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Identification of a bipotential precursor cell in hepatic cell lines derived from transgenic mice expressing cyto-Met in the liver.

Spagnoli FM, Amicone L, Tripodi M, Weiss MC - J. Cell Biol. (1998)

Bottom Line: Palmate cells show none of these properties.Derivation of epithelial from palmate cells is confirmed by characterization of the progeny of individually fished cells.The clonal isolation of the palmate cell, an immortalized nontransformed bipotential cell that does not yet express the liver-enriched transcription factors and is a precursor of the epithelial-hepatocyte in MMH lines, provides a new tool for the study of mechanisms controlling liver development.

View Article: PubMed Central - PubMed

Affiliation: Unité de Génétique de la Différenciation, URA 1773 du Centre National de la Recherche Scientifique, Institut Pasteur, 75724 Paris Cedex 15, France.

ABSTRACT
Met murine hepatocyte (MMH) lines were established from livers of transgenic mice expressing constitutively active human Met. These lines harbor two cell types: epithelial cells resembling the parental populations and flattened cells with multiple projections and a dispersed growth habit that are designated palmate. Epithelial cells express the liver-enriched transcription factors HNF4 and HNF1alpha, and proteins associated with epithelial cell differentiation. Treatments that modulate their differentiation state, including acidic FGF, induce hepatic functions. Palmate cells show none of these properties. However, they can differentiate along the hepatic cell lineage, giving rise to: (a) epithelial cells that express hepatic transcription factors and are competent to express hepatic functions; (b) bile duct-like structures in three-dimensional Matrigel cultures. Derivation of epithelial from palmate cells is confirmed by characterization of the progeny of individually fished cells. Furthermore, karyotype analysis confirms the direction of the phenotypic transition: palmate cells are diploid and the epithelial cells are hypotetraploid. The clonal isolation of the palmate cell, an immortalized nontransformed bipotential cell that does not yet express the liver-enriched transcription factors and is a precursor of the epithelial-hepatocyte in MMH lines, provides a new tool for the study of mechanisms controlling liver development.

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Characterization of  the profile of liver-specific  gene expression in MMH  lines and their clones. Northern blot analysis of total  RNA from the E14, D2, and  D3 parental populations  (pop.), from the epithelial  (ep.) and palmate (pal.)  clones, and from the control  Fao rat hepatoma line (c).  Each lane was loaded with 20  μg of total RNA. Probes  used are indicated on the left.
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Figure 3: Characterization of the profile of liver-specific gene expression in MMH lines and their clones. Northern blot analysis of total RNA from the E14, D2, and D3 parental populations (pop.), from the epithelial (ep.) and palmate (pal.) clones, and from the control Fao rat hepatoma line (c). Each lane was loaded with 20 μg of total RNA. Probes used are indicated on the left.

Mentions: To document differences in gene expression underlying the respective morphological types, RNA was prepared from the three MMH parental lines (E14, D2, and D3) and the seven clones. Blots were prepared and hybridized with probes for LETF, for hepatic functions and for hepatocyte-specific CK 8 and 18 (Fig. 3).


Identification of a bipotential precursor cell in hepatic cell lines derived from transgenic mice expressing cyto-Met in the liver.

Spagnoli FM, Amicone L, Tripodi M, Weiss MC - J. Cell Biol. (1998)

Characterization of  the profile of liver-specific  gene expression in MMH  lines and their clones. Northern blot analysis of total  RNA from the E14, D2, and  D3 parental populations  (pop.), from the epithelial  (ep.) and palmate (pal.)  clones, and from the control  Fao rat hepatoma line (c).  Each lane was loaded with 20  μg of total RNA. Probes  used are indicated on the left.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2132947&req=5

Figure 3: Characterization of the profile of liver-specific gene expression in MMH lines and their clones. Northern blot analysis of total RNA from the E14, D2, and D3 parental populations (pop.), from the epithelial (ep.) and palmate (pal.) clones, and from the control Fao rat hepatoma line (c). Each lane was loaded with 20 μg of total RNA. Probes used are indicated on the left.
Mentions: To document differences in gene expression underlying the respective morphological types, RNA was prepared from the three MMH parental lines (E14, D2, and D3) and the seven clones. Blots were prepared and hybridized with probes for LETF, for hepatic functions and for hepatocyte-specific CK 8 and 18 (Fig. 3).

Bottom Line: Palmate cells show none of these properties.Derivation of epithelial from palmate cells is confirmed by characterization of the progeny of individually fished cells.The clonal isolation of the palmate cell, an immortalized nontransformed bipotential cell that does not yet express the liver-enriched transcription factors and is a precursor of the epithelial-hepatocyte in MMH lines, provides a new tool for the study of mechanisms controlling liver development.

View Article: PubMed Central - PubMed

Affiliation: Unité de Génétique de la Différenciation, URA 1773 du Centre National de la Recherche Scientifique, Institut Pasteur, 75724 Paris Cedex 15, France.

ABSTRACT
Met murine hepatocyte (MMH) lines were established from livers of transgenic mice expressing constitutively active human Met. These lines harbor two cell types: epithelial cells resembling the parental populations and flattened cells with multiple projections and a dispersed growth habit that are designated palmate. Epithelial cells express the liver-enriched transcription factors HNF4 and HNF1alpha, and proteins associated with epithelial cell differentiation. Treatments that modulate their differentiation state, including acidic FGF, induce hepatic functions. Palmate cells show none of these properties. However, they can differentiate along the hepatic cell lineage, giving rise to: (a) epithelial cells that express hepatic transcription factors and are competent to express hepatic functions; (b) bile duct-like structures in three-dimensional Matrigel cultures. Derivation of epithelial from palmate cells is confirmed by characterization of the progeny of individually fished cells. Furthermore, karyotype analysis confirms the direction of the phenotypic transition: palmate cells are diploid and the epithelial cells are hypotetraploid. The clonal isolation of the palmate cell, an immortalized nontransformed bipotential cell that does not yet express the liver-enriched transcription factors and is a precursor of the epithelial-hepatocyte in MMH lines, provides a new tool for the study of mechanisms controlling liver development.

Show MeSH
Related in: MedlinePlus