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Differentiation and death of premyelinating oligodendrocytes in developing rodent brain.

Trapp BD, Nishiyama A, Cheng D, Macklin W - J. Cell Biol. (1997)

Bottom Line: These premyelinating oligodendrocytes have one of two fates: they myelinate axons or degenerate.Between 7 and 21 d after birth, approximately 20% of premyelinating oligodendrocytes identified in the cerebral cortex were degenerating.Oligodendrocytes that ensheathed axons expressed and selectively targeted proteolipid protein to compact myelin and did not degenerate.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosciences, Research Institute, The Cleveland Clinic Foundation, Ohio 44195, USA.

ABSTRACT
Previous studies have indicated that newly formed oligodendrocytes are dynamic cells whose production, survival, and differentiation depend upon axonal influences. This study has characterized the appearance and fate of newly formed oligodendrocytes in developing rat brain. Oligodendrocytes appear in predictable locations and radially extend DM-20-positive processes that cover 80-microm domains in the cortex and 40-microm domains in the corpus callosum. These premyelinating oligodendrocytes have one of two fates: they myelinate axons or degenerate. Between 7 and 21 d after birth, approximately 20% of premyelinating oligodendrocytes identified in the cerebral cortex were degenerating. Oligodendrocytes that ensheathed axons expressed and selectively targeted proteolipid protein to compact myelin and did not degenerate. These observations support the hypothesis that axonal influences affect oligodendrocyte survival, differentiation, and expression of proteolipid protein gene products.

Show MeSH
Schematic summary of oligodendrocyte lineage in vivo. Progenitor cells  produce premyelinating oligodendrocytes. Premyelinating oligodendrocytes occupy  distinct neuropil domains  and depend on axonal signal(s) for survival. Images are  two-dimensional reconstructions of confocal images.
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Figure 8: Schematic summary of oligodendrocyte lineage in vivo. Progenitor cells produce premyelinating oligodendrocytes. Premyelinating oligodendrocytes occupy distinct neuropil domains and depend on axonal signal(s) for survival. Images are two-dimensional reconstructions of confocal images.

Mentions: Based on the distribution of PLP and DM-20, this study provides additional insights into the molecular and cellular events that occur during oligodendrocyte differentiation in vivo. Our studies confirm that a significant percentage of newly formed oligodendrocytes degenerate and provide the first description of their location and phenotype. Oligodendrocytes that degenerate express DM-20 and do not ensheath axons or express detectable levels of PLP, the major structural protein of compact myelin. These observations are consistent with the hypothesis that oligodendrocyte cell death occurs before any significant commitment to myelin formation and that axonal ensheathment is essential for oligodendrocyte survival. Fig. 8 schematically summarizes stages and features of oligodendrocyte differentiation in vivo and is based on differential detection of DM-20 and PLP in confocal images. This model supports and extends those proposed previously (Hardy and Reynolds, 1991; Warrington and Pfeiffer, 1992; Ffrench-Constant, 1992; Barres and Raff, 1994) and is based on direct analysis of cells in vivo.


Differentiation and death of premyelinating oligodendrocytes in developing rodent brain.

Trapp BD, Nishiyama A, Cheng D, Macklin W - J. Cell Biol. (1997)

Schematic summary of oligodendrocyte lineage in vivo. Progenitor cells  produce premyelinating oligodendrocytes. Premyelinating oligodendrocytes occupy  distinct neuropil domains  and depend on axonal signal(s) for survival. Images are  two-dimensional reconstructions of confocal images.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2139778&req=5

Figure 8: Schematic summary of oligodendrocyte lineage in vivo. Progenitor cells produce premyelinating oligodendrocytes. Premyelinating oligodendrocytes occupy distinct neuropil domains and depend on axonal signal(s) for survival. Images are two-dimensional reconstructions of confocal images.
Mentions: Based on the distribution of PLP and DM-20, this study provides additional insights into the molecular and cellular events that occur during oligodendrocyte differentiation in vivo. Our studies confirm that a significant percentage of newly formed oligodendrocytes degenerate and provide the first description of their location and phenotype. Oligodendrocytes that degenerate express DM-20 and do not ensheath axons or express detectable levels of PLP, the major structural protein of compact myelin. These observations are consistent with the hypothesis that oligodendrocyte cell death occurs before any significant commitment to myelin formation and that axonal ensheathment is essential for oligodendrocyte survival. Fig. 8 schematically summarizes stages and features of oligodendrocyte differentiation in vivo and is based on differential detection of DM-20 and PLP in confocal images. This model supports and extends those proposed previously (Hardy and Reynolds, 1991; Warrington and Pfeiffer, 1992; Ffrench-Constant, 1992; Barres and Raff, 1994) and is based on direct analysis of cells in vivo.

Bottom Line: These premyelinating oligodendrocytes have one of two fates: they myelinate axons or degenerate.Between 7 and 21 d after birth, approximately 20% of premyelinating oligodendrocytes identified in the cerebral cortex were degenerating.Oligodendrocytes that ensheathed axons expressed and selectively targeted proteolipid protein to compact myelin and did not degenerate.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosciences, Research Institute, The Cleveland Clinic Foundation, Ohio 44195, USA.

ABSTRACT
Previous studies have indicated that newly formed oligodendrocytes are dynamic cells whose production, survival, and differentiation depend upon axonal influences. This study has characterized the appearance and fate of newly formed oligodendrocytes in developing rat brain. Oligodendrocytes appear in predictable locations and radially extend DM-20-positive processes that cover 80-microm domains in the cortex and 40-microm domains in the corpus callosum. These premyelinating oligodendrocytes have one of two fates: they myelinate axons or degenerate. Between 7 and 21 d after birth, approximately 20% of premyelinating oligodendrocytes identified in the cerebral cortex were degenerating. Oligodendrocytes that ensheathed axons expressed and selectively targeted proteolipid protein to compact myelin and did not degenerate. These observations support the hypothesis that axonal influences affect oligodendrocyte survival, differentiation, and expression of proteolipid protein gene products.

Show MeSH