization. Bar, 5 μm. (C) Quantification of the number and length of protrusions for GFP and GFP–δ-catenin–transfected neurons. Detail of the protrusions is shown for GFP- (a) and GFP–δ-catenin– (b) transfected neurons. Bar, 5 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2172717&req=5

fig2: δ-Catenin induces protrusions along the distal dendritic shafts of hippocampal neurons. (A) Rat hippocampal neurons in culture for 8 d were transfected with GFP (a) and GFP–δ-catenin constructs (b) and fixed 24 h later. Small branches and filopodial-like protrusions on dendrites are indicated by arrows in b. Bar, 15 μm. (B) Hippocampal neurons in culture for 8 d were transfected with GFP–δ-catenin (d–f) and stained for MAP2 (a), actin (b), and tubulin (c). Panels g–i show colocalization. Bar, 5 μm. (C) Quantification of the number and length of protrusions for GFP and GFP–δ-catenin–transfected neurons. Detail of the protrusions is shown for GFP- (a) and GFP–δ-catenin– (b) transfected neurons. Bar, 5 μm.
Mentions: Transfection of δ-catenin into cultured hippocampal neurons at 9 d in vitro also alters cell morphology (Fig. 2Figure 2.

Bottom Line: Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes.When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching.We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Neurology, Brigham and Women's Hospital and Harvard Medical School, Harvard Institute of Medicine, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

ABSTRACT
Delta-catenin is a neuronal protein that contains 10 Armadillo motifs and binds to the juxtamembrane segment of classical cadherins. We report that delta-catenin interacts with cortactin in a tyrosine phosphorylation-dependent manner. This interaction occurs within a region of the delta-catenin sequence that is also essential for the neurite elongation effects. Src family kinases can phosphorylate delta-catenin and bind to delta-catenin through its polyproline tract. Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes. Conversely, increasing tyrosine phosphorylation disrupts the delta-catenin-cortactin complex. When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching. We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

Show MeSH
Related in: MedlinePlus