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Genetic determinants of hyaloid and retinal vasculature in zebrafish.

Alvarez Y, Cederlund ML, Cottell DC, Bill BR, Ekker SC, Torres-Vazquez J, Weinstein BM, Hyde DR, Vihtelic TS, Kennedy BN - BMC Dev. Biol. (2007)

Bottom Line: Similar to the transient hyaloid vasculature in mammalian embryos, vessels are first found attached to the zebrafish lens at 2.5 days post fertilisation.Finally, we identify 9 genes with cell membrane, extracellular matrix and unknown identity that are necessary for zebrafish hyaloid and retinal vasculature development.Zebrafish have a retinal blood supply with a characteristic developmental and adult morphology.

View Article: PubMed Central - HTML - PubMed

Affiliation: UCD School of Biomolecular, and Biomedical Sciences, University College Dublin, Dublin 4, Ireland. yolanda.alvarez@ucd.ie

ABSTRACT

Background: The retinal vasculature is a capillary network of blood vessels that nourishes the inner retina of most mammals. Developmental abnormalities or microvascular complications in the retinal vasculature result in severe human eye diseases that lead to blindness. To exploit the advantages of zebrafish for genetic, developmental and pharmacological studies of retinal vasculature, we characterised the intraocular vasculature in zebrafish.

Results: We show a detailed morphological and developmental analysis of the retinal blood supply in zebrafish. Similar to the transient hyaloid vasculature in mammalian embryos, vessels are first found attached to the zebrafish lens at 2.5 days post fertilisation. These vessels progressively lose contact with the lens and by 30 days post fertilisation adhere to the inner limiting membrane of the juvenile retina. Ultrastructure analysis shows these vessels to exhibit distinctive hallmarks of mammalian retinal vasculature. For example, smooth muscle actin-expressing pericytes are ensheathed by the basal lamina of the blood vessel, and vesicle vacuolar organelles (VVO), subcellular mediators of vessel-retinal nourishment, are present. Finally, we identify 9 genes with cell membrane, extracellular matrix and unknown identity that are necessary for zebrafish hyaloid and retinal vasculature development.

Conclusion: Zebrafish have a retinal blood supply with a characteristic developmental and adult morphology. Abnormalities of these intraocular vessels are easily observed, enabling application of genetic and chemical approaches in zebrafish to identify molecular regulators of hyaloid and retinal vasculature in development and disease.

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Model demarking the stages of retinal vasculature development and genetic determinants in zebrafish. Shown are fluorescent micrographs of partially dissected eyes from Tg(fli1:EGFP) fish showing the hyaloid and retinal vasculature in larvae and adult. Hyaloid vessels first appear attached to the back of the lens at 48 hpf and grow rapidly to reach the front of the lens at 3 dpf. In the adult, vessels are found associated with the inner surface of the retina. Insets are 3D models of this process where vessels have been coloured in green, retinas in pink and lens in white. Genes identified in our analysis to affect retinal vasculature development at different stages are boxed in green and genes unrelated to retinal vessels development are boxed in grey. ECM genes: red; lens/retina genes: blue. CHOR: choroidal vasculature; RPE: retinal pigmented epithelium; NR: neuro-retina; IOC: inner optic circle.
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Figure 6: Model demarking the stages of retinal vasculature development and genetic determinants in zebrafish. Shown are fluorescent micrographs of partially dissected eyes from Tg(fli1:EGFP) fish showing the hyaloid and retinal vasculature in larvae and adult. Hyaloid vessels first appear attached to the back of the lens at 48 hpf and grow rapidly to reach the front of the lens at 3 dpf. In the adult, vessels are found associated with the inner surface of the retina. Insets are 3D models of this process where vessels have been coloured in green, retinas in pink and lens in white. Genes identified in our analysis to affect retinal vasculature development at different stages are boxed in green and genes unrelated to retinal vessels development are boxed in grey. ECM genes: red; lens/retina genes: blue. CHOR: choroidal vasculature; RPE: retinal pigmented epithelium; NR: neuro-retina; IOC: inner optic circle.

Mentions: We describe the cellular and ultrastructural morphology of the hyaloid and retinal vasculature in zebrafish from early development through senescence and identify genes encoding extracellular matrix and cell surface proteins that are required for their development (Fig 6).


Genetic determinants of hyaloid and retinal vasculature in zebrafish.

Alvarez Y, Cederlund ML, Cottell DC, Bill BR, Ekker SC, Torres-Vazquez J, Weinstein BM, Hyde DR, Vihtelic TS, Kennedy BN - BMC Dev. Biol. (2007)

Model demarking the stages of retinal vasculature development and genetic determinants in zebrafish. Shown are fluorescent micrographs of partially dissected eyes from Tg(fli1:EGFP) fish showing the hyaloid and retinal vasculature in larvae and adult. Hyaloid vessels first appear attached to the back of the lens at 48 hpf and grow rapidly to reach the front of the lens at 3 dpf. In the adult, vessels are found associated with the inner surface of the retina. Insets are 3D models of this process where vessels have been coloured in green, retinas in pink and lens in white. Genes identified in our analysis to affect retinal vasculature development at different stages are boxed in green and genes unrelated to retinal vessels development are boxed in grey. ECM genes: red; lens/retina genes: blue. CHOR: choroidal vasculature; RPE: retinal pigmented epithelium; NR: neuro-retina; IOC: inner optic circle.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2169232&req=5

Figure 6: Model demarking the stages of retinal vasculature development and genetic determinants in zebrafish. Shown are fluorescent micrographs of partially dissected eyes from Tg(fli1:EGFP) fish showing the hyaloid and retinal vasculature in larvae and adult. Hyaloid vessels first appear attached to the back of the lens at 48 hpf and grow rapidly to reach the front of the lens at 3 dpf. In the adult, vessels are found associated with the inner surface of the retina. Insets are 3D models of this process where vessels have been coloured in green, retinas in pink and lens in white. Genes identified in our analysis to affect retinal vasculature development at different stages are boxed in green and genes unrelated to retinal vessels development are boxed in grey. ECM genes: red; lens/retina genes: blue. CHOR: choroidal vasculature; RPE: retinal pigmented epithelium; NR: neuro-retina; IOC: inner optic circle.
Mentions: We describe the cellular and ultrastructural morphology of the hyaloid and retinal vasculature in zebrafish from early development through senescence and identify genes encoding extracellular matrix and cell surface proteins that are required for their development (Fig 6).

Bottom Line: Similar to the transient hyaloid vasculature in mammalian embryos, vessels are first found attached to the zebrafish lens at 2.5 days post fertilisation.Finally, we identify 9 genes with cell membrane, extracellular matrix and unknown identity that are necessary for zebrafish hyaloid and retinal vasculature development.Zebrafish have a retinal blood supply with a characteristic developmental and adult morphology.

View Article: PubMed Central - HTML - PubMed

Affiliation: UCD School of Biomolecular, and Biomedical Sciences, University College Dublin, Dublin 4, Ireland. yolanda.alvarez@ucd.ie

ABSTRACT

Background: The retinal vasculature is a capillary network of blood vessels that nourishes the inner retina of most mammals. Developmental abnormalities or microvascular complications in the retinal vasculature result in severe human eye diseases that lead to blindness. To exploit the advantages of zebrafish for genetic, developmental and pharmacological studies of retinal vasculature, we characterised the intraocular vasculature in zebrafish.

Results: We show a detailed morphological and developmental analysis of the retinal blood supply in zebrafish. Similar to the transient hyaloid vasculature in mammalian embryos, vessels are first found attached to the zebrafish lens at 2.5 days post fertilisation. These vessels progressively lose contact with the lens and by 30 days post fertilisation adhere to the inner limiting membrane of the juvenile retina. Ultrastructure analysis shows these vessels to exhibit distinctive hallmarks of mammalian retinal vasculature. For example, smooth muscle actin-expressing pericytes are ensheathed by the basal lamina of the blood vessel, and vesicle vacuolar organelles (VVO), subcellular mediators of vessel-retinal nourishment, are present. Finally, we identify 9 genes with cell membrane, extracellular matrix and unknown identity that are necessary for zebrafish hyaloid and retinal vasculature development.

Conclusion: Zebrafish have a retinal blood supply with a characteristic developmental and adult morphology. Abnormalities of these intraocular vessels are easily observed, enabling application of genetic and chemical approaches in zebrafish to identify molecular regulators of hyaloid and retinal vasculature in development and disease.

Show MeSH
Related in: MedlinePlus