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Dual regulation of neuronal morphogenesis by a delta-catenin-cortactin complex and Rho.

Martinez MC, Ochiishi T, Majewski M, Kosik KS - J. Cell Biol. (2003)

Bottom Line: Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes.When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching.We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Neurology, Brigham and Women's Hospital and Harvard Medical School, Harvard Institute of Medicine, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

ABSTRACT
Delta-catenin is a neuronal protein that contains 10 Armadillo motifs and binds to the juxtamembrane segment of classical cadherins. We report that delta-catenin interacts with cortactin in a tyrosine phosphorylation-dependent manner. This interaction occurs within a region of the delta-catenin sequence that is also essential for the neurite elongation effects. Src family kinases can phosphorylate delta-catenin and bind to delta-catenin through its polyproline tract. Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes. Conversely, increasing tyrosine phosphorylation disrupts the delta-catenin-cortactin complex. When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching. We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

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δ-Catenin induces branching in PC12 cells. (A) GFP (a) and GFP-δ-catenin constructs (b) transfected in PC12 cells. Cells were fixed 36 h after transfection, and NGF was added at the time of the transfection. Bar, 5 μm. (B) PC12 cells (a) and δ-PC12 cells (b) were treated with NGF for 36 h. Cells were fixed and stained for actin. Quantification of the total process length and the number of primary and secondary processes is shown for PC12 and δ-PC12 cells after 36 h of NGF treatment. Bar, 5 μm.
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fig1: δ-Catenin induces branching in PC12 cells. (A) GFP (a) and GFP-δ-catenin constructs (b) transfected in PC12 cells. Cells were fixed 36 h after transfection, and NGF was added at the time of the transfection. Bar, 5 μm. (B) PC12 cells (a) and δ-PC12 cells (b) were treated with NGF for 36 h. Cells were fixed and stained for actin. Quantification of the total process length and the number of primary and secondary processes is shown for PC12 and δ-PC12 cells after 36 h of NGF treatment. Bar, 5 μm.

Mentions: A prominent effect of δ-catenin on cells is process elaboration. PC12 cells treated with NGF (100 ng/ml) at the time of GFP–δ-catenin transfection extended unbranched primary processes 36 h later (Fig. 1Figure 1.


Dual regulation of neuronal morphogenesis by a delta-catenin-cortactin complex and Rho.

Martinez MC, Ochiishi T, Majewski M, Kosik KS - J. Cell Biol. (2003)

δ-Catenin induces branching in PC12 cells. (A) GFP (a) and GFP-δ-catenin constructs (b) transfected in PC12 cells. Cells were fixed 36 h after transfection, and NGF was added at the time of the transfection. Bar, 5 μm. (B) PC12 cells (a) and δ-PC12 cells (b) were treated with NGF for 36 h. Cells were fixed and stained for actin. Quantification of the total process length and the number of primary and secondary processes is shown for PC12 and δ-PC12 cells after 36 h of NGF treatment. Bar, 5 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2172717&req=5

fig1: δ-Catenin induces branching in PC12 cells. (A) GFP (a) and GFP-δ-catenin constructs (b) transfected in PC12 cells. Cells were fixed 36 h after transfection, and NGF was added at the time of the transfection. Bar, 5 μm. (B) PC12 cells (a) and δ-PC12 cells (b) were treated with NGF for 36 h. Cells were fixed and stained for actin. Quantification of the total process length and the number of primary and secondary processes is shown for PC12 and δ-PC12 cells after 36 h of NGF treatment. Bar, 5 μm.
Mentions: A prominent effect of δ-catenin on cells is process elaboration. PC12 cells treated with NGF (100 ng/ml) at the time of GFP–δ-catenin transfection extended unbranched primary processes 36 h later (Fig. 1Figure 1.

Bottom Line: Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes.When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching.We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Neurology, Brigham and Women's Hospital and Harvard Medical School, Harvard Institute of Medicine, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

ABSTRACT
Delta-catenin is a neuronal protein that contains 10 Armadillo motifs and binds to the juxtamembrane segment of classical cadherins. We report that delta-catenin interacts with cortactin in a tyrosine phosphorylation-dependent manner. This interaction occurs within a region of the delta-catenin sequence that is also essential for the neurite elongation effects. Src family kinases can phosphorylate delta-catenin and bind to delta-catenin through its polyproline tract. Under conditions when tyrosine phosphorylation is reduced, delta-catenin binds to cortactin and cells extend unbranched primary processes. Conversely, increasing tyrosine phosphorylation disrupts the delta-catenin-cortactin complex. When RhoA is inhibited, delta-catenin enhances the effects of Rho inhibition on branching. We conclude that delta-catenin contributes to setting a balance between neurite elongation and branching in the elaboration of a complex dendritic tree.

Show MeSH