Limits...
Lipid rafts make for slippery platforms.

Lai EC - J. Cell Biol. (2003)

Bottom Line: What's in a raft?Although cell membranes are certainly not homogeneous mixtures of lipids and proteins, almost all aspects of lipid rafts-how to define them, their size, composition, lifetime, and biological relevance-remain controversial.The answers will shape our views of signaling and of membrane dynamics.

View Article: PubMed Central - PubMed

Affiliation: lai@fruitfly.org

ABSTRACT
What's in a raft? Although cell membranes are certainly not homogeneous mixtures of lipids and proteins, almost all aspects of lipid rafts-how to define them, their size, composition, lifetime, and biological relevance-remain controversial. The answers will shape our views of signaling and of membrane dynamics.

Show MeSH
Lck near a site of activation (yellow) is more constrained than Lck farther away.Ike
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fig2: Lck near a site of activation (yellow) is more constrained than Lck farther away.Ike

Mentions: The new imaging techniques are now providing more direct visualization of raft involvement in these signaling events. During T cell activation, the T cell receptor (TCR) moves into rafts upon interaction with its cognate antigen and is phosphorylated by the Lck kinase. Kusumi and colleagues tracked single molecules of Lck-GFP by SFT and observed that it slows down following TCR activation, consistent with its association with an ordered raft domain (Ike et al., 2003) (Fig. 2). Kusumi sees similar dynamics by SPT for CD59, a complement-activated GPI-anchored protein that activates signaling to protect a cell against autoimmune attack. Following its clustering with antibody-coated particles to activate signal transduction, he says that “CD59 slows down eightfold following activation, and the clusters concentrate cholesterol,” which together strongly suggests raft formation (Subczynski and Kusumi, 2003).


Lipid rafts make for slippery platforms.

Lai EC - J. Cell Biol. (2003)

Lck near a site of activation (yellow) is more constrained than Lck farther away.Ike
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2172705&req=5

fig2: Lck near a site of activation (yellow) is more constrained than Lck farther away.Ike
Mentions: The new imaging techniques are now providing more direct visualization of raft involvement in these signaling events. During T cell activation, the T cell receptor (TCR) moves into rafts upon interaction with its cognate antigen and is phosphorylated by the Lck kinase. Kusumi and colleagues tracked single molecules of Lck-GFP by SFT and observed that it slows down following TCR activation, consistent with its association with an ordered raft domain (Ike et al., 2003) (Fig. 2). Kusumi sees similar dynamics by SPT for CD59, a complement-activated GPI-anchored protein that activates signaling to protect a cell against autoimmune attack. Following its clustering with antibody-coated particles to activate signal transduction, he says that “CD59 slows down eightfold following activation, and the clusters concentrate cholesterol,” which together strongly suggests raft formation (Subczynski and Kusumi, 2003).

Bottom Line: What's in a raft?Although cell membranes are certainly not homogeneous mixtures of lipids and proteins, almost all aspects of lipid rafts-how to define them, their size, composition, lifetime, and biological relevance-remain controversial.The answers will shape our views of signaling and of membrane dynamics.

View Article: PubMed Central - PubMed

Affiliation: lai@fruitfly.org

ABSTRACT
What's in a raft? Although cell membranes are certainly not homogeneous mixtures of lipids and proteins, almost all aspects of lipid rafts-how to define them, their size, composition, lifetime, and biological relevance-remain controversial. The answers will shape our views of signaling and of membrane dynamics.

Show MeSH