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Retinal ganglion cell degeneration is topological but not cell type specific in DBA/2J mice.

Jakobs TC, Libby RT, Ben Y, John SW, Masland RH - J. Cell Biol. (2005)

Bottom Line: Regions of cell death or survival radiated from the optic nerve head in fan-shaped sectors.Collectively, the data suggest axon damage at the optic nerve head as an early lesion, and damage to axon bundles would cause this pattern of degeneration.However, the architecture of the mouse eye seems to preclude a commonly postulated source of mechanical damage within the nerve head.

View Article: PubMed Central - PubMed

Affiliation: Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02114.

ABSTRACT
Using a variety of double and triple labeling techniques, we have reevaluated the death of retinal neurons in a mouse model of hereditary glaucoma. Cell-specific markers and total neuron counts revealed no cell loss in any retinal neurons other than the ganglion cells. Within the limits of our ability to define cell types, no group of ganglion cells was especially vulnerable or resistant to degeneration. Retrograde labeling and neurofilament staining showed that axonal atrophy, dendritic remodeling, and somal shrinkage (at least of the largest cell types) precedes ganglion cell death in this glaucoma model. Regions of cell death or survival radiated from the optic nerve head in fan-shaped sectors. Collectively, the data suggest axon damage at the optic nerve head as an early lesion, and damage to axon bundles would cause this pattern of degeneration. However, the architecture of the mouse eye seems to preclude a commonly postulated source of mechanical damage within the nerve head.

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Sectors of fairly normal ganglion cells can persist even in severe retinas. (A) Survey of a whole-mounted retina stained with anti-SMI32 (green), Chat (red), and TOPRO (blue). The outlined square indicates the location of the field in B. (B) Higher magnification view. A sharp boundary between an axon- and cell-rich area (bottom) and a depleted area (left) is obvious. The optic nerve grade was severe for this eye. Bars, 500 μm.
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fig10: Sectors of fairly normal ganglion cells can persist even in severe retinas. (A) Survey of a whole-mounted retina stained with anti-SMI32 (green), Chat (red), and TOPRO (blue). The outlined square indicates the location of the field in B. (B) Higher magnification view. A sharp boundary between an axon- and cell-rich area (bottom) and a depleted area (left) is obvious. The optic nerve grade was severe for this eye. Bars, 500 μm.

Mentions: The sixth DBA/2J retina had major ganglion cell loss (nerve grade severe) but had a sector that remained unaffected (see Fig. 10). SMI32 cell diameters were measured for the affected and the normal part of this retina separately. Within the degenerated and normal zones the diameters were 15.8 ± 2.3 and 19.48 ± 2.62 μm, respectively (P < 0.001).


Retinal ganglion cell degeneration is topological but not cell type specific in DBA/2J mice.

Jakobs TC, Libby RT, Ben Y, John SW, Masland RH - J. Cell Biol. (2005)

Sectors of fairly normal ganglion cells can persist even in severe retinas. (A) Survey of a whole-mounted retina stained with anti-SMI32 (green), Chat (red), and TOPRO (blue). The outlined square indicates the location of the field in B. (B) Higher magnification view. A sharp boundary between an axon- and cell-rich area (bottom) and a depleted area (left) is obvious. The optic nerve grade was severe for this eye. Bars, 500 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2171185&req=5

fig10: Sectors of fairly normal ganglion cells can persist even in severe retinas. (A) Survey of a whole-mounted retina stained with anti-SMI32 (green), Chat (red), and TOPRO (blue). The outlined square indicates the location of the field in B. (B) Higher magnification view. A sharp boundary between an axon- and cell-rich area (bottom) and a depleted area (left) is obvious. The optic nerve grade was severe for this eye. Bars, 500 μm.
Mentions: The sixth DBA/2J retina had major ganglion cell loss (nerve grade severe) but had a sector that remained unaffected (see Fig. 10). SMI32 cell diameters were measured for the affected and the normal part of this retina separately. Within the degenerated and normal zones the diameters were 15.8 ± 2.3 and 19.48 ± 2.62 μm, respectively (P < 0.001).

Bottom Line: Regions of cell death or survival radiated from the optic nerve head in fan-shaped sectors.Collectively, the data suggest axon damage at the optic nerve head as an early lesion, and damage to axon bundles would cause this pattern of degeneration.However, the architecture of the mouse eye seems to preclude a commonly postulated source of mechanical damage within the nerve head.

View Article: PubMed Central - PubMed

Affiliation: Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02114.

ABSTRACT
Using a variety of double and triple labeling techniques, we have reevaluated the death of retinal neurons in a mouse model of hereditary glaucoma. Cell-specific markers and total neuron counts revealed no cell loss in any retinal neurons other than the ganglion cells. Within the limits of our ability to define cell types, no group of ganglion cells was especially vulnerable or resistant to degeneration. Retrograde labeling and neurofilament staining showed that axonal atrophy, dendritic remodeling, and somal shrinkage (at least of the largest cell types) precedes ganglion cell death in this glaucoma model. Regions of cell death or survival radiated from the optic nerve head in fan-shaped sectors. Collectively, the data suggest axon damage at the optic nerve head as an early lesion, and damage to axon bundles would cause this pattern of degeneration. However, the architecture of the mouse eye seems to preclude a commonly postulated source of mechanical damage within the nerve head.

Show MeSH
Related in: MedlinePlus