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Antioxidant activities of uyaku (lindera strychnifolia) leaf extract: a natural extract used in traditional medicine.

Noda Y, Mori A - J Clin Biochem Nutr (2007)

Bottom Line: Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine.In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation.These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

View Article: PubMed Central - PubMed

Affiliation: National Heat, Lung, and Blood Institute, National Institute of Health, Bethesda, MD 20892-8012, U.S.A.

ABSTRACT
Uyaku (Lindera strychnifolia, Sieb. et Zucc.) is used in traditional Asian medicine to treat stomach and renal diseases, neuralgia, rheumatism, and aging. In this study, the effects of lyophilized extracts on hydroxyl ((.)OH) and superoxide (O(2) (.-)) radicals were examined using an electron spin resonance (ESR) spectrometer with the spin trap, 5,5'-dimethyl-1-pyrroline-N-oxide. Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine. Analysis of ESR data of the extracts indicated the direct (.)OH and O(2) (.-) scavenging. The extracts scavenged NO(.) in a dose-dependent manner, inhibited lipid peroxidation of linolenic acid, and protein carbonyl formation in bovine serum albumin. In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation. These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

No MeSH data available.


Related in: MedlinePlus

a) Typical ESR spectra of DMPO-OOH at the various concentration of Uyaku leaf extract. The hyperfine coupling constants of spin adducts, DMPO-OOH were αN = 1.42 mT, aΗβ = 1.15 mT, and aHγ = 0.13 mT. b) A dose-dependent curve of SOD-like activity for Uyaku leaf hot-water extract. The data were obtained by two separate experiments. The symbols of the open diamond and the closed diamond represent the data points obtained by using 450 mM and 45 mM DMPO (each final concentration), respectively.
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Figure 2: a) Typical ESR spectra of DMPO-OOH at the various concentration of Uyaku leaf extract. The hyperfine coupling constants of spin adducts, DMPO-OOH were αN = 1.42 mT, aΗβ = 1.15 mT, and aHγ = 0.13 mT. b) A dose-dependent curve of SOD-like activity for Uyaku leaf hot-water extract. The data were obtained by two separate experiments. The symbols of the open diamond and the closed diamond represent the data points obtained by using 450 mM and 45 mM DMPO (each final concentration), respectively.

Mentions: The hot-water extract scavenged O2·− in a dose-dependent manner. The ID50 value of hot-water decreased 10-fold when a 10-fold lower concentration of DMPO was used. The typical ESR spectra and dose-response curve for the hot-water extract are shown in Fig. 2a and b, respectively. The ID50 value of hot-water extract was 0.01 mg/ml when 450 mM DMPO (final concentration) was used.


Antioxidant activities of uyaku (lindera strychnifolia) leaf extract: a natural extract used in traditional medicine.

Noda Y, Mori A - J Clin Biochem Nutr (2007)

a) Typical ESR spectra of DMPO-OOH at the various concentration of Uyaku leaf extract. The hyperfine coupling constants of spin adducts, DMPO-OOH were αN = 1.42 mT, aΗβ = 1.15 mT, and aHγ = 0.13 mT. b) A dose-dependent curve of SOD-like activity for Uyaku leaf hot-water extract. The data were obtained by two separate experiments. The symbols of the open diamond and the closed diamond represent the data points obtained by using 450 mM and 45 mM DMPO (each final concentration), respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2170949&req=5

Figure 2: a) Typical ESR spectra of DMPO-OOH at the various concentration of Uyaku leaf extract. The hyperfine coupling constants of spin adducts, DMPO-OOH were αN = 1.42 mT, aΗβ = 1.15 mT, and aHγ = 0.13 mT. b) A dose-dependent curve of SOD-like activity for Uyaku leaf hot-water extract. The data were obtained by two separate experiments. The symbols of the open diamond and the closed diamond represent the data points obtained by using 450 mM and 45 mM DMPO (each final concentration), respectively.
Mentions: The hot-water extract scavenged O2·− in a dose-dependent manner. The ID50 value of hot-water decreased 10-fold when a 10-fold lower concentration of DMPO was used. The typical ESR spectra and dose-response curve for the hot-water extract are shown in Fig. 2a and b, respectively. The ID50 value of hot-water extract was 0.01 mg/ml when 450 mM DMPO (final concentration) was used.

Bottom Line: Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine.In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation.These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

View Article: PubMed Central - PubMed

Affiliation: National Heat, Lung, and Blood Institute, National Institute of Health, Bethesda, MD 20892-8012, U.S.A.

ABSTRACT
Uyaku (Lindera strychnifolia, Sieb. et Zucc.) is used in traditional Asian medicine to treat stomach and renal diseases, neuralgia, rheumatism, and aging. In this study, the effects of lyophilized extracts on hydroxyl ((.)OH) and superoxide (O(2) (.-)) radicals were examined using an electron spin resonance (ESR) spectrometer with the spin trap, 5,5'-dimethyl-1-pyrroline-N-oxide. Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine. Analysis of ESR data of the extracts indicated the direct (.)OH and O(2) (.-) scavenging. The extracts scavenged NO(.) in a dose-dependent manner, inhibited lipid peroxidation of linolenic acid, and protein carbonyl formation in bovine serum albumin. In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation. These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

No MeSH data available.


Related in: MedlinePlus