Limits...
Differentiation and death of premyelinating oligodendrocytes in developing rodent brain.

Trapp BD, Nishiyama A, Cheng D, Macklin W - J. Cell Biol. (1997)

Bottom Line: These premyelinating oligodendrocytes have one of two fates: they myelinate axons or degenerate.Between 7 and 21 d after birth, approximately 20% of premyelinating oligodendrocytes identified in the cerebral cortex were degenerating.Oligodendrocytes that ensheathed axons expressed and selectively targeted proteolipid protein to compact myelin and did not degenerate.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosciences, Research Institute, The Cleveland Clinic Foundation, Ohio 44195, USA.

ABSTRACT
Previous studies have indicated that newly formed oligodendrocytes are dynamic cells whose production, survival, and differentiation depend upon axonal influences. This study has characterized the appearance and fate of newly formed oligodendrocytes in developing rat brain. Oligodendrocytes appear in predictable locations and radially extend DM-20-positive processes that cover 80-microm domains in the cortex and 40-microm domains in the corpus callosum. These premyelinating oligodendrocytes have one of two fates: they myelinate axons or degenerate. Between 7 and 21 d after birth, approximately 20% of premyelinating oligodendrocytes identified in the cerebral cortex were degenerating. Oligodendrocytes that ensheathed axons expressed and selectively targeted proteolipid protein to compact myelin and did not degenerate. These observations support the hypothesis that axonal influences affect oligodendrocyte survival, differentiation, and expression of proteolipid protein gene products.

Show MeSH
Premyelinating oligodendrocytes are generated from NG2-positive progenitor cells. Confocal images of sections (30-μmthick) from 7-d-old rat cerebral cortices that were immunostained with NG2 (green) and DM-20/PLP (red) antibodies. A network of  NG2-positive cells covers the cortical neuropil (A, green). DM-20/PLP–positive premyelinating cells are less abundant (A, red). A small  but consistent population of cells are weakly stained by both NG2 and DM-20/PLP antibodies (B and C, arrowheads). NG2 immunoreactivity is punctate on the plasma membrane of these cells, whereas DM-20/PLP appears punctate in perinuclear cytoplasm on proximal  cell processes (B and C). Bars, 10 μm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2139778&req=5

Figure 7: Premyelinating oligodendrocytes are generated from NG2-positive progenitor cells. Confocal images of sections (30-μmthick) from 7-d-old rat cerebral cortices that were immunostained with NG2 (green) and DM-20/PLP (red) antibodies. A network of NG2-positive cells covers the cortical neuropil (A, green). DM-20/PLP–positive premyelinating cells are less abundant (A, red). A small but consistent population of cells are weakly stained by both NG2 and DM-20/PLP antibodies (B and C, arrowheads). NG2 immunoreactivity is punctate on the plasma membrane of these cells, whereas DM-20/PLP appears punctate in perinuclear cytoplasm on proximal cell processes (B and C). Bars, 10 μm.

Mentions: To investigate if DM-20/PLP–positive cells originate from NG2-positive cells or from another cell that is committed to the oligodendrocyte lineage, sections were double-labeled with NG2 and DM-20/PLP antibodies and examined by confocal microscopy. In sections from 3-d-old brain, NG2positive cells were present throughout the cerebral cortex, but few DM-20/PLP-positive cells were detected (data not shown). Sections from 7-d-old brain contained significant numbers of DM-20/PLP–positive premyelinating oligodendrocytes (Fig. 7). While most cells were stained with either NG2 or DM-20/PLP antibodies (Fig. 7 A), a small but consistent population of cells was stained by both antibodies (Fig. 7, B and C). Cells positive for both NG2 and DM-20/PLP were identified by weak DM-20/PLP immunoreactivity in perinuclear cytoplasm and on proximal processes. When both channels were imaged, the intensity of NG2 and DM-20/PLP immunoreactivity in double-labeled cells was much weaker than that detected in surrounding cells, which were either NG2 or DM-20/PLP positive. These observations provide additional support that NG2positive progenitor cells produce oligodendrocytes in vivo (Levine et al., 1993; Nishiyama et al., 1996a).


Differentiation and death of premyelinating oligodendrocytes in developing rodent brain.

Trapp BD, Nishiyama A, Cheng D, Macklin W - J. Cell Biol. (1997)

Premyelinating oligodendrocytes are generated from NG2-positive progenitor cells. Confocal images of sections (30-μmthick) from 7-d-old rat cerebral cortices that were immunostained with NG2 (green) and DM-20/PLP (red) antibodies. A network of  NG2-positive cells covers the cortical neuropil (A, green). DM-20/PLP–positive premyelinating cells are less abundant (A, red). A small  but consistent population of cells are weakly stained by both NG2 and DM-20/PLP antibodies (B and C, arrowheads). NG2 immunoreactivity is punctate on the plasma membrane of these cells, whereas DM-20/PLP appears punctate in perinuclear cytoplasm on proximal  cell processes (B and C). Bars, 10 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2139778&req=5

Figure 7: Premyelinating oligodendrocytes are generated from NG2-positive progenitor cells. Confocal images of sections (30-μmthick) from 7-d-old rat cerebral cortices that were immunostained with NG2 (green) and DM-20/PLP (red) antibodies. A network of NG2-positive cells covers the cortical neuropil (A, green). DM-20/PLP–positive premyelinating cells are less abundant (A, red). A small but consistent population of cells are weakly stained by both NG2 and DM-20/PLP antibodies (B and C, arrowheads). NG2 immunoreactivity is punctate on the plasma membrane of these cells, whereas DM-20/PLP appears punctate in perinuclear cytoplasm on proximal cell processes (B and C). Bars, 10 μm.
Mentions: To investigate if DM-20/PLP–positive cells originate from NG2-positive cells or from another cell that is committed to the oligodendrocyte lineage, sections were double-labeled with NG2 and DM-20/PLP antibodies and examined by confocal microscopy. In sections from 3-d-old brain, NG2positive cells were present throughout the cerebral cortex, but few DM-20/PLP-positive cells were detected (data not shown). Sections from 7-d-old brain contained significant numbers of DM-20/PLP–positive premyelinating oligodendrocytes (Fig. 7). While most cells were stained with either NG2 or DM-20/PLP antibodies (Fig. 7 A), a small but consistent population of cells was stained by both antibodies (Fig. 7, B and C). Cells positive for both NG2 and DM-20/PLP were identified by weak DM-20/PLP immunoreactivity in perinuclear cytoplasm and on proximal processes. When both channels were imaged, the intensity of NG2 and DM-20/PLP immunoreactivity in double-labeled cells was much weaker than that detected in surrounding cells, which were either NG2 or DM-20/PLP positive. These observations provide additional support that NG2positive progenitor cells produce oligodendrocytes in vivo (Levine et al., 1993; Nishiyama et al., 1996a).

Bottom Line: These premyelinating oligodendrocytes have one of two fates: they myelinate axons or degenerate.Between 7 and 21 d after birth, approximately 20% of premyelinating oligodendrocytes identified in the cerebral cortex were degenerating.Oligodendrocytes that ensheathed axons expressed and selectively targeted proteolipid protein to compact myelin and did not degenerate.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosciences, Research Institute, The Cleveland Clinic Foundation, Ohio 44195, USA.

ABSTRACT
Previous studies have indicated that newly formed oligodendrocytes are dynamic cells whose production, survival, and differentiation depend upon axonal influences. This study has characterized the appearance and fate of newly formed oligodendrocytes in developing rat brain. Oligodendrocytes appear in predictable locations and radially extend DM-20-positive processes that cover 80-microm domains in the cortex and 40-microm domains in the corpus callosum. These premyelinating oligodendrocytes have one of two fates: they myelinate axons or degenerate. Between 7 and 21 d after birth, approximately 20% of premyelinating oligodendrocytes identified in the cerebral cortex were degenerating. Oligodendrocytes that ensheathed axons expressed and selectively targeted proteolipid protein to compact myelin and did not degenerate. These observations support the hypothesis that axonal influences affect oligodendrocyte survival, differentiation, and expression of proteolipid protein gene products.

Show MeSH