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Dual regulation of neuronal morphogenesis by a delta-catenin-cortactin complex and Rho.

Martinez MC, Ochiishi T, Majewski M, Kosik KS - J. Cell Biol. (2003)

Bottom Line: Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes.When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching.We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Neurology, Brigham and Women's Hospital and Harvard Medical School, Harvard Institute of Medicine, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

ABSTRACT
Delta-catenin is a neuronal protein that contains 10 Armadillo motifs and binds to the juxtamembrane segment of classical cadherins. We report that delta-catenin interacts with cortactin in a tyrosine phosphorylation-dependent manner. This interaction occurs within a region of the delta-catenin sequence that is also essential for the neurite elongation effects. Src family kinases can phosphorylate delta-catenin and bind to delta-catenin through its polyproline tract. Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes. Conversely, increasing tyrosine phosphorylation disrupts the delta-catenin-cortactin complex. When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching. We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

Show MeSH
δ-Catenin and primary process extension versus branching. Two different pathways regulate the effects of δ-catenin on process elaboration. Extracellular signals such as neurotrophins acting through regulation of Src family kinases can lead to δ-catenin–cortactin complex formation. The complex becomes competent to participate in primary process elongation by recruiting the Arp2/3 complex. Rho inhibition can be amplified by δ-catenin, which leads to branching.
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fig10: δ-Catenin and primary process extension versus branching. Two different pathways regulate the effects of δ-catenin on process elaboration. Extracellular signals such as neurotrophins acting through regulation of Src family kinases can lead to δ-catenin–cortactin complex formation. The complex becomes competent to participate in primary process elongation by recruiting the Arp2/3 complex. Rho inhibition can be amplified by δ-catenin, which leads to branching.

Mentions: A balance between process elongation and branching is critical for the cell to achieve its ultimate shape. These two morphologic phenotypes may operate by distinct but not necessarily autonomous signaling pathways. Extensively branched processes regulated by Rho or primary process extension with minimal secondary branching regulated by Src are both induced in association with δ-catenin expression (Fig. 10)Figure 10.


Dual regulation of neuronal morphogenesis by a delta-catenin-cortactin complex and Rho.

Martinez MC, Ochiishi T, Majewski M, Kosik KS - J. Cell Biol. (2003)

δ-Catenin and primary process extension versus branching. Two different pathways regulate the effects of δ-catenin on process elaboration. Extracellular signals such as neurotrophins acting through regulation of Src family kinases can lead to δ-catenin–cortactin complex formation. The complex becomes competent to participate in primary process elongation by recruiting the Arp2/3 complex. Rho inhibition can be amplified by δ-catenin, which leads to branching.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2172717&req=5

fig10: δ-Catenin and primary process extension versus branching. Two different pathways regulate the effects of δ-catenin on process elaboration. Extracellular signals such as neurotrophins acting through regulation of Src family kinases can lead to δ-catenin–cortactin complex formation. The complex becomes competent to participate in primary process elongation by recruiting the Arp2/3 complex. Rho inhibition can be amplified by δ-catenin, which leads to branching.
Mentions: A balance between process elongation and branching is critical for the cell to achieve its ultimate shape. These two morphologic phenotypes may operate by distinct but not necessarily autonomous signaling pathways. Extensively branched processes regulated by Rho or primary process extension with minimal secondary branching regulated by Src are both induced in association with δ-catenin expression (Fig. 10)Figure 10.

Bottom Line: Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes.When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching.We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Neurology, Brigham and Women's Hospital and Harvard Medical School, Harvard Institute of Medicine, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

ABSTRACT
Delta-catenin is a neuronal protein that contains 10 Armadillo motifs and binds to the juxtamembrane segment of classical cadherins. We report that delta-catenin interacts with cortactin in a tyrosine phosphorylation-dependent manner. This interaction occurs within a region of the delta-catenin sequence that is also essential for the neurite elongation effects. Src family kinases can phosphorylate delta-catenin and bind to delta-catenin through its polyproline tract. Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes. Conversely, increasing tyrosine phosphorylation disrupts the delta-catenin-cortactin complex. When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching. We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

Show MeSH