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Visualization of a cytoskeleton-like FtsZ network in chloroplasts.

Kiessling J, Kruse S, Rensing SA, Harter K, Decker EL, Reski R - J. Cell Biol. (2000)

Bottom Line: It has been a long-standing dogma in life sciences that only eukaryotic organisms possess a cytoskeleton.Here, we report two nuclear-encoded plant ftsZ genes which are highly conserved in coding sequence and intron structure.As these networks resemble the eukaryotic cytoskeleton in form and function, we suggest the term "plastoskeleton" for this newly described subcellular structure.

View Article: PubMed Central - PubMed

Affiliation: University of Freiburg, Plant Biotechnology, D-79104 Freiburg, Germany.

ABSTRACT
It has been a long-standing dogma in life sciences that only eukaryotic organisms possess a cytoskeleton. Recently, this belief was questioned by the finding that the bacterial cell division protein FtsZ resembles tubulin in sequence and structure and, thus, may be the progenitor of this major eukaryotic cytoskeletal element. Here, we report two nuclear-encoded plant ftsZ genes which are highly conserved in coding sequence and intron structure. Both their encoded proteins are imported into plastids and there, like in bacteria, they act on the division process in a dose-dependent manner. Whereas in bacteria FtsZ only transiently polymerizes to a ring-like structure, in chloroplasts we identified persistent, highly organized filamentous scaffolds that are most likely involved in the maintenance of plastid integrity and in plastid division. As these networks resemble the eukaryotic cytoskeleton in form and function, we suggest the term "plastoskeleton" for this newly described subcellular structure.

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Consensus phylogenetic tree of FtsZ amino acid sequences. Branch numbers represent bootstrap values (1,000 replicates). Accession nos. of the sequences are available on request.
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Figure 1: Consensus phylogenetic tree of FtsZ amino acid sequences. Branch numbers represent bootstrap values (1,000 replicates). Accession nos. of the sequences are available on request.

Mentions: 27 deduced FtsZ protein sequences were compared with each other, 14 of them from photosynthetic eukaryotes and 13 from photosynthetically inactive bacteria, mitochondria, and Archaea (Fig. 1). Archaea, Gram+ Eubacteria, Cyanobacteria, nongreen algae, as well as α and γ Proteobacteria form monophyletic clusters. As recently described, the mitochondrial Mallomonas sequence clusters within the α Proteobacteria (Beech et al. 2000).


Visualization of a cytoskeleton-like FtsZ network in chloroplasts.

Kiessling J, Kruse S, Rensing SA, Harter K, Decker EL, Reski R - J. Cell Biol. (2000)

Consensus phylogenetic tree of FtsZ amino acid sequences. Branch numbers represent bootstrap values (1,000 replicates). Accession nos. of the sequences are available on request.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2169431&req=5

Figure 1: Consensus phylogenetic tree of FtsZ amino acid sequences. Branch numbers represent bootstrap values (1,000 replicates). Accession nos. of the sequences are available on request.
Mentions: 27 deduced FtsZ protein sequences were compared with each other, 14 of them from photosynthetic eukaryotes and 13 from photosynthetically inactive bacteria, mitochondria, and Archaea (Fig. 1). Archaea, Gram+ Eubacteria, Cyanobacteria, nongreen algae, as well as α and γ Proteobacteria form monophyletic clusters. As recently described, the mitochondrial Mallomonas sequence clusters within the α Proteobacteria (Beech et al. 2000).

Bottom Line: It has been a long-standing dogma in life sciences that only eukaryotic organisms possess a cytoskeleton.Here, we report two nuclear-encoded plant ftsZ genes which are highly conserved in coding sequence and intron structure.As these networks resemble the eukaryotic cytoskeleton in form and function, we suggest the term "plastoskeleton" for this newly described subcellular structure.

View Article: PubMed Central - PubMed

Affiliation: University of Freiburg, Plant Biotechnology, D-79104 Freiburg, Germany.

ABSTRACT
It has been a long-standing dogma in life sciences that only eukaryotic organisms possess a cytoskeleton. Recently, this belief was questioned by the finding that the bacterial cell division protein FtsZ resembles tubulin in sequence and structure and, thus, may be the progenitor of this major eukaryotic cytoskeletal element. Here, we report two nuclear-encoded plant ftsZ genes which are highly conserved in coding sequence and intron structure. Both their encoded proteins are imported into plastids and there, like in bacteria, they act on the division process in a dose-dependent manner. Whereas in bacteria FtsZ only transiently polymerizes to a ring-like structure, in chloroplasts we identified persistent, highly organized filamentous scaffolds that are most likely involved in the maintenance of plastid integrity and in plastid division. As these networks resemble the eukaryotic cytoskeleton in form and function, we suggest the term "plastoskeleton" for this newly described subcellular structure.

Show MeSH
Related in: MedlinePlus