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Impact of salinomycin on the intestinal microflora of broiler chickens.

Johansen CH, Bjerrum L, Pedersen K - Acta Vet. Scand. (2007)

Bottom Line: We found no effect of salinomycin on C. jejuni but salinomycin significantly affected the composition of the microflora.In addition, salinomycin significantly reduced the prevalence of Clostridium perfringens and we observed a significant increase (62%) in the mean body weight of salinomycin treated chickens compared to un-treated controls.Termination of the use of ionophore coccidiostats will not affect food safety related to campylobacter, but will increase the risk of necrotic enteritis in the broilers.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Veterinary Institute, Technical University of Denmark, Hangøvej 2, DK-8200 Aarhus N, Denmark. cjo@vet.dtu.dk

ABSTRACT

Background: The ionophoric coccidiostat salinomycin is widely used in chicken feed. In the near future the use of ionophore coccidiostats may be banned as has been the case for other antimicrobial growth promoters. This study was conducted to examine the effect of salinomycin on Campylobacter jejuni infection and on the composition of the caecal microflora in broiler chickens.

Methods: An experimental infection study was carried out in isolators and the intestinal microflora was analyzed using quantitative cultivation, denaturant gradient gel electrophoresis (DGGE), cloning and sequencing.

Results: We found no effect of salinomycin on C. jejuni but salinomycin significantly affected the composition of the microflora. In addition, salinomycin significantly reduced the prevalence of Clostridium perfringens and we observed a significant increase (62%) in the mean body weight of salinomycin treated chickens compared to un-treated controls.

Conclusion: Termination of the use of ionophore coccidiostats will not affect food safety related to campylobacter, but will increase the risk of necrotic enteritis in the broilers.

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Related in: MedlinePlus

DGGE profile and dendrogram based on caecal samples from 30-day-old chickens. M refers to the molecular marker, containing DNA fragments from 5 pure cultures, as mentioned above. The treatment of the chickens is indicated by, I (infected with Campylobacter jejuni) or S (infected with C. jejuni + salinomycin treated).
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Figure 2: DGGE profile and dendrogram based on caecal samples from 30-day-old chickens. M refers to the molecular marker, containing DNA fragments from 5 pure cultures, as mentioned above. The treatment of the chickens is indicated by, I (infected with Campylobacter jejuni) or S (infected with C. jejuni + salinomycin treated).

Mentions: The DGGE band patterns of samples from day 23 and 30 (Figure 2), clustered into two distinct groups comprising the salinomycin + C. jejuni treated chickens (groups 1 and 2) and the C. jejuni infected chickens (groups 3 and 4). The similarity index between the two groups was approximately 58%. The within-group-similarity index of the salinomycin treated chickens was approximately 72% and the within-group similarity value of the non-treated, C. jejuni infected chickens was approximately 62%. At day 23, a 64% within-group-similarity of the salinomycin treated chickens and a 70% within-group-similarity of the non-treated, C. jejuni infected chickens was found. However one of the C. jejuni infected, non-treated samples represented the base of the dendrogram and showed only 50% similarity to the remaining samples.


Impact of salinomycin on the intestinal microflora of broiler chickens.

Johansen CH, Bjerrum L, Pedersen K - Acta Vet. Scand. (2007)

DGGE profile and dendrogram based on caecal samples from 30-day-old chickens. M refers to the molecular marker, containing DNA fragments from 5 pure cultures, as mentioned above. The treatment of the chickens is indicated by, I (infected with Campylobacter jejuni) or S (infected with C. jejuni + salinomycin treated).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2164938&req=5

Figure 2: DGGE profile and dendrogram based on caecal samples from 30-day-old chickens. M refers to the molecular marker, containing DNA fragments from 5 pure cultures, as mentioned above. The treatment of the chickens is indicated by, I (infected with Campylobacter jejuni) or S (infected with C. jejuni + salinomycin treated).
Mentions: The DGGE band patterns of samples from day 23 and 30 (Figure 2), clustered into two distinct groups comprising the salinomycin + C. jejuni treated chickens (groups 1 and 2) and the C. jejuni infected chickens (groups 3 and 4). The similarity index between the two groups was approximately 58%. The within-group-similarity index of the salinomycin treated chickens was approximately 72% and the within-group similarity value of the non-treated, C. jejuni infected chickens was approximately 62%. At day 23, a 64% within-group-similarity of the salinomycin treated chickens and a 70% within-group-similarity of the non-treated, C. jejuni infected chickens was found. However one of the C. jejuni infected, non-treated samples represented the base of the dendrogram and showed only 50% similarity to the remaining samples.

Bottom Line: We found no effect of salinomycin on C. jejuni but salinomycin significantly affected the composition of the microflora.In addition, salinomycin significantly reduced the prevalence of Clostridium perfringens and we observed a significant increase (62%) in the mean body weight of salinomycin treated chickens compared to un-treated controls.Termination of the use of ionophore coccidiostats will not affect food safety related to campylobacter, but will increase the risk of necrotic enteritis in the broilers.

View Article: PubMed Central - HTML - PubMed

Affiliation: National Veterinary Institute, Technical University of Denmark, Hangøvej 2, DK-8200 Aarhus N, Denmark. cjo@vet.dtu.dk

ABSTRACT

Background: The ionophoric coccidiostat salinomycin is widely used in chicken feed. In the near future the use of ionophore coccidiostats may be banned as has been the case for other antimicrobial growth promoters. This study was conducted to examine the effect of salinomycin on Campylobacter jejuni infection and on the composition of the caecal microflora in broiler chickens.

Methods: An experimental infection study was carried out in isolators and the intestinal microflora was analyzed using quantitative cultivation, denaturant gradient gel electrophoresis (DGGE), cloning and sequencing.

Results: We found no effect of salinomycin on C. jejuni but salinomycin significantly affected the composition of the microflora. In addition, salinomycin significantly reduced the prevalence of Clostridium perfringens and we observed a significant increase (62%) in the mean body weight of salinomycin treated chickens compared to un-treated controls.

Conclusion: Termination of the use of ionophore coccidiostats will not affect food safety related to campylobacter, but will increase the risk of necrotic enteritis in the broilers.

Show MeSH
Related in: MedlinePlus