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Antioxidant activities of uyaku (lindera strychnifolia) leaf extract: a natural extract used in traditional medicine.

Noda Y, Mori A - J Clin Biochem Nutr (2007)

Bottom Line: Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine.In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation.These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

View Article: PubMed Central - PubMed

Affiliation: National Heat, Lung, and Blood Institute, National Institute of Health, Bethesda, MD 20892-8012, U.S.A.

ABSTRACT
Uyaku (Lindera strychnifolia, Sieb. et Zucc.) is used in traditional Asian medicine to treat stomach and renal diseases, neuralgia, rheumatism, and aging. In this study, the effects of lyophilized extracts on hydroxyl ((.)OH) and superoxide (O(2) (.-)) radicals were examined using an electron spin resonance (ESR) spectrometer with the spin trap, 5,5'-dimethyl-1-pyrroline-N-oxide. Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine. Analysis of ESR data of the extracts indicated the direct (.)OH and O(2) (.-) scavenging. The extracts scavenged NO(.) in a dose-dependent manner, inhibited lipid peroxidation of linolenic acid, and protein carbonyl formation in bovine serum albumin. In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation. These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effect of Uyaku leaf hot-water extract on protein carbonyl formation in bovine serum albumin. The data was obtained from three separate experiments.
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Figure 5: Inhibitory effect of Uyaku leaf hot-water extract on protein carbonyl formation in bovine serum albumin. The data was obtained from three separate experiments.

Mentions: The hot-water extracts slightly inhibited protein carbonyl formation in BSA in a dose-dependent manner (Fig. 5). Therefore, the precise ID50 value could not be obtained in this assay. At a dose of 0.12 mg of hot-water extract/ml during the oxidation using (Fe2+ + H2O2) system, the percentage value of inhibition was 30% when compared to the control (without Uyaku). The protein carbonyl formation in BSA (control: without Uyaku extract) was approximately 3.6 n moles of carbonyl groups/mg of protein. The sample solution was slightly yellow; therefore, the values of absorbance were corrected by subtracting the control values (without 2,4-dinitrophenylhydrazine for each concentration of the sample).


Antioxidant activities of uyaku (lindera strychnifolia) leaf extract: a natural extract used in traditional medicine.

Noda Y, Mori A - J Clin Biochem Nutr (2007)

Inhibitory effect of Uyaku leaf hot-water extract on protein carbonyl formation in bovine serum albumin. The data was obtained from three separate experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2170949&req=5

Figure 5: Inhibitory effect of Uyaku leaf hot-water extract on protein carbonyl formation in bovine serum albumin. The data was obtained from three separate experiments.
Mentions: The hot-water extracts slightly inhibited protein carbonyl formation in BSA in a dose-dependent manner (Fig. 5). Therefore, the precise ID50 value could not be obtained in this assay. At a dose of 0.12 mg of hot-water extract/ml during the oxidation using (Fe2+ + H2O2) system, the percentage value of inhibition was 30% when compared to the control (without Uyaku). The protein carbonyl formation in BSA (control: without Uyaku extract) was approximately 3.6 n moles of carbonyl groups/mg of protein. The sample solution was slightly yellow; therefore, the values of absorbance were corrected by subtracting the control values (without 2,4-dinitrophenylhydrazine for each concentration of the sample).

Bottom Line: Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine.In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation.These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

View Article: PubMed Central - PubMed

Affiliation: National Heat, Lung, and Blood Institute, National Institute of Health, Bethesda, MD 20892-8012, U.S.A.

ABSTRACT
Uyaku (Lindera strychnifolia, Sieb. et Zucc.) is used in traditional Asian medicine to treat stomach and renal diseases, neuralgia, rheumatism, and aging. In this study, the effects of lyophilized extracts on hydroxyl ((.)OH) and superoxide (O(2) (.-)) radicals were examined using an electron spin resonance (ESR) spectrometer with the spin trap, 5,5'-dimethyl-1-pyrroline-N-oxide. Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine. Analysis of ESR data of the extracts indicated the direct (.)OH and O(2) (.-) scavenging. The extracts scavenged NO(.) in a dose-dependent manner, inhibited lipid peroxidation of linolenic acid, and protein carbonyl formation in bovine serum albumin. In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation. These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

No MeSH data available.


Related in: MedlinePlus