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Elevated adipogenesis of marrow mesenchymal stem cells during early steroid-associated osteonecrosis development.

Sheng HH, Zhang GG, Cheung WH, Chan CW, Wang YX, Lee KM, Wang HF, Leung KS, Qin LL - J Orthop Surg Res (2007)

Bottom Line: However, it remains unclear whether there is a close association between elevated adipogenesis and steroid-associated ON development.Local intra-osseous perfusion index was found lower in either ON+ or MON group when compared to either ON- or SON group, whereas the marrow fat cells number and area were much larger in either ON+ or MON group as compared with ON- and SON group.The adipogenic differentiation ability of MSCs and PPARgamma2 expression in either ON+ or MON group were elevated significantly as compared with either ON- or SON group.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Orthopaedics & Traumatology, The Chinese University of Hong Kong, Hong Kong, China.

ABSTRACT

Background: Increased bone marrow lipid deposition in steroid-associated osteonecrosis (ON) implies that abnormalities in fat metabolism play an important role in ON development. The increase in lipid deposition might be explained by elevated adipogenesis of marrow mesenchymal stem cells (MSCs). However, it remains unclear whether there is a close association between elevated adipogenesis and steroid-associated ON development.

Objective: The present study was designed to test the hypothesis that there might be a close association between elevated adipogenesis and steroid-associated ON development.

Methods: ON rabbit model was induced based on our established protocol. Dynamic-MRI was employed for local intra-osseous perfusion evaluation in bilateral femora. Two weeks after induction, bone marrow was harvested for evaluating the ability of adipogenic differentiation of marrow MSCs at both cellular and mRNA level involving adipogenesis-related gene peroxisome proliferator-activated receptor gamma2 (PPARgamma2). The bilateral femora were dissected for examining marrow lipid deposition by quantifying fat cell number, fat cell size, lipid deposition area and ON lesions. For investigating association among adipogenesis, lipid deposition and perfusion function with regard to ON occurrence, the rabbits were divided into ON+ (with at least one ON lesion) group and ON- (without ON lesion) group. For investigating association among adipogenesis, lipid deposition and perfusion function with regard to ON extension, the ON+ rabbits were further divided into sub-single-lesion group (SON group: with one ON lesion) and sub-multiple-lesion group (MON group: with more than one ON lesion).

Results: Local intra-osseous perfusion index was found lower in either ON+ or MON group when compared to either ON- or SON group, whereas the marrow fat cells number and area were much larger in either ON+ or MON group as compared with ON- and SON group. The adipogenic differentiation ability of MSCs and PPARgamma2 expression in either ON+ or MON group were elevated significantly as compared with either ON- or SON group.

Conclusion: These findings support our hypothesis that there is a close association between elevated adipogenesis and steroid-associated osteonecrosis development.

No MeSH data available.


Related in: MedlinePlus

Adipogenesis of MSCs from different groups. Representative pictures showing much more adipocyte-like cells formation in ON+ group (B) than ON- group (A)(Oil Red O staining 100×); (C) Quantification result showed much more lipid droplets in ON+ and MON group as compared with ON- and SON group (p < 0.05).
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Figure 4: Adipogenesis of MSCs from different groups. Representative pictures showing much more adipocyte-like cells formation in ON+ group (B) than ON- group (A)(Oil Red O staining 100×); (C) Quantification result showed much more lipid droplets in ON+ and MON group as compared with ON- and SON group (p < 0.05).

Mentions: The cells accumulated triglycerides vesicles, that was small initially and increased in size with time. The number of adipocytes in ON+ group was 270% more as compared with ON- group, 120% more in MON group as compared with SON group(p < 0.05). The optical density results showed 210% more triglycerides formation in ON+ group as compared with ON- group, and 80% more in MON group as compared with SON group (p < 0.05) (Figure 4).


Elevated adipogenesis of marrow mesenchymal stem cells during early steroid-associated osteonecrosis development.

Sheng HH, Zhang GG, Cheung WH, Chan CW, Wang YX, Lee KM, Wang HF, Leung KS, Qin LL - J Orthop Surg Res (2007)

Adipogenesis of MSCs from different groups. Representative pictures showing much more adipocyte-like cells formation in ON+ group (B) than ON- group (A)(Oil Red O staining 100×); (C) Quantification result showed much more lipid droplets in ON+ and MON group as compared with ON- and SON group (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2146995&req=5

Figure 4: Adipogenesis of MSCs from different groups. Representative pictures showing much more adipocyte-like cells formation in ON+ group (B) than ON- group (A)(Oil Red O staining 100×); (C) Quantification result showed much more lipid droplets in ON+ and MON group as compared with ON- and SON group (p < 0.05).
Mentions: The cells accumulated triglycerides vesicles, that was small initially and increased in size with time. The number of adipocytes in ON+ group was 270% more as compared with ON- group, 120% more in MON group as compared with SON group(p < 0.05). The optical density results showed 210% more triglycerides formation in ON+ group as compared with ON- group, and 80% more in MON group as compared with SON group (p < 0.05) (Figure 4).

Bottom Line: However, it remains unclear whether there is a close association between elevated adipogenesis and steroid-associated ON development.Local intra-osseous perfusion index was found lower in either ON+ or MON group when compared to either ON- or SON group, whereas the marrow fat cells number and area were much larger in either ON+ or MON group as compared with ON- and SON group.The adipogenic differentiation ability of MSCs and PPARgamma2 expression in either ON+ or MON group were elevated significantly as compared with either ON- or SON group.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Orthopaedics & Traumatology, The Chinese University of Hong Kong, Hong Kong, China.

ABSTRACT

Background: Increased bone marrow lipid deposition in steroid-associated osteonecrosis (ON) implies that abnormalities in fat metabolism play an important role in ON development. The increase in lipid deposition might be explained by elevated adipogenesis of marrow mesenchymal stem cells (MSCs). However, it remains unclear whether there is a close association between elevated adipogenesis and steroid-associated ON development.

Objective: The present study was designed to test the hypothesis that there might be a close association between elevated adipogenesis and steroid-associated ON development.

Methods: ON rabbit model was induced based on our established protocol. Dynamic-MRI was employed for local intra-osseous perfusion evaluation in bilateral femora. Two weeks after induction, bone marrow was harvested for evaluating the ability of adipogenic differentiation of marrow MSCs at both cellular and mRNA level involving adipogenesis-related gene peroxisome proliferator-activated receptor gamma2 (PPARgamma2). The bilateral femora were dissected for examining marrow lipid deposition by quantifying fat cell number, fat cell size, lipid deposition area and ON lesions. For investigating association among adipogenesis, lipid deposition and perfusion function with regard to ON occurrence, the rabbits were divided into ON+ (with at least one ON lesion) group and ON- (without ON lesion) group. For investigating association among adipogenesis, lipid deposition and perfusion function with regard to ON extension, the ON+ rabbits were further divided into sub-single-lesion group (SON group: with one ON lesion) and sub-multiple-lesion group (MON group: with more than one ON lesion).

Results: Local intra-osseous perfusion index was found lower in either ON+ or MON group when compared to either ON- or SON group, whereas the marrow fat cells number and area were much larger in either ON+ or MON group as compared with ON- and SON group. The adipogenic differentiation ability of MSCs and PPARgamma2 expression in either ON+ or MON group were elevated significantly as compared with either ON- or SON group.

Conclusion: These findings support our hypothesis that there is a close association between elevated adipogenesis and steroid-associated osteonecrosis development.

No MeSH data available.


Related in: MedlinePlus