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Substrate recognition by osteoclast precursors induces C-src/microtubule association.

Abu-Amer Y, Ross FP, Schlesinger P, Tondravi MM, Teitelbaum SL - J. Cell Biol. (1997)

Bottom Line: In vitro kinase assay demonstrates tubulin-associated c-src is enzymatically active, phosphorylating itself as well as exogenous substrate.The increase in microtubule-associated kinase activity attending adhesion mirrors tubulin-bound c-src and does not reflect enhanced specific activity.Association of the two proteins does not depend upon protein tyrosine phosphorylation and is substrate specific, as it is induced by vitronectin and fibronectin but not type 1 collagen.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

ABSTRACT
The osteoclast is distinguished from other macrophage polykaryons by its polarization, a feature induced by substrate recognition. The most striking component of the polarized osteoclast is its ruffled membrane, probably reflecting insertion of intracellular vesicles into the bone apposed plasmalemma. The failure of osteoclasts in c-src-/- osteopetrotic mice to form ruffled membranes indicates pp60(c-src) (c-src) is essential to osteoclast polarization. Interestingly, c-src itself is a vesicular protein that targets the ruffled membrane. This being the case, we hypothesized that matrix recognition by osteoclasts, and their precursors, induces c-src to associate with microtubules that traffic proteins to the cell surface. We find abundant c-src associates with tubulin immunoprecipitated from avian marrow macrophages (osteoclast precursors) maintained in the adherent, but not nonadherent, state. Since the two proteins colocalize only within adherent avian osteoclast-like cells examined by double antibody immunoconfocal microscopy, c-src/tubulin association reflects an authentic intracellular event. C-src/tubulin association is evident within 90 min of cell-substrate recognition, and the event does not reflect increased expression of either protein. In vitro kinase assay demonstrates tubulin-associated c-src is enzymatically active, phosphorylating itself as well as exogenous substrate. The increase in microtubule-associated kinase activity attending adhesion mirrors tubulin-bound c-src and does not reflect enhanced specific activity. The fact that microtubule-dissociating drugs, as well as cold, prevent adherence-induced c-src/tubulin association indicates the protooncogene complexes primarily, if not exclusively, with polymerized tubulin. Association of the two proteins does not depend upon protein tyrosine phosphorylation and is substrate specific, as it is induced by vitronectin and fibronectin but not type 1 collagen. Finally, consistent with cotransport of c-src and the osteoclast vacuolar proton pump to the polarized plasmalemma, the H+-ATPase decorates microtubules in a manner similar to the protooncogene, specifically coimmunoprecipitates with c-src from the osteoclast light Golgi membrane fraction, and is present, with c-src, in preparations enriched with acidifying vesicles reconstituted from the osteoclast ruffled membrane.

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Tubulin and c-src proteins coimmunoprecipitate in  avian marrow macrophages. Adherent macrophages were either  lysed directly in sample buffer (lane 5) or in lysis buffer and immunoprecipitated with either anti–c-src (lane 2) or antitubulin  antibody (lane 3). Lysate and immunoprecipitates were subjected  to SDS-PAGE, and the gel was stained with Coomassie blue.  Lane 1 contains sample buffer, and lane 4 contains a positive control of enriched c-src protein extract.
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Figure 1: Tubulin and c-src proteins coimmunoprecipitate in avian marrow macrophages. Adherent macrophages were either lysed directly in sample buffer (lane 5) or in lysis buffer and immunoprecipitated with either anti–c-src (lane 2) or antitubulin antibody (lane 3). Lysate and immunoprecipitates were subjected to SDS-PAGE, and the gel was stained with Coomassie blue. Lane 1 contains sample buffer, and lane 4 contains a positive control of enriched c-src protein extract.

Mentions: The osteoclast ruffled membrane is rich in c-src (49), and as such, a reasonable hypothesis holds that microtubules mediate transport of the protooncogene to the cell surface. Thus, we asked if c-src and tubulin associate in avian osteoclast progenitors. As seen in Fig. 1, lysate derived from marrow macrophages contains proteins comigrating with c-src and tubulin (lane 5). Furthermore, while numerous other proteins are also evident, bands of tubulin and c-src are present in lysate immunoprecipitated with either anti– c-src (lane 2) or antitubulin (lane 3) antibodies. In this and all subsequent figures, immunoblots were performed in the linear range of the ECL detection system (Fig. 2).


Substrate recognition by osteoclast precursors induces C-src/microtubule association.

Abu-Amer Y, Ross FP, Schlesinger P, Tondravi MM, Teitelbaum SL - J. Cell Biol. (1997)

Tubulin and c-src proteins coimmunoprecipitate in  avian marrow macrophages. Adherent macrophages were either  lysed directly in sample buffer (lane 5) or in lysis buffer and immunoprecipitated with either anti–c-src (lane 2) or antitubulin  antibody (lane 3). Lysate and immunoprecipitates were subjected  to SDS-PAGE, and the gel was stained with Coomassie blue.  Lane 1 contains sample buffer, and lane 4 contains a positive control of enriched c-src protein extract.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2139850&req=5

Figure 1: Tubulin and c-src proteins coimmunoprecipitate in avian marrow macrophages. Adherent macrophages were either lysed directly in sample buffer (lane 5) or in lysis buffer and immunoprecipitated with either anti–c-src (lane 2) or antitubulin antibody (lane 3). Lysate and immunoprecipitates were subjected to SDS-PAGE, and the gel was stained with Coomassie blue. Lane 1 contains sample buffer, and lane 4 contains a positive control of enriched c-src protein extract.
Mentions: The osteoclast ruffled membrane is rich in c-src (49), and as such, a reasonable hypothesis holds that microtubules mediate transport of the protooncogene to the cell surface. Thus, we asked if c-src and tubulin associate in avian osteoclast progenitors. As seen in Fig. 1, lysate derived from marrow macrophages contains proteins comigrating with c-src and tubulin (lane 5). Furthermore, while numerous other proteins are also evident, bands of tubulin and c-src are present in lysate immunoprecipitated with either anti– c-src (lane 2) or antitubulin (lane 3) antibodies. In this and all subsequent figures, immunoblots were performed in the linear range of the ECL detection system (Fig. 2).

Bottom Line: In vitro kinase assay demonstrates tubulin-associated c-src is enzymatically active, phosphorylating itself as well as exogenous substrate.The increase in microtubule-associated kinase activity attending adhesion mirrors tubulin-bound c-src and does not reflect enhanced specific activity.Association of the two proteins does not depend upon protein tyrosine phosphorylation and is substrate specific, as it is induced by vitronectin and fibronectin but not type 1 collagen.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

ABSTRACT
The osteoclast is distinguished from other macrophage polykaryons by its polarization, a feature induced by substrate recognition. The most striking component of the polarized osteoclast is its ruffled membrane, probably reflecting insertion of intracellular vesicles into the bone apposed plasmalemma. The failure of osteoclasts in c-src-/- osteopetrotic mice to form ruffled membranes indicates pp60(c-src) (c-src) is essential to osteoclast polarization. Interestingly, c-src itself is a vesicular protein that targets the ruffled membrane. This being the case, we hypothesized that matrix recognition by osteoclasts, and their precursors, induces c-src to associate with microtubules that traffic proteins to the cell surface. We find abundant c-src associates with tubulin immunoprecipitated from avian marrow macrophages (osteoclast precursors) maintained in the adherent, but not nonadherent, state. Since the two proteins colocalize only within adherent avian osteoclast-like cells examined by double antibody immunoconfocal microscopy, c-src/tubulin association reflects an authentic intracellular event. C-src/tubulin association is evident within 90 min of cell-substrate recognition, and the event does not reflect increased expression of either protein. In vitro kinase assay demonstrates tubulin-associated c-src is enzymatically active, phosphorylating itself as well as exogenous substrate. The increase in microtubule-associated kinase activity attending adhesion mirrors tubulin-bound c-src and does not reflect enhanced specific activity. The fact that microtubule-dissociating drugs, as well as cold, prevent adherence-induced c-src/tubulin association indicates the protooncogene complexes primarily, if not exclusively, with polymerized tubulin. Association of the two proteins does not depend upon protein tyrosine phosphorylation and is substrate specific, as it is induced by vitronectin and fibronectin but not type 1 collagen. Finally, consistent with cotransport of c-src and the osteoclast vacuolar proton pump to the polarized plasmalemma, the H+-ATPase decorates microtubules in a manner similar to the protooncogene, specifically coimmunoprecipitates with c-src from the osteoclast light Golgi membrane fraction, and is present, with c-src, in preparations enriched with acidifying vesicles reconstituted from the osteoclast ruffled membrane.

Show MeSH
Related in: MedlinePlus