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Antioxidant activities of uyaku (lindera strychnifolia) leaf extract: a natural extract used in traditional medicine.

Noda Y, Mori A - J Clin Biochem Nutr (2007)

Bottom Line: Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine.In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation.These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

View Article: PubMed Central - PubMed

Affiliation: National Heat, Lung, and Blood Institute, National Institute of Health, Bethesda, MD 20892-8012, U.S.A.

ABSTRACT
Uyaku (Lindera strychnifolia, Sieb. et Zucc.) is used in traditional Asian medicine to treat stomach and renal diseases, neuralgia, rheumatism, and aging. In this study, the effects of lyophilized extracts on hydroxyl ((.)OH) and superoxide (O(2) (.-)) radicals were examined using an electron spin resonance (ESR) spectrometer with the spin trap, 5,5'-dimethyl-1-pyrroline-N-oxide. Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine. Analysis of ESR data of the extracts indicated the direct (.)OH and O(2) (.-) scavenging. The extracts scavenged NO(.) in a dose-dependent manner, inhibited lipid peroxidation of linolenic acid, and protein carbonyl formation in bovine serum albumin. In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation. These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

No MeSH data available.


Related in: MedlinePlus

Representative dose-response curve of hydroxyl radical scavenging activity for Uyaku leaf hot-water extract. The data were obtained by two separate experiments. The symbols of the opened diamond and the closed diamond represent the data points obtained by using 4.5 mM and 0.45 mM DMPO (each final concentration), respectively.
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Figure 1: Representative dose-response curve of hydroxyl radical scavenging activity for Uyaku leaf hot-water extract. The data were obtained by two separate experiments. The symbols of the opened diamond and the closed diamond represent the data points obtained by using 4.5 mM and 0.45 mM DMPO (each final concentration), respectively.

Mentions: The leaf extract showed dose-dependent ·OH scavenging activity. A representative dose-response curve for hot-water extract is shown in Fig. 1. To examine the radical scavenging activity, 4.5 mM DMPO (final concentration) and a 10-fold lower concentration of DMPO (0.45 mM, final concentration) were used. The ID50 value of hot-water extract decreased 10-fold when a 10-fold lower concentration of DMPO was used. The ID50 value was 1.0 mg/ml when 4.5 mM DMPO (final concentration) was used. In comparison, under the same experimental condition, the ID50 value of Trolox (water-soluble vitamin E) was 0.3 mg/ml (1.2 mM) and the ID50 value of ascorbate was 2 µg/ml (11 µM).


Antioxidant activities of uyaku (lindera strychnifolia) leaf extract: a natural extract used in traditional medicine.

Noda Y, Mori A - J Clin Biochem Nutr (2007)

Representative dose-response curve of hydroxyl radical scavenging activity for Uyaku leaf hot-water extract. The data were obtained by two separate experiments. The symbols of the opened diamond and the closed diamond represent the data points obtained by using 4.5 mM and 0.45 mM DMPO (each final concentration), respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2170949&req=5

Figure 1: Representative dose-response curve of hydroxyl radical scavenging activity for Uyaku leaf hot-water extract. The data were obtained by two separate experiments. The symbols of the opened diamond and the closed diamond represent the data points obtained by using 4.5 mM and 0.45 mM DMPO (each final concentration), respectively.
Mentions: The leaf extract showed dose-dependent ·OH scavenging activity. A representative dose-response curve for hot-water extract is shown in Fig. 1. To examine the radical scavenging activity, 4.5 mM DMPO (final concentration) and a 10-fold lower concentration of DMPO (0.45 mM, final concentration) were used. The ID50 value of hot-water extract decreased 10-fold when a 10-fold lower concentration of DMPO was used. The ID50 value was 1.0 mg/ml when 4.5 mM DMPO (final concentration) was used. In comparison, under the same experimental condition, the ID50 value of Trolox (water-soluble vitamin E) was 0.3 mg/ml (1.2 mM) and the ID50 value of ascorbate was 2 µg/ml (11 µM).

Bottom Line: Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine.In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation.These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

View Article: PubMed Central - PubMed

Affiliation: National Heat, Lung, and Blood Institute, National Institute of Health, Bethesda, MD 20892-8012, U.S.A.

ABSTRACT
Uyaku (Lindera strychnifolia, Sieb. et Zucc.) is used in traditional Asian medicine to treat stomach and renal diseases, neuralgia, rheumatism, and aging. In this study, the effects of lyophilized extracts on hydroxyl ((.)OH) and superoxide (O(2) (.-)) radicals were examined using an electron spin resonance (ESR) spectrometer with the spin trap, 5,5'-dimethyl-1-pyrroline-N-oxide. Inhibitory effects were assessed using the following reagents: for nitric oxide (NO(.)), the Griess reagent; for (Fe(2+) + H(2)O(2))-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe(2+) + H(2)O(2))-induced protein carbonyl, 2,4-dinitrophenylhydrazine. Analysis of ESR data of the extracts indicated the direct (.)OH and O(2) (.-) scavenging. The extracts scavenged NO(.) in a dose-dependent manner, inhibited lipid peroxidation of linolenic acid, and protein carbonyl formation in bovine serum albumin. In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation. These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.

No MeSH data available.


Related in: MedlinePlus