Limits...
Recombination protein Tid1p controls resolution of cohesin-dependent linkages in meiosis in Saccharomyces cerevisiae.

Kateneva AV, Konovchenko AA, Guacci V, Dresser ME - J. Cell Biol. (2005)

Bottom Line: Genetic results indicate that the primary defect in these cells is a failure to resolve Mcd1p-mediated connections.Tid1p interacts with recombination enzymes Dmc1p and Rad51p and has an established role in recombination repair.We propose that Tid1p remodels Mcd1p-mediated cohesion early in meiotic prophase to facilitate interhomologue recombination and the subsequent segregation of homologous chromosomes.

View Article: PubMed Central - PubMed

Affiliation: Program in Molecular, Cell, and Developmental Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104.

ABSTRACT
Sister chromatid cohesion and interhomologue recombination are coordinated to promote the segregation of homologous chromosomes instead of sister chromatids at the first meiotic division. During meiotic prophase in Saccharomyces cerevisiae, the meiosis-specific cohesin Rec8p localizes along chromosome axes and mediates most of the cohesion. The mitotic cohesin Mcd1p/Scc1p localizes to discrete spots along chromosome arms, and its function is not clear. In cells lacking Tid1p, which is a member of the SWI2/SNF2 family of helicase-like proteins that are involved in chromatin remodeling, Mcd1p and Rec8p persist abnormally through both meiotic divisions, and chromosome segregation fails in the majority of cells. Genetic results indicate that the primary defect in these cells is a failure to resolve Mcd1p-mediated connections. Tid1p interacts with recombination enzymes Dmc1p and Rad51p and has an established role in recombination repair. We propose that Tid1p remodels Mcd1p-mediated cohesion early in meiotic prophase to facilitate interhomologue recombination and the subsequent segregation of homologous chromosomes.

Show MeSH

Related in: MedlinePlus

Suppression of tid1Δ by the deletion of SPO11. Progression of cells through the divisions was monitored by staining chromatin with DAPI. (A) Segregation of chromosomes in spo11Δ. Gray lines represent chromatids. Thickenings on gray lines are centromeres. Black dots are cohesins. Arrows represent the pulling forces of spindles. (B) Kinetics of entry into anaphase I. Cells at or past anaphase I are anaphase I and II dumbbells, binucleates, and tetranucleates. (C) Anaphase I dumbbells. At least 200 cells were counted at each time point.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2171180&req=5

fig2: Suppression of tid1Δ by the deletion of SPO11. Progression of cells through the divisions was monitored by staining chromatin with DAPI. (A) Segregation of chromosomes in spo11Δ. Gray lines represent chromatids. Thickenings on gray lines are centromeres. Black dots are cohesins. Arrows represent the pulling forces of spindles. (B) Kinetics of entry into anaphase I. Cells at or past anaphase I are anaphase I and II dumbbells, binucleates, and tetranucleates. (C) Anaphase I dumbbells. At least 200 cells were counted at each time point.

Mentions: To prove that pachytene arrest of the majority of tid1Δ cells depends on DSB formation, the SPO11 gene, which encodes the meiosis-specific endonuclease that is responsible for producing DSBs and chiasmata (Cao et al., 1990; Keeney et al., 1997), was deleted, and the progression of cells through meiotic divisions was monitored by staining chromatin with DAPI. spo11Δ suppresses the pachytene arrest of tid1Δ, allowing 68% of spo11Δ tid1Δ cells to enter the divisions without delay (Fig. 2 B), which supports the idea that the tid1Δ pachytene arrest is caused by unrepaired DSBs.


Recombination protein Tid1p controls resolution of cohesin-dependent linkages in meiosis in Saccharomyces cerevisiae.

Kateneva AV, Konovchenko AA, Guacci V, Dresser ME - J. Cell Biol. (2005)

Suppression of tid1Δ by the deletion of SPO11. Progression of cells through the divisions was monitored by staining chromatin with DAPI. (A) Segregation of chromosomes in spo11Δ. Gray lines represent chromatids. Thickenings on gray lines are centromeres. Black dots are cohesins. Arrows represent the pulling forces of spindles. (B) Kinetics of entry into anaphase I. Cells at or past anaphase I are anaphase I and II dumbbells, binucleates, and tetranucleates. (C) Anaphase I dumbbells. At least 200 cells were counted at each time point.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2171180&req=5

fig2: Suppression of tid1Δ by the deletion of SPO11. Progression of cells through the divisions was monitored by staining chromatin with DAPI. (A) Segregation of chromosomes in spo11Δ. Gray lines represent chromatids. Thickenings on gray lines are centromeres. Black dots are cohesins. Arrows represent the pulling forces of spindles. (B) Kinetics of entry into anaphase I. Cells at or past anaphase I are anaphase I and II dumbbells, binucleates, and tetranucleates. (C) Anaphase I dumbbells. At least 200 cells were counted at each time point.
Mentions: To prove that pachytene arrest of the majority of tid1Δ cells depends on DSB formation, the SPO11 gene, which encodes the meiosis-specific endonuclease that is responsible for producing DSBs and chiasmata (Cao et al., 1990; Keeney et al., 1997), was deleted, and the progression of cells through meiotic divisions was monitored by staining chromatin with DAPI. spo11Δ suppresses the pachytene arrest of tid1Δ, allowing 68% of spo11Δ tid1Δ cells to enter the divisions without delay (Fig. 2 B), which supports the idea that the tid1Δ pachytene arrest is caused by unrepaired DSBs.

Bottom Line: Genetic results indicate that the primary defect in these cells is a failure to resolve Mcd1p-mediated connections.Tid1p interacts with recombination enzymes Dmc1p and Rad51p and has an established role in recombination repair.We propose that Tid1p remodels Mcd1p-mediated cohesion early in meiotic prophase to facilitate interhomologue recombination and the subsequent segregation of homologous chromosomes.

View Article: PubMed Central - PubMed

Affiliation: Program in Molecular, Cell, and Developmental Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104.

ABSTRACT
Sister chromatid cohesion and interhomologue recombination are coordinated to promote the segregation of homologous chromosomes instead of sister chromatids at the first meiotic division. During meiotic prophase in Saccharomyces cerevisiae, the meiosis-specific cohesin Rec8p localizes along chromosome axes and mediates most of the cohesion. The mitotic cohesin Mcd1p/Scc1p localizes to discrete spots along chromosome arms, and its function is not clear. In cells lacking Tid1p, which is a member of the SWI2/SNF2 family of helicase-like proteins that are involved in chromatin remodeling, Mcd1p and Rec8p persist abnormally through both meiotic divisions, and chromosome segregation fails in the majority of cells. Genetic results indicate that the primary defect in these cells is a failure to resolve Mcd1p-mediated connections. Tid1p interacts with recombination enzymes Dmc1p and Rad51p and has an established role in recombination repair. We propose that Tid1p remodels Mcd1p-mediated cohesion early in meiotic prophase to facilitate interhomologue recombination and the subsequent segregation of homologous chromosomes.

Show MeSH
Related in: MedlinePlus