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A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product.

Rupp G, Porter ME - J. Cell Biol. (2003)

Bottom Line: The pf2-4 mutant displays an altered waveform that results in slow swimming cells.PF2 is a coiled-coil protein that shares significant homology with a mammalian growth arrest-specific gene product (Gas11/Gas8) and a trypanosome protein known as trypanin.The expression of Gas8/Gas11 transcripts in a wide range of tissues may also indicate a potential role for PF2-related proteins in other microtubule-based structures.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Cell Biology, and Development, University of Minnesota Medical School, 6-160 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA.

ABSTRACT
The dynein regulatory complex (DRC) is an important intermediate in the pathway that regulates flagellar motility. To identify subunits of the DRC, we characterized a Chlamydomonas motility mutant obtained by insertional mutagenesis. The pf2-4 mutant displays an altered waveform that results in slow swimming cells. EM analysis reveals defects in DRC structure that can be rescued by reintroduction of the wild-type PF2 gene. Immunolocalization studies show that the PF2 protein is distributed along the length of the axoneme, where it is part of a discrete complex of polypeptides. PF2 is a coiled-coil protein that shares significant homology with a mammalian growth arrest-specific gene product (Gas11/Gas8) and a trypanosome protein known as trypanin. PF2 and its homologues appear to be universal components of motile axonemes that are required for DRC assembly and the regulation of flagellar motility. The expression of Gas8/Gas11 transcripts in a wide range of tissues may also indicate a potential role for PF2-related proteins in other microtubule-based structures.

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PF2 homologues. (A) Clustal W alignment of the deduced amino acid sequence of the Chlamydomonas reinhardtii (Cr) PF2 gene (AY309087–AY309089) with homologues identified by BLAST. The shaded areas represent identical or conservatively substituted amino acids in >50% of the sequences. The underlined region represents a putative microtubule-binding domain identified in the trypanosome and human orthologues (Hill et al., 2000). The abbreviation and GenBank/EMBL/DDBJ accession nos. for the sequences are as follows: Danio rerio (Dr), AI584969; Drosophila melanogaster (Dm), AE003427 and AA440118; Homo sapiens (Hs), AF050079; Mus musculus (Mm), U19859; Trypanosoma bruzi (Tb), AAB81499; and Trypanosoma cruzi (Tc), AQ44061. Gas11, growth arrest–specific 11; Gas8, growth arrest–specific 8. (B) Phylogenetic analysis of the Cr PF2 sequence and its homologues.
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fig7: PF2 homologues. (A) Clustal W alignment of the deduced amino acid sequence of the Chlamydomonas reinhardtii (Cr) PF2 gene (AY309087–AY309089) with homologues identified by BLAST. The shaded areas represent identical or conservatively substituted amino acids in >50% of the sequences. The underlined region represents a putative microtubule-binding domain identified in the trypanosome and human orthologues (Hill et al., 2000). The abbreviation and GenBank/EMBL/DDBJ accession nos. for the sequences are as follows: Danio rerio (Dr), AI584969; Drosophila melanogaster (Dm), AE003427 and AA440118; Homo sapiens (Hs), AF050079; Mus musculus (Mm), U19859; Trypanosoma bruzi (Tb), AAB81499; and Trypanosoma cruzi (Tc), AQ44061. Gas11, growth arrest–specific 11; Gas8, growth arrest–specific 8. (B) Phylogenetic analysis of the Cr PF2 sequence and its homologues.

Mentions: The Chlamydomonas PF2 gene is predicted to encode a 471-aa polypeptide (Fig. 7Figure 7.


A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product.

Rupp G, Porter ME - J. Cell Biol. (2003)

PF2 homologues. (A) Clustal W alignment of the deduced amino acid sequence of the Chlamydomonas reinhardtii (Cr) PF2 gene (AY309087–AY309089) with homologues identified by BLAST. The shaded areas represent identical or conservatively substituted amino acids in >50% of the sequences. The underlined region represents a putative microtubule-binding domain identified in the trypanosome and human orthologues (Hill et al., 2000). The abbreviation and GenBank/EMBL/DDBJ accession nos. for the sequences are as follows: Danio rerio (Dr), AI584969; Drosophila melanogaster (Dm), AE003427 and AA440118; Homo sapiens (Hs), AF050079; Mus musculus (Mm), U19859; Trypanosoma bruzi (Tb), AAB81499; and Trypanosoma cruzi (Tc), AQ44061. Gas11, growth arrest–specific 11; Gas8, growth arrest–specific 8. (B) Phylogenetic analysis of the Cr PF2 sequence and its homologues.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2172716&req=5

fig7: PF2 homologues. (A) Clustal W alignment of the deduced amino acid sequence of the Chlamydomonas reinhardtii (Cr) PF2 gene (AY309087–AY309089) with homologues identified by BLAST. The shaded areas represent identical or conservatively substituted amino acids in >50% of the sequences. The underlined region represents a putative microtubule-binding domain identified in the trypanosome and human orthologues (Hill et al., 2000). The abbreviation and GenBank/EMBL/DDBJ accession nos. for the sequences are as follows: Danio rerio (Dr), AI584969; Drosophila melanogaster (Dm), AE003427 and AA440118; Homo sapiens (Hs), AF050079; Mus musculus (Mm), U19859; Trypanosoma bruzi (Tb), AAB81499; and Trypanosoma cruzi (Tc), AQ44061. Gas11, growth arrest–specific 11; Gas8, growth arrest–specific 8. (B) Phylogenetic analysis of the Cr PF2 sequence and its homologues.
Mentions: The Chlamydomonas PF2 gene is predicted to encode a 471-aa polypeptide (Fig. 7Figure 7.

Bottom Line: The pf2-4 mutant displays an altered waveform that results in slow swimming cells.PF2 is a coiled-coil protein that shares significant homology with a mammalian growth arrest-specific gene product (Gas11/Gas8) and a trypanosome protein known as trypanin.The expression of Gas8/Gas11 transcripts in a wide range of tissues may also indicate a potential role for PF2-related proteins in other microtubule-based structures.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Cell Biology, and Development, University of Minnesota Medical School, 6-160 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA.

ABSTRACT
The dynein regulatory complex (DRC) is an important intermediate in the pathway that regulates flagellar motility. To identify subunits of the DRC, we characterized a Chlamydomonas motility mutant obtained by insertional mutagenesis. The pf2-4 mutant displays an altered waveform that results in slow swimming cells. EM analysis reveals defects in DRC structure that can be rescued by reintroduction of the wild-type PF2 gene. Immunolocalization studies show that the PF2 protein is distributed along the length of the axoneme, where it is part of a discrete complex of polypeptides. PF2 is a coiled-coil protein that shares significant homology with a mammalian growth arrest-specific gene product (Gas11/Gas8) and a trypanosome protein known as trypanin. PF2 and its homologues appear to be universal components of motile axonemes that are required for DRC assembly and the regulation of flagellar motility. The expression of Gas8/Gas11 transcripts in a wide range of tissues may also indicate a potential role for PF2-related proteins in other microtubule-based structures.

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