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Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut.

Fu M, Lui VC, Sham MH, Pachnis V, Tam PK - J. Cell Biol. (2004)

Bottom Line: The pro-neurogenic effect of glial cell line--derived neurotrophic factor (GDNF) on NCCs was abolished by Shh.In gut explants, NCCs migrated from the explants onto the adjacent substratum if GDNF was added, whereas addition of Shh abolished this migration.Our data suggest that Shh controls the proliferation and differentiation of NCCs and modulates the responsiveness of NCCs toward GDNF inductions.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, The University of Hong Kong, 21 Sassoon Rd., Pokfulam, Hong Kong, HKSAR China.

ABSTRACT
Enteric neural crest cells (NCCs) migrate and colonize the entire gut and proliferate and differentiate into neurons and glia of the enteric nervous system in vertebrate embryos. We have investigated the mitogenic and morphogenic functions of Sonic hedgehog (Shh) on enteric NCCs in cell and organ culture. Enteric NCCs expressed Shh receptor Patched and transcripts encoding the Shh signal transducer (Gli1). Shh promoted the proliferation and inhibited the differentiation of NCCs. The pro-neurogenic effect of glial cell line--derived neurotrophic factor (GDNF) on NCCs was abolished by Shh. In gut explants, NCCs migrated from the explants onto the adjacent substratum if GDNF was added, whereas addition of Shh abolished this migration. Neuronal differentiation and coalescence of neural crest--derived cells into myenteric plexuses in explants was repressed by the addition of Shh. Our data suggest that Shh controls the proliferation and differentiation of NCCs and modulates the responsiveness of NCCs toward GDNF inductions.

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Related in: MedlinePlus

Shh promotes the proliferation of NCCs in gut explants. Explants cultured in different treatments with BrdU for 3 d were mildly triturated and cytospun onto microscope glasses for staining with anti-Ret (A–D, red) and anti-BrdU (E–H, green) antibodies. (I–L) DAPI-stained nuclei of cell clumps in various treatments are shown. All photos were taken at the same magnification. Bar, 25 μm.
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fig8: Shh promotes the proliferation of NCCs in gut explants. Explants cultured in different treatments with BrdU for 3 d were mildly triturated and cytospun onto microscope glasses for staining with anti-Ret (A–D, red) and anti-BrdU (E–H, green) antibodies. (I–L) DAPI-stained nuclei of cell clumps in various treatments are shown. All photos were taken at the same magnification. Bar, 25 μm.

Mentions: Immunofluorescence was performed on cytospin preparations of mildly dissociated explants that were cultured in the presence of BrdU. Cell clumps were identified as clusters of Ret+ cells (Fig. 8, A–D), and proliferating cells were localized with anti-BrdU antibody (Fig. 8, E–H). Culturing explants for 3 d in different treatments did not affect the percentage of Ret+ cells in cell clumps (Table IV). However, treatments with Shh or Shh plus GDNF increased the percentage of Ret+/BrdU+ cells in cell clumps by nearly twice as much as those in control explants and explants treated with GDNF alone (Table IV).


Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut.

Fu M, Lui VC, Sham MH, Pachnis V, Tam PK - J. Cell Biol. (2004)

Shh promotes the proliferation of NCCs in gut explants. Explants cultured in different treatments with BrdU for 3 d were mildly triturated and cytospun onto microscope glasses for staining with anti-Ret (A–D, red) and anti-BrdU (E–H, green) antibodies. (I–L) DAPI-stained nuclei of cell clumps in various treatments are shown. All photos were taken at the same magnification. Bar, 25 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2172437&req=5

fig8: Shh promotes the proliferation of NCCs in gut explants. Explants cultured in different treatments with BrdU for 3 d were mildly triturated and cytospun onto microscope glasses for staining with anti-Ret (A–D, red) and anti-BrdU (E–H, green) antibodies. (I–L) DAPI-stained nuclei of cell clumps in various treatments are shown. All photos were taken at the same magnification. Bar, 25 μm.
Mentions: Immunofluorescence was performed on cytospin preparations of mildly dissociated explants that were cultured in the presence of BrdU. Cell clumps were identified as clusters of Ret+ cells (Fig. 8, A–D), and proliferating cells were localized with anti-BrdU antibody (Fig. 8, E–H). Culturing explants for 3 d in different treatments did not affect the percentage of Ret+ cells in cell clumps (Table IV). However, treatments with Shh or Shh plus GDNF increased the percentage of Ret+/BrdU+ cells in cell clumps by nearly twice as much as those in control explants and explants treated with GDNF alone (Table IV).

Bottom Line: The pro-neurogenic effect of glial cell line--derived neurotrophic factor (GDNF) on NCCs was abolished by Shh.In gut explants, NCCs migrated from the explants onto the adjacent substratum if GDNF was added, whereas addition of Shh abolished this migration.Our data suggest that Shh controls the proliferation and differentiation of NCCs and modulates the responsiveness of NCCs toward GDNF inductions.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, The University of Hong Kong, 21 Sassoon Rd., Pokfulam, Hong Kong, HKSAR China.

ABSTRACT
Enteric neural crest cells (NCCs) migrate and colonize the entire gut and proliferate and differentiate into neurons and glia of the enteric nervous system in vertebrate embryos. We have investigated the mitogenic and morphogenic functions of Sonic hedgehog (Shh) on enteric NCCs in cell and organ culture. Enteric NCCs expressed Shh receptor Patched and transcripts encoding the Shh signal transducer (Gli1). Shh promoted the proliferation and inhibited the differentiation of NCCs. The pro-neurogenic effect of glial cell line--derived neurotrophic factor (GDNF) on NCCs was abolished by Shh. In gut explants, NCCs migrated from the explants onto the adjacent substratum if GDNF was added, whereas addition of Shh abolished this migration. Neuronal differentiation and coalescence of neural crest--derived cells into myenteric plexuses in explants was repressed by the addition of Shh. Our data suggest that Shh controls the proliferation and differentiation of NCCs and modulates the responsiveness of NCCs toward GDNF inductions.

Show MeSH
Related in: MedlinePlus