Limits...
AHNAK interaction with the annexin 2/S100A10 complex regulates cell membrane cytoarchitecture.

Benaud C, Gentil BJ, Assard N, Court M, Garin J, Delphin C, Baudier J - J. Cell Biol. (2003)

Bottom Line: Down-regulation of both annexin 2 and S100A10 using an annexin 2-specific small interfering RNA inhibits the association of AHNAK with plasma membrane.In Madin-Darby canine kidney cells, down-regulation of AHNAK using AHNAK-specific small interfering RNA prevents cortical actin cytoskeleton reorganization required to support cell height.We propose that the interaction of AHNAK with the annexin 2/S100A10 regulates cortical actin cytoskeleton organization and cell membrane cytoarchitecture.

View Article: PubMed Central - PubMed

Affiliation: INSERM EMI-0104, DRDC-TS, CEA-Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.

ABSTRACT
Remodelling of the plasma membrane cytoarchitecture is crucial for the regulation of epithelial cell adhesion and permeability. In Madin-Darby canine kidney cells, the protein AHNAK relocates from the cytosol to the cytosolic surface of the plasma membrane during the formation of cell-cell contacts and the development of epithelial polarity. This targeting is reversible and regulated by Ca(2+)-dependent cell-cell adhesion. At the plasma membrane, AHNAK associates as a multimeric complex with actin and the annexin 2/S100A10 complex. The S100A10 subunit serves to mediate the interaction between annexin 2 and the COOH-terminal regulatory domain of AHNAK. Down-regulation of both annexin 2 and S100A10 using an annexin 2-specific small interfering RNA inhibits the association of AHNAK with plasma membrane. In Madin-Darby canine kidney cells, down-regulation of AHNAK using AHNAK-specific small interfering RNA prevents cortical actin cytoskeleton reorganization required to support cell height. We propose that the interaction of AHNAK with the annexin 2/S100A10 regulates cortical actin cytoskeleton organization and cell membrane cytoarchitecture.

Show MeSH

Related in: MedlinePlus

Effect of the invalidation of AHNAK and annexin 2 on cell height. Confocal images of MCF-7 cells transfected either with annexin 2 (A) or AHNAK (B) RNA interference. Apical (a and d), lateral (b and e), basal (c and f), and x-y sections and x-z merged sections (g and h) double immunostained for actin (A and B; d–f and h) and S100A10 (A; a–c and g) or AHNAK (B; a–c and g) reveals a similar collapse in cell height in both annexin 2– and AHNAK-invalidated cells. In A and B, x-y and x-z sections are from independent experiments.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2171952&req=5

fig6: Effect of the invalidation of AHNAK and annexin 2 on cell height. Confocal images of MCF-7 cells transfected either with annexin 2 (A) or AHNAK (B) RNA interference. Apical (a and d), lateral (b and e), basal (c and f), and x-y sections and x-z merged sections (g and h) double immunostained for actin (A and B; d–f and h) and S100A10 (A; a–c and g) or AHNAK (B; a–c and g) reveals a similar collapse in cell height in both annexin 2– and AHNAK-invalidated cells. In A and B, x-y and x-z sections are from independent experiments.

Mentions: Confocal microscopy analysis of actin distribution visualized with fluorescent phalloidin in control and annexin 2/S100A10-depleted cells confirmed the change in the morphology of cells with low annexin 2/S100A10 content (Fig. 6 A). The overall height of annexin 2/S100A10-depleted MCF-7 cells is decreased, with most of the actin-associated fluorescence found in the more basal focal planes. Similar changes in cell morphology and actin-associated fluorescence were also observed in MCF-7 cells depleted for AHNAK after transfection with AHNAK siRNA (Fig. 6 B; see following paragraph). Together, these data suggest that the membrane-associated annexin 2/S100A10 complex is essential to the recruitment of AHNAK at the plasma membrane, and that the annexin 2/S100A10/AHNAK complexes regulate actin-dependent cytoarchitecture.


AHNAK interaction with the annexin 2/S100A10 complex regulates cell membrane cytoarchitecture.

Benaud C, Gentil BJ, Assard N, Court M, Garin J, Delphin C, Baudier J - J. Cell Biol. (2003)

Effect of the invalidation of AHNAK and annexin 2 on cell height. Confocal images of MCF-7 cells transfected either with annexin 2 (A) or AHNAK (B) RNA interference. Apical (a and d), lateral (b and e), basal (c and f), and x-y sections and x-z merged sections (g and h) double immunostained for actin (A and B; d–f and h) and S100A10 (A; a–c and g) or AHNAK (B; a–c and g) reveals a similar collapse in cell height in both annexin 2– and AHNAK-invalidated cells. In A and B, x-y and x-z sections are from independent experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2171952&req=5

fig6: Effect of the invalidation of AHNAK and annexin 2 on cell height. Confocal images of MCF-7 cells transfected either with annexin 2 (A) or AHNAK (B) RNA interference. Apical (a and d), lateral (b and e), basal (c and f), and x-y sections and x-z merged sections (g and h) double immunostained for actin (A and B; d–f and h) and S100A10 (A; a–c and g) or AHNAK (B; a–c and g) reveals a similar collapse in cell height in both annexin 2– and AHNAK-invalidated cells. In A and B, x-y and x-z sections are from independent experiments.
Mentions: Confocal microscopy analysis of actin distribution visualized with fluorescent phalloidin in control and annexin 2/S100A10-depleted cells confirmed the change in the morphology of cells with low annexin 2/S100A10 content (Fig. 6 A). The overall height of annexin 2/S100A10-depleted MCF-7 cells is decreased, with most of the actin-associated fluorescence found in the more basal focal planes. Similar changes in cell morphology and actin-associated fluorescence were also observed in MCF-7 cells depleted for AHNAK after transfection with AHNAK siRNA (Fig. 6 B; see following paragraph). Together, these data suggest that the membrane-associated annexin 2/S100A10 complex is essential to the recruitment of AHNAK at the plasma membrane, and that the annexin 2/S100A10/AHNAK complexes regulate actin-dependent cytoarchitecture.

Bottom Line: Down-regulation of both annexin 2 and S100A10 using an annexin 2-specific small interfering RNA inhibits the association of AHNAK with plasma membrane.In Madin-Darby canine kidney cells, down-regulation of AHNAK using AHNAK-specific small interfering RNA prevents cortical actin cytoskeleton reorganization required to support cell height.We propose that the interaction of AHNAK with the annexin 2/S100A10 regulates cortical actin cytoskeleton organization and cell membrane cytoarchitecture.

View Article: PubMed Central - PubMed

Affiliation: INSERM EMI-0104, DRDC-TS, CEA-Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.

ABSTRACT
Remodelling of the plasma membrane cytoarchitecture is crucial for the regulation of epithelial cell adhesion and permeability. In Madin-Darby canine kidney cells, the protein AHNAK relocates from the cytosol to the cytosolic surface of the plasma membrane during the formation of cell-cell contacts and the development of epithelial polarity. This targeting is reversible and regulated by Ca(2+)-dependent cell-cell adhesion. At the plasma membrane, AHNAK associates as a multimeric complex with actin and the annexin 2/S100A10 complex. The S100A10 subunit serves to mediate the interaction between annexin 2 and the COOH-terminal regulatory domain of AHNAK. Down-regulation of both annexin 2 and S100A10 using an annexin 2-specific small interfering RNA inhibits the association of AHNAK with plasma membrane. In Madin-Darby canine kidney cells, down-regulation of AHNAK using AHNAK-specific small interfering RNA prevents cortical actin cytoskeleton reorganization required to support cell height. We propose that the interaction of AHNAK with the annexin 2/S100A10 regulates cortical actin cytoskeleton organization and cell membrane cytoarchitecture.

Show MeSH
Related in: MedlinePlus