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Using single-cell analysis, Waterhouse et al. (page 319) tracked changes in the mitochondrial transmembrane potential after apoptotic cytochrome c release and found that, in the absence of caspase activity, permeabilized mitochondria can regenerate their membrane potential sufficiently to generate ATP... In the new work, cells were triggered to undergo apoptosis in the presence of caspase inhibitors, causing the release of cytochrome c from mitochondria but preventing downstream apoptotic events... In this system, the mitochondrial transmembrane potential rapidly depolarizes, but recovers to its original levels within 60 min, after which it is maintained at levels sufficient to generate ATP using cytoplasmic cytochrome c... The findings conflict with observations from bulk-cell analysis, which have not shown a transient loss of the mitochondrial transmembrane potential... However, since mitochondrial membrane permeabilization is not synchronized in a population of cells, the loss and rapid recovery of membrane potential in individual cells would appear as an overall maintenance of membrane potential in the population... In this model, an off-center nucleus would be pushed by microtubules more frequently and more forcefully from the side closest to a cell tip, driving the nucleus back to the center... A computer model of this process shows that this mechanism can largely account for observed nuclear positioning movements.

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View Article: PubMed Central

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Using single-cell analysis, Waterhouse et al. (page 319) tracked changes in the mitochondrial transmembrane potential after apoptotic cytochrome c release and found that, in the absence of caspase activity, permeabilized mitochondria can regenerate their membrane potential sufficiently to generate ATP... In the new work, cells were triggered to undergo apoptosis in the presence of caspase inhibitors, causing the release of cytochrome c from mitochondria but preventing downstream apoptotic events... In this system, the mitochondrial transmembrane potential rapidly depolarizes, but recovers to its original levels within 60 min, after which it is maintained at levels sufficient to generate ATP using cytoplasmic cytochrome c... The findings conflict with observations from bulk-cell analysis, which have not shown a transient loss of the mitochondrial transmembrane potential... However, since mitochondrial membrane permeabilization is not synchronized in a population of cells, the loss and rapid recovery of membrane potential in individual cells would appear as an overall maintenance of membrane potential in the population... In this model, an off-center nucleus would be pushed by microtubules more frequently and more forcefully from the side closest to a cell tip, driving the nucleus back to the center... A computer model of this process shows that this mechanism can largely account for observed nuclear positioning movements.

No MeSH data available.