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Antioxidant activities of uyaku (lindera strychnifolia) leaf extract: a natural extract used in traditional medicine.

Noda Y, Mori A - J Clin Biochem Nutr (2007)

Bottom Line: Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine.In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation.These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

View Article: PubMed Central - PubMed

Affiliation: National Heat, Lung, and Blood Institute, National Institute of Health, Bethesda, MD 20892-8012, U.S.A.

ABSTRACT
Uyaku (Lindera strychnifolia, Sieb. et Zucc.) is used in traditional Asian medicine to treat stomach and renal diseases, neuralgia, rheumatism, and aging. In this study, the effects of lyophilized extracts on hydroxyl ((.)OH) and superoxide (O(2) (.-)) radicals were examined using an electron spin resonance (ESR) spectrometer with the spin trap, 5,5'-dimethyl-1-pyrroline-N-oxide. Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine. Analysis of ESR data of the extracts indicated the direct (.)OH and O(2) (.-) scavenging. The extracts scavenged NO(.) in a dose-dependent manner, inhibited lipid peroxidation of linolenic acid, and protein carbonyl formation in bovine serum albumin. In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation. These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effect of Uyaku leaf hot-water extract on NO generation. The data points represent 30 min after addition of NOC-7 (20 µM). The reproducibility was confirmed by two separate experiments.
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Figure 3: Inhibitory effect of Uyaku leaf hot-water extract on NO generation. The data points represent 30 min after addition of NOC-7 (20 µM). The reproducibility was confirmed by two separate experiments.

Mentions: The hot-water extract showed a dose-dependent NO· scavenging activity (Fig. 3). The sample solution itself was slightly yellow at higher concentrations (up to 10 mg/ml), and color varied in a dose-dependent manner; therefore, the observed absorbance was corrected by subtracting the control values (for each concentration of the sample). The ID50 values were approximately 2 to 3 mg/ml.


Antioxidant activities of uyaku (lindera strychnifolia) leaf extract: a natural extract used in traditional medicine.

Noda Y, Mori A - J Clin Biochem Nutr (2007)

Inhibitory effect of Uyaku leaf hot-water extract on NO generation. The data points represent 30 min after addition of NOC-7 (20 µM). The reproducibility was confirmed by two separate experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2170949&req=5

Figure 3: Inhibitory effect of Uyaku leaf hot-water extract on NO generation. The data points represent 30 min after addition of NOC-7 (20 µM). The reproducibility was confirmed by two separate experiments.
Mentions: The hot-water extract showed a dose-dependent NO· scavenging activity (Fig. 3). The sample solution itself was slightly yellow at higher concentrations (up to 10 mg/ml), and color varied in a dose-dependent manner; therefore, the observed absorbance was corrected by subtracting the control values (for each concentration of the sample). The ID50 values were approximately 2 to 3 mg/ml.

Bottom Line: Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine.In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation.These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

View Article: PubMed Central - PubMed

Affiliation: National Heat, Lung, and Blood Institute, National Institute of Health, Bethesda, MD 20892-8012, U.S.A.

ABSTRACT
Uyaku (Lindera strychnifolia, Sieb. et Zucc.) is used in traditional Asian medicine to treat stomach and renal diseases, neuralgia, rheumatism, and aging. In this study, the effects of lyophilized extracts on hydroxyl ((.)OH) and superoxide (O(2) (.-)) radicals were examined using an electron spin resonance (ESR) spectrometer with the spin trap, 5,5'-dimethyl-1-pyrroline-N-oxide. Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine. Analysis of ESR data of the extracts indicated the direct (.)OH and O(2) (.-) scavenging. The extracts scavenged NO(.) in a dose-dependent manner, inhibited lipid peroxidation of linolenic acid, and protein carbonyl formation in bovine serum albumin. In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation. These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

No MeSH data available.


Related in: MedlinePlus