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A malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites.

Khater EI, Sinden RE, Dessens JT - J. Cell Biol. (2004)

Bottom Line: Knockout of PbIMC1a protein expression reduces, but does not abolish, sporozoite gliding locomotion.We identify a family of proteins related to PbIMC1a in Plasmodium and other apicomplexan parasites.These results provide new functional insight in the role of membrane skeletons in apicomplexan parasite biology.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Imperail College London, London SW7 2AZ, England, UK.

ABSTRACT
Membrane skeletons are structural elements that provide mechanical support to the plasma membrane and define cell shape. Here, we identify and characterize a putative protein component of the membrane skeleton of the malaria parasite. The protein, named PbIMC1a, is the structural orthologue of the Toxoplasma gondii inner membrane complex protein 1 (TgIMC1), a component of the membrane skeleton in tachyzoites. Using targeted gene disruption in the rodent malaria species Plasmodium berghei, we show that PbIMC1a is involved in sporozoite development, is necessary for providing normal sporozoite cell shape and mechanical stability, and is essential for sporozoite infectivity in insect and vertebrate hosts. Knockout of PbIMC1a protein expression reduces, but does not abolish, sporozoite gliding locomotion. We identify a family of proteins related to PbIMC1a in Plasmodium and other apicomplexan parasites. These results provide new functional insight in the role of membrane skeletons in apicomplexan parasite biology.

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Transcription and expression of PbIMC1a. (A) RT-PCR analysis of PbIMC1a and tubulin-1 mRNA in samples of asexual blood stages (AS), gametocytes (GC), ookinetes (OO), midguts with sporulating oocysts (SO), and sporozoite-infected salivary glands (SG). M: molecular weight markers. (B) IFA staining (FITC) of different life stages with anti-PbIMC1a antiserum. Phase: phase contrast. Blood stages are double-stained with DAPI (blue) to show merozoite and gametocyte nuclei.
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fig2: Transcription and expression of PbIMC1a. (A) RT-PCR analysis of PbIMC1a and tubulin-1 mRNA in samples of asexual blood stages (AS), gametocytes (GC), ookinetes (OO), midguts with sporulating oocysts (SO), and sporozoite-infected salivary glands (SG). M: molecular weight markers. (B) IFA staining (FITC) of different life stages with anti-PbIMC1a antiserum. Phase: phase contrast. Blood stages are double-stained with DAPI (blue) to show merozoite and gametocyte nuclei.

Mentions: We studied transcription of the PbIMC1a gene by RT-PCR analysis in different life stages of the parasite using cDNA-specific primers. PbIMC1a-specific mRNA was detected in samples of asexual blood stages, gametocytes, ookinetes, and sporulating oocysts. The highest amount of PbIMC1a-specific mRNA with respect to the reference gene tubulin-1 was present in sporulating oocysts, whereas PbIMC1a transcription was down-regulated in samples of sporozoite-infected mosquito salivary glands (Fig. 2 A).


A malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites.

Khater EI, Sinden RE, Dessens JT - J. Cell Biol. (2004)

Transcription and expression of PbIMC1a. (A) RT-PCR analysis of PbIMC1a and tubulin-1 mRNA in samples of asexual blood stages (AS), gametocytes (GC), ookinetes (OO), midguts with sporulating oocysts (SO), and sporozoite-infected salivary glands (SG). M: molecular weight markers. (B) IFA staining (FITC) of different life stages with anti-PbIMC1a antiserum. Phase: phase contrast. Blood stages are double-stained with DAPI (blue) to show merozoite and gametocyte nuclei.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2172497&req=5

fig2: Transcription and expression of PbIMC1a. (A) RT-PCR analysis of PbIMC1a and tubulin-1 mRNA in samples of asexual blood stages (AS), gametocytes (GC), ookinetes (OO), midguts with sporulating oocysts (SO), and sporozoite-infected salivary glands (SG). M: molecular weight markers. (B) IFA staining (FITC) of different life stages with anti-PbIMC1a antiserum. Phase: phase contrast. Blood stages are double-stained with DAPI (blue) to show merozoite and gametocyte nuclei.
Mentions: We studied transcription of the PbIMC1a gene by RT-PCR analysis in different life stages of the parasite using cDNA-specific primers. PbIMC1a-specific mRNA was detected in samples of asexual blood stages, gametocytes, ookinetes, and sporulating oocysts. The highest amount of PbIMC1a-specific mRNA with respect to the reference gene tubulin-1 was present in sporulating oocysts, whereas PbIMC1a transcription was down-regulated in samples of sporozoite-infected mosquito salivary glands (Fig. 2 A).

Bottom Line: Knockout of PbIMC1a protein expression reduces, but does not abolish, sporozoite gliding locomotion.We identify a family of proteins related to PbIMC1a in Plasmodium and other apicomplexan parasites.These results provide new functional insight in the role of membrane skeletons in apicomplexan parasite biology.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Imperail College London, London SW7 2AZ, England, UK.

ABSTRACT
Membrane skeletons are structural elements that provide mechanical support to the plasma membrane and define cell shape. Here, we identify and characterize a putative protein component of the membrane skeleton of the malaria parasite. The protein, named PbIMC1a, is the structural orthologue of the Toxoplasma gondii inner membrane complex protein 1 (TgIMC1), a component of the membrane skeleton in tachyzoites. Using targeted gene disruption in the rodent malaria species Plasmodium berghei, we show that PbIMC1a is involved in sporozoite development, is necessary for providing normal sporozoite cell shape and mechanical stability, and is essential for sporozoite infectivity in insect and vertebrate hosts. Knockout of PbIMC1a protein expression reduces, but does not abolish, sporozoite gliding locomotion. We identify a family of proteins related to PbIMC1a in Plasmodium and other apicomplexan parasites. These results provide new functional insight in the role of membrane skeletons in apicomplexan parasite biology.

Show MeSH
Related in: MedlinePlus